Unveiling the Potential of Lactic Acid Bacteria from Algerian Dromedary Camel Milk: Diversity, Technological Applications, and Antimicrobial Insights

Luca Rastrelli^1,2*Bilal Latreche^3*Esma Bendjama⁴Lotfi Loucif⁵Ibtissem Sanah⁶Mohammed Messaoudi⁷Chawki Bensouici⁸Fairouz Djeghim³Jean-Marc Rolain⁹Khawla Kerbab¹⁰Maria D'elia²Samira Becila⁶

• ¹University of Salerno, Fisciano, Italy
• ²Universita degli Studi di Salerno Dipartimento di Farmacia, Fisciano, Italy
• ³Universite Freres Mentouri Constantine 1 Institut de la Nutrition de l'Alimentation et des Technologies Agro-Alimentaires, Constantine, Algeria
• ⁴Universite de Batna 1 Hadj Lakhdar, Batna, Algeria
• ⁵Universite Batna 2, Fesdis, Algeria
• ⁶Universite Freres Mentouri Constantine 1, Constantine, Algeria
• ⁷Centre de Recherche Nucleaire de Birine, Djelfa, Algeria
• ⁸Centre de Recherche en Biotechnologie, Constantine, Algeria
• ⁹Aix-Marseille Universite, Marseille, France
• ¹⁰Universite Larbi Ben M'hidi Oum El Bouaghi, Oum el Bouaghi ‎, Algeria

Background: Lactic acid bacteria (LAB) play a central role in the food industry due to their ability to produce beneficial metabolites and enhance the technological and sensory qualities of fermented products. Additionally, they contribute to human health by supporting immune function and maintaining gut microbiota balance through probiotic effects. This study aimed to isolate and characterize LAB from dromedary camel milk (DCM) collected in semi-arid regions of Algeria, evaluating their technological functionalities and antimicrobial activities.Methods: A total of 31 LAB strains were isolated from raw DCM samples. Strains were identified using MALDI-TOF MS and characterized for acidification kinetics, lipolytic, proteolytic, and amylolytic activities, exopolysaccharide (EPS) and acetoin production, and antimicrobial properties against common foodborne pathogens.Results: Four species were identified, with Enterococcus italicus reported for the first time in this environment. Significant inter-strain variability (p < 0.0001) was observed in all tested properties. Three strains (BLC9, BLC12, BLC14) acidified milk rapidly to pH 4.6 within 12 hours. Proteolytic activity was detected in 87.10% of strains, while EPS and acetoin were produced by 29.03% and 48.39%, respectively. Lipolytic and amylolytic activities were generally weak. Notably, 74.19% of the strains exhibited antimicrobial activity, inhibiting at least one pathogen, with inhibition zones varying significantly (p < 0.0001).Dromedary camel milk from Algerian semi-arid regions represents a rich source of LAB strains with promising technological and antimicrobial potential. These native isolates could be further developed for use in additive-free fermented foods and natural biopreservation systems, supporting sustainable and functional food innovation.