ORIGINAL RESEARCH article
Front. Nutr.
Sec. Food Chemistry
This article is part of the Research TopicAntioxidant Activities and Induction Production Approaches (Physical, Chemical and Biotechnological) of Plant-based Bioactive NutrientsView all 4 articles
Preparation and bioactivities of selenium nanoparticles based on a polysaccharide from Dendrobium huoshanense
Provisionally accepted
- 1College of The Fifth Clinical Medical (Zhengzhou People's Hospital), Henan University of Chinese Medicine, Zhengzhou, China
- 2Guochen Technology (Henan) Group Co., Ltd., Zhengzhou, China
- 3Henan Agricultural University, Zhengzhou, China
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The present work reported the preparation and characterization of a polysaccharide (DPHs-1) from Dendrobium huoshanense and its influence on bioactivities of selenium nanoparticles (DPH-SeNPs). DPH-SeNPs were synthesized using DPHs-1 as stabilizer and dispersant. Experiments were conducted to investigate the effects of DHPs-1 concentration, temperature, the molar ratio of Vitamin C (Vc) to Sodium selenite (Na2SeO3), and reaction time on the preparation of DHP-SeNPs. Scanning electron microscopy (SEM) revealed that the DHP-SeNPs had a relatively rough and uneven surface. Transmission electron microscopy (TEM) imaging showed DHP-SeNPs were sphere-like in morphology and homogeneously distributed. X-ray diffraction (XRD) analysis indicated that the diffraction peak range changed, leading to the formation of a new substance. The fourier transform infrared spectroscopy (FT-IR) indicated that the main interaction between DHPs-1 and SeNPs occurred in the Se-O bonds, further leading to the stable spherical structure of DHPs-1 decorated SeNPs. Congo red test results revealed that the triple-helix structure of DHP-SeNPs was intact. DHP-SeNPs displayed favorable thermal stability. In vitro antioxidant experiment results showed that DHP-SeNPs had strong scavenging abilities for the 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl radical, and ABTS+ free radicals and they had good reducing power. The results of Cell Counting Kit-8 (CCK-8) experiments showed that both DHPs-1 and DHP-SeNPs with concentrations in the range of 5-320 μg/mL could promote cell proliferation without cytotoxicity. The results of scratch experiments showed that DHPs-1 and DHP-SeNPs, within concentrations of 20, 40, and 80 μg/mL, significantly promoted scratch healing of macrophage RAW264.7. Bioactivity tests indicated that DHPs-1 and DHP-SeNPs promoted the release of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interleukin-10 (IL-10) through upregulating the mRNA expression of TNF-α, IL-1, IL-6, and IL-10. In conclusion, DPH-SeNPs have novel bioactivities with promising applications in food and biomedicine.
Keywords: Dendrobium huoshanense polysaccharide, structural characterization, polysaccharide selenium nanoparticles, Antioxidant activities, immune activities
Received: 20 Aug 2025; Accepted: 17 Nov 2025.
Copyright: © 2025 Pei, Zhang, Xi, Gao, Ma and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Bingji Ma, mbj12345@henau.edu.cn
Li Wang, wanglihuina@163.com
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