Advancing In Vitro Cell Culture Practices: Achieving Truly Animal-Free Experiments and Scientifically Reliable and Reproducible Methods

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ORIGINAL RESEARCH

Accepted on 01 Oct 2025

Alternatives to animal-derived extracellular matrix hydrogels? An explorative study with HepaRG cells in animal-free hydrogels under static and dynamic culture conditions

Katharina Stefanie Nitsche
Iris Müller
Sophie Malcomber
Paul Lawford Carmichael
Hans Bouwmeester

doi 10.3389/ftox.2025.1649393

264 views

ORIGINAL RESEARCH

Published on 01 Oct 2025

Proliferation and metabolic activity of the Atlantic sturgeon cell line AOXlar7y under short-term serum-reduced conditions, and the effect of stimulation with growth factors and cytokines

Valeria Di Leonardo
Julia Brenmoehl
Heike Wanka
Bianka Grunow

doi 10.3389/ftox.2025.1636776

829 views

ORIGINAL RESEARCH

Published on 01 Oct 2025

Promoting ethical and reproducible cell culture: implementing animal-free alternatives to teaching in molecular and cell biology

Alexandra Nessar
Viola Röhrs
Mathias Ziersch
Ahmed S. M. Ali
Julia Moradi
Anke Kurreck
Johanna Berg
Jens Kurreck

doi 10.3389/ftox.2025.1670513

2,082 views

REVIEW

Published on 08 Aug 2025

Fetal bovine serum: how to leave it behind in the pursuit of more reliable science

Tilo Weber
Atena Malakpour-Permlid
Aline Chary
Vito D’Alessandro
Leah Haut
Sebastian Seufert
Esther Veronika Wenzel
James Hickman
Karen Bieback
3 more
Stina Oredsson

doi 10.3389/ftox.2025.1612903

4,521 views
2 citations

SYSTEMATIC REVIEW

Published on 27 Jun 2025

Towards the standardization of human platelet lysate production and its comparison to fetal bovine serum for human hematopoietic cell culture: a scoping review

Shamili Immalaraju
Srishti Goyal
Rukmini Jonnalagadda

doi 10.3389/ftox.2025.1496231

1,592 views
1 citation

MINI REVIEW

Published on 14 May 2025

Optimizing Wnt activation in fetal calf serum (FCS)-free organoid expansion media

Esther J. M. Liefting
Jeffrey J. Bajramovic

doi 10.3389/ftox.2025.1504469

1,490 views
1 citation

ORIGINAL RESEARCH

Published on 26 Mar 2025

Advancing humanized 3D tumor modeling using an open access xeno-free medium

Atena Malakpour-Permlid
Manuel Marcos Rodriguez
Kinga Zór
Anja Boisen
Stina Oredsson

doi 10.3389/ftox.2025.1529360

1,995 views
2 citations

PERSPECTIVE

Published on 06 Dec 2024

Considering future qualification for regulatory science in the early development of microphysiological systems: a case study of microthrombosis in a Vessel-on-Chip

Huub J. Weener
Heleen H. T. Middelkamp
Andries D. Van der Meer

doi 10.3389/ftox.2024.1513002

1,769 views
1 citation

ORIGINAL RESEARCH

Published on 22 Nov 2024

Increasing sustainability and reproducibility of in vitro toxicology applications: serum-free cultivation of HepG2 cells

Luisa Marie Pfeifer
Janike Sensbach
Frederic Pipp
Daniela Werkmann
Philip Hewitt

doi 10.3389/ftox.2024.1439031

3,087 views
4 citations

ORIGINAL RESEARCH

Published on 05 Nov 2024

Use of serum-free media for peripheral blood mononuclear cell culture and the impact on T and B cell readouts

Stella Cochrane
Ouarda Saib
David Sheffield

doi 10.3389/ftox.2024.1462688

4,484 views
2 citations

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Frontiers in Toxicology

In Vitro Toxicology
Regulatory Toxicology

Participating Journals

Frontiers in Cell and Developmental Biology
- 4.3 impact factor
- 11.4 citescore
Frontiers in Genetics
- Stem Cell Research
- 2.8 impact factor
- 6.2 citescore
Frontiers in Molecular Biosciences
- Cellular Biochemistry
- 4.0 impact factor
- 8.8 citescore

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Articles

Alternatives to animal-derived extracellular matrix hydrogels? An explorative study with HepaRG cells in animal-free hydrogels under static and dynamic culture conditions

Proliferation and metabolic activity of the Atlantic sturgeon cell line AOXlar7y under short-term serum-reduced conditions, and the effect of stimulation with growth factors and cytokines

Promoting ethical and reproducible cell culture: implementing animal-free alternatives to teaching in molecular and cell biology

Fetal bovine serum: how to leave it behind in the pursuit of more reliable science

Towards the standardization of human platelet lysate production and its comparison to fetal bovine serum for human hematopoietic cell culture: a scoping review

Optimizing Wnt activation in fetal calf serum (FCS)-free organoid expansion media

Advancing humanized 3D tumor modeling using an open access xeno-free medium

Considering future qualification for regulatory science in the early development of microphysiological systems: a case study of microthrombosis in a Vessel-on-Chip

Increasing sustainability and reproducibility of in vitro toxicology applications: serum-free cultivation of HepG2 cells

Use of serum-free media for peripheral blood mononuclear cell culture and the impact on T and B cell readouts

Frontiers in Toxicology

In Vitro Toxicology

Regulatory Toxicology

Participating Journals

Frontiers in Cell and Developmental Biology

Cell Growth and Division

Cellular Biochemistry

Stem Cell Research

Frontiers in Genetics

Stem Cell Research

Frontiers in Molecular Biosciences

Cellular Biochemistry