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Front. Phys. | doi: 10.3389/fphy.2019.00062

Spectral Phasor Analysis for Brillouin Microspectroscopy

  • 1Vienna Biocenter Core Facilities GmbH (VBCF), Austria

Brillouin Light Scattering (BLS) spectroscopy allows for the all-optical measurement of the hypersonic velocities in a sample, from which one can extract high-frequency elastic moduli. Recent advances in high resolution optical imaging spectrometers have made it conducive to studying live biological samples and further its implementation as an imaging modality for the life sciences. One major challenge in this context is the relatively weak BLS signal together with the subtle BLS spectral variations observed in many biological samples. Here we show that using spectral phasor analysis one can more easily distinguish variations in noisy spectra compared to standard least-squares (LS) fitting. There is no fitting in phasor analysis, and it is both robust in regards to unaccounted for functional variations in the spectra, and orders of magnitude faster than LS-fitting. As such it can prove particularly useful for increasing contrast in Brillouin imaging as well as for high-throughput BLS applications such as cell-sorting and medical diagnostics especially for statistically compromised or noisy data.

Keywords: Brillouin light scattering (BLS), Brillouin microscopy, Optical microscopy, optical spectroscopy, Phasor analysis, Spectral Phasor Analysis

Received: 06 Mar 2019; Accepted: 08 Apr 2019.

Edited by:

Stefan G. Stanciu, Politehnica University of Bucharest, Romania

Reviewed by:

Zhaokai Meng, Cytochip Inc., United States
Jitao Zhang, University of Maryland, College Park, United States  

Copyright: © 2019 Elsayad. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Kareem Elsayad, Vienna Biocenter Core Facilities GmbH (VBCF), Vienna, Austria, kareem.elsayad@vbcf.ac.at