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Frontiers in Fungal Virus Research

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Front. Plant Sci. | doi: 10.3389/fpls.2018.01738

Diverse, novel Mycoviruses from the Virome of a hypovirulent Sclerotium rolfsii strain

Jun Zi Zhu1, Hong Jian Zhu1, Bi Da Gao1,  Qian Zhou1* and  Jie Zhong1*
  • 1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (CAAS), China

Sclerotium rolfsii, which causes southern blight in a wide variety of crops, is a devastating plant pathogen worldwide. Mycoviruses that induce hypovirulence in phytopathogenic fungi are potential biological control resources against fungal plant diseases. However, in S. rolfsii, mycoviruses are rarely reported. In a previous study, we found a hypovirulent strain carrying a diverse pattern of dsRNAs. Here, we utilized the RNA_Seq technique to detect viral sequences. Deep sequencing, RT-PCR and Sanger sequencing validation analyses revealed that this strain harbors various new viral species that show affinity to the distinctly established and proposed families Benyviridae, Endornaviridae, Fusariviridae, Hypoviridae, and Fusagraviridae. Moreover, some viral sequences that could not be assigned to any of the existing families or orders were also identified and showed similarities to the Alphavirus, Ourmiavirus, phlegivirus-like and Curvularia thermal tolerance virus-like groups. In addition, we also conducted deep sequencing analysis of small RNAs in the virus-infecting fugal strain. The results indicated that the Dicer-mediated gene silencing mechanism was present in S. rolfsii. This is the first report of viral diversity in a single S. rolfsii fungal strain, and the results presented herein might provide insight into the taxonomy and evolution of mycoviruses and be useful for the exploration of mycoviruses as biocontrol agents.

Keywords: Mycovirus, Sclerotium rolfsii, virome, biocontrol, Deep sequencing

Received: 31 Aug 2018; Accepted: 08 Nov 2018.

Edited by:

Jiatao Xie, Huazhong Agricultural University, China

Reviewed by:

Sotaro Chiba, Asian Satellite Campuses Institute, Nagoya University, Japan
Shinyi Lee Marzano, University of Illinois at Urbana-Champaign, United States  

Copyright: © 2018 Zhu, Zhu, Gao, Zhou and Zhong. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Mrs. Qian Zhou, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (CAAS), Beijing, Hunan Province, China, zhouqian2617@hunau.edu.cn
Mr. Jie Zhong, State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (CAAS), Beijing, Hunan Province, China, 1601389330@qq.com