ORIGINAL RESEARCH article
Front. Chem.
Sec. Analytical Chemistry
This article is part of the Research TopicEmerging Nanocomposites in Electrochemical SensorsView all articles
Construction of the indicator-free electrochemical biosensor with magnetically self-assembly based on Fe3O4/α-Fe2O3 magnetic heterogeneous nanorods for the ultra-sensitive detection of CYFRA 21-1 DNA
Provisionally accepted
- 1Zhenjiang 359 Hospital, Zhenjiang, China
- 2The People’s Hospital of Danyang, Affiliated Danyang Hospital of Nantong University, Zhenjiang, China
- 3Panzhihua University, Panzhihua, China
- 4zhucheng maternal and child health hospital, Zhucheng, China
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Lung cancer prevalence rate has been rising steadily in recent years, for the prevention and treatment, the detection of tumor marker CYFRA 21-1 DNA demonstrates its significance. In this work, an electrochemical biosensor was constructed for sensitive detection of CYFRA 21-1 DNA based on the novel developed Fe3O4/α-Fe2O3 magnetic heterogeneous nanorods (MHNRs). Firstly, Fe3O4/α-Fe2O3 MHNRs were prepared by hydrothermal-calcination method, and then Fe3O4/α-Fe2O3@Au magnetic nanocomposites (MNCs) were obtained though gold-coating. Subsequently, the magnetic self-assembling electrochemical biosensor based on Fe3O4/α-Fe2O3@Au MNCs was successfully constructed, which was verified by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). To optimize the biosensor's experimental conditions and evaluate its performance, differential pulse voltammetry (DPV) was conducted. The results showed that the detection range of CYFRA 21-1 DNA was 10 pM-10 μM, the limit of detection (LOD) was 1.5 pM. The biosensor exhibited excellent selectivity, reproducibility, and stability; the relative standard deviation (RSD) was 2.01%. The average recovery rate in the spiked diluted human serum samples was 101.4%, and the RSD was ≤ 5.2%, indicating that the biosensor possessed promising prospect.
Keywords: aptamer, CYFRA 21-1 DNA, detection, electrochemical biosensor, Fe3O4/α-Fe2O3@Au magneticnanocomposites
Received: 01 Sep 2025; Accepted: 04 Dec 2025.
Copyright: © 2025 Yi, Deng, Wang, Wang, Wang and Wu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Hongfei Wang
Zhan-ao Wu
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