ORIGINAL RESEARCH article
Front. Mater.
Sec. Biomaterials and Bio-Inspired Materials
Volume 12 - 2025 | doi: 10.3389/fmats.2025.1568591
Preparation of SDF-1α-loaded electrospun coaxial microspheres and their study on promoting migration and differentiation of dental pulp stem cells
Provisionally accepted
- 1Binzhou Medical University, Binzhou, Shandong Province, China
- 2Qingdao Stomatological Hospital, Qingdao, China
Select one of your emails
You have multiple emails registered with Frontiers:
Notify me on publication
Please enter your email address:
If you already have an account, please login
You don't have a Frontiers account ? You can register here
The most common treatment for pulpitis or pulp necrosis is root canal therapy (RCT). However, in immature permanent teeth, the loss of pulp vitality impedes root development and apical closure, resulting in thin and fragile dentin walls. Consequently, extensive research has focused on regenerative endodontic treatment (RET) to restore tooth vitality and promote root development. Dental stem cells (DPSCs), scaffolds, and growth factors are crucial components of RET. Stromal Cell-Derived Factor-1α (SDF-1α) has recently been shown to enhance odontogenic differentiation in human dental pulp cells (hDPSCs) and plays a key role in recruiting, migrating, and differentiating various stem/progenitor cells, including hematopoietic and mesenchymal stem cells, and its bioactivity must be protected during drug delivery to be effective. Based on the above findings, we employed electrospinning technology to fabricate SDF-1α/Poly(lactic-co-glycolic acid) (PLGA) microspheres with a core-shell structure for enhancing scaffolds in pulp regeneration. The results indicate that these core-shell drug-loaded microspheres have a uniform particle size and exhibit excellent biocompatibility, as well as favorable sustained-release properties. Notably, the growth factors released by the microspheres in the two weeks continue to promote the migration of hDPSCs, demonstrating that the growth factors retained high activity during this period. This ensures the long-term efficacy of the drug and optimizes drug delivery in RET. Moreover, these microspheres are capable of continuously promoting the differentiation of hDPSCs into odontogenic and angiogenic lineages without the need for repeated drug administration, indicating their potential application in pulp regeneration therapy.
Keywords: SDF-1α, microspheres, sustained release, Dental pulp regeneration, Human dental pulp stem cells
Received: 30 Jan 2025; Accepted: 13 Jun 2025.
Copyright: © 2025 Feng, Li, An, Shi, Wang, Lu and Sun. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Degang Sun, Qingdao Stomatological Hospital, Qingdao, China
Disclaimer: All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.