ORIGINAL RESEARCH article
Front. Nutr.
Sec. Food Chemistry
This article is part of the Research TopicExtracts From Plants and Other Natural Sources: Application, Characterization, Optimization, and Their Use - Volume IIView all 9 articles
Isolation, Purification, and Characterization of Polysaccharides from Dendrobium findlayanum: Biological Activities and Po-tential Therapeutic Applications
Provisionally accepted
Ji Chen1*
Lin-Hong Li2Xu Mo1Li-Juan Wu1Chao-Wen Zhang1Yu Sun1Yao Liu1Rui Li1
Yuan-Feng Zou1Yan Zheng3Fan Liu1
Mengliang Tian1*- 1Sichuan Agricultural University - Chengdu Campus, Chengdu, China
- 2Leshan Academy of Agricultural Sciences, Leshan, China
- 3Department of Medical Plastic and Cosmetic, The Third People's Hospital of Chengdu (The Affiliated Hospital of Southwest Jiaotong University), College of Medicine, Southwest Jiaotong University, Chengdu, China
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The crude polysaccharides of Dendrobium findlayanum were extracted by ethanol precipitation and separated using DEAE Sepharose Fast Flow and Sepharose 6 Fast Flow chromatography to obtain DFP-NP and its purified fraction DFP-NP1. Structural characterization revealed that DFP-NP (12.3 kDa) primarily consisted of mannose, rhamnose, glucose, and galactose in a molar ratio of 11.61:0.68:84.78:2.93, with FT-IR spectra confirming typical polysaccharide functional groups. In vitro assays demonstrated that DFP-NP1 polysaccharides at concentrations of 5.0-20 μg/mL enhanced CAT and SOD activities and upregulated related gene expression in IPEC-J2 cells, while reducing TNF-α, IL-1β, and IL-6 secretion. They decreased expression levels of genes associated with the antioxidant signaling and cellular inflammatory pathways in IPEC-J2 cells. Therefore, D. findlayanum polysaccharides possess significant potential for application as a nutraceutical supplement to alleviate intestinal inflammation linked to oxidative stress, leveraging a dietary approach for health maintenance.
Keywords: Dendrobium findlayanum polysaccharides, Inflammation, Intestinal porcine enterocyte cells, Oxidative Stress, Structural characterisation
Received: 06 Nov 2025; Accepted: 30 Nov 2025.
Copyright: © 2025 Chen, Li, Mo, Wu, Zhang, Sun, Liu, Li, Zou, Zheng, Liu and Tian. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Ji Chen
Mengliang Tian
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