Tantalum Nanoparticles Reinforced PCL Scaffolds Using Direct 3D Printing for Bone Tissue Engineering
Zixuan Xiong
Wenbin Liu
Hu Qian1,2 
Pengfei Lei- 1Department of Orthopedics, Xiangya Hospital of Central South University, Changsha, China
- 2Hunan Engineering Research Center of Biomedical Metal and Ceramic Implants, Changsha, China
- 3Xiangya School of Medicine, Central South University, Changsha, China
Polycarbonate (PCL) has been widely used in tissue engineering, but its hydrophobicity and low biological activity limit its further promotion and application. By adding nanoparticles, the hydrophilicity and biological activity of PCL can be improved. In this study, different amounts of Ta (1–10%wt) were added to PCL, and then their mechanical and biological properties were studied in vitro. XRD found that 5%Ta-PCL has the highest crystallinity. At the same time, cell experiments CCK8, cell adhesion, osteogenic differentiation, and osteogenesis related gene expression showed that Ta can enhance the mechanical and biological properties of PCL, while 5% Ta-PCL showed the best mechanical and biological properties. This composite of tantalum and PCL could have a clinical potential for orthopedic implants.
Introduction
Every year, millions of people suffer from massive bone defects due to various reasons, such as tumors, trauma, and infections, resulting in an increasing clinical demand for bone defect implants (Bhattacharjee et al., 2017; Wang and Yeung, 2017). Autologous bone transplantation is the golden standard for clinical treatment of bone defects, but its source is limited, and it will increase the risk of fracture at the donor site, which greatly limits its clinical application in the treatment of large bone defects (Tang et al., 2016; Ji et al., 2020). Xenogeneic bone can also be used to treat large bone defects, but there is a risk of immune response, disease transmission and treatment safety (Ma et al., 2018). Therefore, scaffold-based tissue engineering technology has attracted more and more people’s interest.
Polycarbonate (PCL) is a biodegradable polyester certified by the FDA (Fedorová et al., 2015; Felice et al., 2018). Which is non-toxic and harmless to tissues and has good biocompatibility. It is often used as a scaffold in bone tissue engineering (Masoudi Rad et al., 2017; Wang et al., 2018; Ji et al., 2020). At the same time, the degradation products, CO2, and H2O, are no harmful substances (Bittner et al., 2019). However, the hydrophobic surface together with the lack of its strength and biological activity result in the unfavorable properties of PCL for cell adhesion (Heo et al., 2019). Therefore, how to improve the strength and hydrophilicity of PCL is a hot spot in current research.
As a transition metal element, tantalum has good physical and chemical properties such as high melting point, high strength, wear resistance and corrosion resistance (Niinomi et al., 2012; Torstrick et al., 2016). Among them, the good corrosion resistance is due to the Ta2O5 film formed on the surface. Tantalum is a metal material with excellent biocompatibility, whose porous structure can mimic the trabecular bone structure of normal bone tissue (Levine et al., 2006). At the same time, tantalum and nano-tantalum have also been proved to have the characteristics of promoting the osteogenic differentiation of mesenchymal stem cells (Mohandas et al., 2014; Wang et al., 2015; Kang et al., 2017). Nano-tantalum have a larger specific surface area than tantalum metal which can provide stronger biological activity. Zhu Hao et al. used tantalum nanoparticles combined with PEEK had been shown to enhance the mechanical properties and osteogenic activity of PEEK (Zhu et al., 2019). Park et al. demonstrated Ta-implanted PLA could significantly enhanced osseointegration and osteogenesis compared with bare PLA (Park et al., 2019). Since tantalum nanoparticles are stable and biocompatible, they might strengthen the properties of the hydrophilic and mechanical of PCL, and potentially alter the properties of PCL for better prospect in orthopedic use.
In this study, we first used nano-tantalum blended PCL with 3D printing to enhance PCL, and fabricated Ta-PCL doped with different proportions of tantalum nanoparticles (1, 5 and10%wt) to study the effect of the 3D Ta-PCL scaffold on the osteogenic differentiation of mesenchymal stem cells in vitro. Our hypothesis is that Ta nanoparticles can enhance the crystallinity and hydrophilic properties of PCL (Supplementary Figure S3) and further enhance the osteogenic differentiation ability of mesenchymal stem cells. The osteogenic differentiation ability of 3D Ta-PCL was tested by RT-PCR and COL I staining.
Materials and Methods
Sample Fabrication and Preparation
The Polycarbonate particles (Sigma, MW=80,000, United Kingdom) are thoroughly mixed with tantalum nanoparticles (Dk Nano Technology Co., LTD., Beijing, China Size = 50 nm) (Supplementary Figure S2), and the mixture was added to a solution composed by dichloromethane and absolute ethanol. The proportion of tantalum nanoparticles is controlled at 1, 5 and 10wt%. The volume ratio of dichloromethane to absolute ethanol is controlled at 2:3. Pure PCL particles were used as a control group. For each group, according to a specific procedure, a mechanical extrusion method is used to form the material. In short, the mixed material is first added to the mixed solution, and then extruded through a fine pinhole to quickly evaporate the solvent, leaving only the solid powder to form the stent (Figure 1A).
FIGURE 1. The characterization of Ta/PCL scaffold. (A) The synthesis process of Ta/PCL. (B) The general view of Ta/PCL solution. (C) Front view of the structure of Ta/PCL.
We designed several programs in advance. Small square samples (10 × 10 × 0.3 mm3) were used in 24-well culture plates for surface characterization and in vitro studies. Large square samples (13 × 13 × 0.3 mm3) were used for RNA expression related tests. In order to remove the debris on the samples and the microorganisms in the air stained during printing, all samples were soaked in medical ethanol for 8 h and then irradiated with ultraviolet light for 1 h. Then all samples were washed 5 times with phosphate buffered saline (PBS).
Material Properties
The surface morphology was examined by scanning electron microscope (Quanta-200, United States) after sputtered with 5 nm of platinum coating (Gressington 308R,United Kingdom). The images were taken in different magnifications when the acceleration voltage is 10 kV, after that, the energy-dispersive X-ray spectroscopy (EDS) was performed.
In vitro Studies
Cell Culturing
The bone marrow mesenchymal stem cells of rats (rBMSCs) are used for in vitro research (Song et al., 2017). The cells were cultured in DMEM/F-12 essential medium (Gibco, United States) in standard culture environment, such as 37°C humid environment and 5% CO2. The medium contains 10% fetal bovine serum and 1% antibiotics (50 IU penicillin/ml and 50 μg streptomycin/ml) and it was changed every three days, the cells were passaged when the coverage of the medium reached 90%.
Cell Adhesion
The rBMSCs were seeded onto the surface of the samples (10 × 10 × 0.3 mm3) at a density of 1 × 104/well. After culturing for 24 h respectively, the medium was removed and samples were rinsed with PBS for 3 times, together with the fixation with 4% paraformaldehyde in PBS. Samples were then stained with phalloidin and DAPI. Images were taken under confocal scanning microscopy (Leica TCS SP8 X, Germany). For SEM characterization, the samples were dehydrated by gradient ethanol and dried in the air.
Osteogenesis Related Gene Expression
The expression of osteogenesis-related genes was analyzed by real-time polymerase chain reaction (qPCR). Briefly, rBMSCs were seeded with a density of 2 × 104/well and cultured for 7 days, Total RNA was extracted using Trizol reagent (Invitrogen, United States), Subsequently, nanodrop 2000 was used to obtain the concentration of RNA, after which the reverse transcription using a ReverTra Ace® qPCR RT Kit (Toyobo, Japan). The experiment was carried with CFX Connect Real-Time PCR Detection System (BIO-RAD,United States)，β-actin was used as a housekeeping gene, and the relative quantification of mRNA (ALP, OCN and COL I) was determined with 2 -△△Ct method. The primers are ALP: Forward primer sequence (5′-3′): GCACAACATCAAGGACATCG; Reverse primer sequence (5′-3′): TCAGTTCTGTTCTTGGGGTACAT; COL I: Forward primer sequence (5′-3′): GAAGACCTGGCGAGAGAGGA; Reverse primer sequence (5′-3′): TCAATCCATCCAGACCGTTG. OCN: Forward primer sequence (5′-3′): GCCCTGACTGCATTCTGCCTCT; Reverse primer sequence (5′-3′): TCACCACCTTACTGCCCTCCTG.
Statistical Analysis
Data were expressed as means standard deviations (SD) from experiments performed in quintuplicate. Statistical analysis was performed using the one-way analysis of variance (ANOVA) with Tukey-Kramer Multiple comparison post-test using GraphPad Instant Software (GraphPad Prism Software, Inc. United States) with p < 0.05 being considered to be statistically significant.
Result and Discussion
Characterization of Ta-PCL Scaffolds
We first aimed to obtain the Ta-PCL inks with perfect Printability, and then 3D printed different proportions of TA-PCL onto a glass slide through an extrusion printer. It could be seen from the general view (Figures 1B, C) that the printed shape of 1, 5 and 10wt% Ta-PCL can be formed regularly. With the increasing proportions of Ta nanoparticles, the color of the scaffolds gradually became black. The pore size and porosity of the scaffold are important influencing factors for bone tissue regeneration. A proper pore size can promote the formation of blood vessels and the flow of nutrients to promote bone regeneration. The pore size of the scaffold was analyzed. The pore size of the PCL scaffold was 450 ± 10 um, 1%Ta -PCL scaffold pore diameter is 460 ± 10 um, 5%Ta-PCL scaffold pore diameter was 430 ± 10 um, 10%Ta-PCL pore diameter was 440 ± 10 um.300–500 um is the best pore diameter to promote osteogenesis, and all scaffolds’ pore were designed to be in this range (Ma et al., 2020), the size of the pore changes slightly between different scaffold which may related to the error of the printer. At the same time, the tantalum nanoparticles were found uniformly dispersed in the PCL through the SEM rather than agglomerated together (Supplementary Figure S1), which is basically consistent with the researches of others. To further confirm the presence of Ta nanoparticles in the scaffold, EDS was performed. The EDS results show that there are characteristic peaks of tantalum on the surface of PCL doped with different contents of tantalum.
In order to further explored the crystallization situation inside the Ta-PCL, XRD was performed. Figure 2A shows that the XRD patterns of Ta-PCL scaffolds and pure PCL scaffolds with different contents. In the XRD pattern, Ta shown three typical characteristic peaks at 2θ = 38.2°, 55.1° and 69.1°. The corresponding crystal planes were (110) (200) and (211), which were similar to the diffraction peaks of standard Ta. From the XRD pattern, pure PCL fibers shown two typical diffraction peaks at 2θ = 21.4° and 24.3°. In the composite scaffolds, with the increase of Ta content, the diffraction intensity of the PCL characteristic peak first increased and then decreased, indicating that the crystallinity of PCL was improved after the usage of Ta, and the crystallinity of Ta-PCL scaffold reached the summit at 5wt%. That is because of The non-crystalline region of PCL is made up of the polymer chain segment, and the nanoparticles and the chain segment will work together to reduce the polymer base’s free body product, allowing the chain to be arranged in the non-crystalline zone, so the crystallization increase, and when the amount of nanoparticles increases, it also damage the chain failure of the original crystallization zone and the crystallization degree decreases. Based on the results above, we had successfully prepared 3D printed Ta-PCL scaffold with different contents.
FIGURE 2. Physico-chemical characterization of Ta/PCL scaffold. (A) XRD spectra. (B) EDS spectra. The arrows represent tantalum nanoparticles.
Cell Compatibility of the Ta-PCL Scaffold
In order to evaluate the biocompatibility of the scaffold, we conducted CCK8 and Live/dead staining to evaluate the cytotoxicity of the scaffold. As was shown in Figure 3, the addition of Ta nanoparticles could promote the proliferation of mesenchymal stem cells, and with the increasing content of tantalum, 10% Ta-PCL shown to be the best cell proliferation rate. Live/dead staining also shown that PCL, 1, 5 and 10% Ta-PCL were all non-toxic and biocompatible.
FIGURE 3. Cell viability of Ta/PCL scaffold. (A) Live/Dead assay of MSCs cultured in the scaffold after 3 days. (B) The CCK8 assay of MSCs when cultured with Ta/PCL after 1 day, 3 days and 7 days *p < 0.05 vs PCL.
Initial cell adhesion and spreading on biomaterials, the first phase of the cell material interaction, is a key step in the regulation of the cell proliferation and differentiation that subsequently follows. In order to evaluate the adhesion performance of Ta-PCL to mesenchymal stem cells, they were planted on the scaffold for 3 days Figure 4 showed that PCL had poor surface cell morphology due to its poor hydrophilicity. The cell morphology on 1% Ta-PCL was more stretched, while the cells on 5 and 10% Ta-PCL were fully stretched with a large number of pseudopods being observed, while 5% Ta-PCL has more stretched cell morphology and better cell adhesion performance.
FIGURE 4. Cell adhesion of Ta/PCL scaffold. Cytoskeleton of MSCs cultured on the scaffold for 5 days.
Osteogenesis of the Ta-PCL Scaffold
Col I expression in BMSCs for calcium nodules produced by mineralization in the late stage of osteogenic differentiation, which may further promote the mineralization in the later stage of osteogenic differentiation (Wang et al., 2021). Co-cultivation of mesenchymal stem cells with 3D printed Ta-PCL scaffolds 7 days later, Figure 5A showed that the COL I expression of the Ta-PCL scaffold was higher than that of the PCL scaffold alone, indicating that the addition of Ta can promote the secretion of collagen, which may further promote the formation of calcium nodules. 5%Ta-PCL had the highest expression of COL I, followed by 10 and 1%Ta-PCL. This was basically consistent with the research of others.
FIGURE 5. Osteogenesis of Ta/PCL (A) COL I staining. (B) Osteogenesis related gene expression analyzed by qPCR. *p < 0.05 vs. PCL; #p < 0.05 vs. 1%Ta-PCL; & p < 0.05 vs. 5% Ta-PCL.
To further examined the influence of the Ta-PCL on the differentiation of osteogenic at the molecular level, The expression of osteogenic marker genes such as collagen I (Col I), alkaline phosphatase (ALP), osteocalcin (OCN) and in BMSCs was assayed using quantitative real-time PCR after 7 days of osteogenic induction culture. As is shown in Figure 5B,.ALP and COL I are characteristic markers of osteogenic differentiation, which can promote cell osteogenic mineralization and the formation of calcium nodules (Chen et al., 2020; Shen et al., 2020). Comparing with PCL, 5% Ta-PCL shown the highest ALP expression. It also had the highest COL I expression, which was perfectly consistent with immunofluorescence staining (Figure 5A). OCN is a characteristic marker of late osteogenesis (Rasi Ghaemi et al., 2016; Sun et al., 2019). In Figure 5B, tantalum nanoparticle could enhance the expression of OCN compared to PCL, with the 5%Ta-PCL having the highest gene expression compared to 1 and 10%Ta-PCL. Generally speaking, mesenchymal stem cells had the highest osteogenic differentiation potential in 5% Ta-PCL in terms of gene expression and protein expression.
Conclusion
In summary, we obtained a scaffold with good pore structure and porosity by 3D printing the mixture of PCL and Ta nanoparticles. SEM and XRD showed that Ta nanoparticles were evenly distributed in PCL. The 5% Ta-PCL composite scaffold had the best crystallinity. At the same time, CCK8 and live/dead staining found that the addition of Ta nanoparticles could promote the proliferation and osteogenic differentiation of mesenchymal stem cells while it had nothing to do with the activities of cells. In addition, RT-PCR found that 5% Ta-PCL has the best osteoinductive property compared to 1 and 10% Ta-PCL. Altogether, these results shown that the 3D printed Ta-PCL scaffold has a good application prospect in bone tissue engineering.
Data Availability Statement
The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors.
Ethics Statement
The animal study was reviewed and approved by the Ethical Review Board at the Xiangya Hospital of Central South University (Changsha, China).
Author Contributions
The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript. LW and XZ contributed equally.
Funding
This study was supported by the Natural Science Foundation of Hunan Province, China (Grant No. 2018JJ3844 and 2019JJ40499), the Scientific Research Project of Health and Family Planning Commission of Hunan Province, China (Grant No. B2019188), the Science and technology Innovation Leading Project for High-tech Industry of Hunan Province (Grant No. 2020SK2008), the Major science and technology projects of Changsha City (Grant No. kh2003016), the Young Science Foundation of Xiangya Hospital Central South University (Grant No. 2017Q07), the Postdoctoral Research Program of Xiangya Hospital Central South University (Grant No. 223551). National key research and development project (2016YFC1100605 and 2018YFB1105504), Natural Science Foundation of China (Grant No. 82002277 and 81672656). Project Program of National Clinical Research Center for Geriatric Disorders (Xiangya Hospital, Grant No. 2020LNJJ15).
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmats.2021.609779/full#supplementary-material.
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Keywords: polycarbonate(PCL), tantalum, bone tissue engineering, osteogenesis, 3D printing
Citation: Xiong Z, Liu W, Qian H, Lei T, He X, Hu Y and Lei P (2021) Tantalum Nanoparticles Reinforced PCL Scaffolds Using Direct 3D Printing for Bone Tissue Engineering. Front. Mater. 8:609779. doi: 10.3389/fmats.2021.609779
Received: 25 September 2020; Accepted: 07 January 2021;
Published: 23 February 2021.
Edited by:
Nan Kang, Northwestern Polytechnical University, ChinaReviewed by:
Mehdi Derradji, Polytechnic School of Algiers, AlgeriaYuanqing Li, Chongqing University, China
Copyright © 2021 Xiong, Liu, Qian, Lei, He, Hu and Lei. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Pengfei Lei, pengfeilei@csu.edu.cn; Yihe Hu, csuhuyihe@163.com
†These authors have contributed equally to this work