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Front. Mater., 08 December 2021
Sec. Biomaterials
Volume 8 - 2021 |

Preliminary Studies for One-Step Fabrication of Metallic Iron-Based Coatings on Magnesium as Temporary Protection in Biodegradable Medical Application

www.frontiersin.orgYuyun Yang1,2,3, www.frontiersin.orgZizhong Shi1, www.frontiersin.orgXiufang Cui1*, www.frontiersin.orgYuejun Liu1, www.frontiersin.orgGuo Jin1, www.frontiersin.orgSannakaisa Virtanen3, www.frontiersin.orgAldo R. Boccaccini2 and www.frontiersin.orgPeng She4*
  • 1Department of Material Science and Chemical Engineering, Institute of Surface/Interface Science and Technology, Harbin Engineering University, Harbin, China
  • 2Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany
  • 3Department of Materials Science and Engineering, Institute for Surface Science and Corrosion, University of Erlangen-Nuremberg, Erlangen, Germany
  • 4Department of Orthopaedics, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, China

Iron and magnesium are being considered as promising candidates for biodegradable materials in medical applications, both materials having their specific advantages and challenges. A hybrid of metallic iron and magnesium in a layered composite is studied in the present work, to combine the merits of both metals. A single-step dip-coating method was employed to prepare the layered composite material. Morphology, composition, crystal structure and corrosion behavior of the Mg/Fe sheet were assessed by SEM, EDX, XRD, and electrochemical measurements. The Mg/Fe layered composite sheet is composed of the magnesium substrate, a 1–2 µm metallic iron coating, and a pompon-like Mg(OH)2/MgO top layer. Long-term open-circuit potential measurements revealed that the Mg/Fe sheet samples exhibit a “self-healing” effect in Dulbecco’s modified Eagle’s medium.

1 Introduction

Since Lane introduced the application of metal plates for bone fracture fixation 100 years ago, metallic biomaterials have achieved huge development for a variety of biomedical applications (Hornberger et al., 2012; Mao et al., 2017; Li et al., 2019). The most well-known metallic biomaterials are high corrosion-resistant materials, such as 316 stainless steel (Tan et al., 2003; Shih et al., 2004; Patnaik et al., 2020), cobalt-chromium alloys (Ducheyne and Healy, 1988; Que and Topoleski, 2000; Kereiakes et al., 2003; Watanabe et al., 2021), titanium and its alloys (Helary et al., 2009; Huang et al., 2010; Niinomi et al., 2012; Jin et al., 2015; Lourenço et al., 2020), tantalum alloys (Miyazaki et al., 2000; Shimko et al., 2005; Zhou et al., 2007; Rodriguez-Contreras et al., 2021), as well as precious alloys (O’Brien, 1997; Chen et al., 2005; Niinomi et al., 2015). Another group of metals consists of biodegradable materials, such as pure iron and a variety of Fe-based alloys as well as pure magnesium and Mg alloys (Wu et al., 2016; Mao et al., 2017; Yang et al., 2018; Gorejová et al., 2019; Costantino et al., 2020; Zhu et al., 2021).

In the meantime, elements such as Fe and Mg are also considered as essential trace elements participating in a wide variety of metabolic processes, such as enzyme catalysis, oxygen transport, energy metabolism, and DNA and RNA synthesis (Zhang et al., 2018; Lee et al., 2021). Released Fe and Mg ions during biodegradation reactions directly incorporate into the metabolic processes, affecting the metabolic functions and the biodegradation of metallic implants.

Compared with non-degradable materials, biodegradable metals should not cause permanent physical irritation and they avoid the need to remove a temporary implant in a second operation (Schinhammer et al., 2013; Mao et al., 2017). In principle, a controllable degradation rate should be achieved as this is a key requirement for degradable metallic biomaterials. Regarding degradation, Mg (Mg alloys) and Fe (Fe alloys) present different challenges. For example, the high dissolution rate of magnesium in biological environments largely limits its biomedical application (Fischerauer et al., 2013). Therefore, various surface modification and coating methods (Rajabalizadeh and Seifzadeh, 2014; Seifzadeh and Farhoudi, 2016; Nezamdoust et al., 2019; Abdi-Alghanab et al., 2020; Ouyang et al., 2020; Chen et al., 2021) are applied to tailor the undesired fast degradation rate into a moderate one to fulfill the therapeutic tasks. For instance, Zhu (Zhu et al., 2021) prepared a poly (d,l-lactic acid)-coated magnesium alloy-based rapamycin drug-eluting bioresorbable stent. In vivo and in vitro studies showed that the polymeric coatings provided a suitable degradation rate of magnesium alloy stents.

Compared with Mg-based alloys, pure iron and its alloys exhibit better mechanical properties and cause no excessive hydrogen evolution during degradation. On the contrary, several studies have been performed to increase the degradation rate of Fe-based materials for the possible application in temporary implants, as Fe-based materials show a too slow corrosion rate in biological environments (Abou Neel et al., 2005; Moravej et al., 2010; Zhang et al., 2010; Francis et al., 2015; Gorejová et al., 2019; Lee et al., 2021). For example in the research of Lee et al. (Lee et al., 2021) a novel combination of nanostructured surface topography and galvanic reaction was reported to achieve a uniform and accelerated degradation of a Fe implant.

Based on related research work on tailoring the degradation behavior of both biodegradable Mg materials (to decrease degradation) and Fe materials (to increase degradation), the idea of combining Mg and Fe together, especially by using iron coating as temporary protection of Mg substrates, is a feasible approach. In this design, two biocompatible metallic materials are introduced in one system, which greatly enhances the biocompatibility of the combined material for further investigation. On the other hand, the metallic iron serving as temporary protection on Mg would achieve the goal of tailoring the degradation rate of both Mg and Fe at the same time. Metallic iron coatings could protect magnesium substrates from aggressive corrosion attacks in the physiological environment until the metallic iron coatings are fully biodegraded. Meanwhile, by comparing with the degradation performance of bulk Fe materials, the degradation behavior of Fe coatings will be intensively affected by their thickness and structure.

Hence, in this study, metallic iron coatings are produced on magnesium substrates by a simple single-step dip-coating method. The reactions taking place in an aqueous solution are spontaneously driven by the electrochemical properties of the participating elements Mg and Fe, and no external energy was introduced during the process (Yan and Xue, 2006). This approach is in contrast to previous attempts to produce metallic coatings via several deposition ways, such as high velocity oxygen fuel, plasma spraying, physical vapor deposition, laser cladding, sol-gel, and kinetic spraying (Livsey, 1981; Liu et al., 2009; Nezamdoust and Seifzadeh, 2017; Dayani et al., 2018; Ferrández-Montero et al., 2019; Lin et al., 2021; Akhter et al., 2022). In most of these methods, fine powders as feedstock and a complex apparatus are needed to fabricate the metallic coatings on the substrate. The here explored one-step dip-coating method combines biodegradable metallic magnesium and iron together in one composite, and the different degradation behaviors of the two metals are exploited. The principle of the coating process is the immersion plating of Fe, taking advantage of the difference in the electrochemical potentials of the substrate and the coating material.

2 Experimental

2.1 Materials Preparation

Commercially pure magnesium rod of 25.4 mm diameter and 99.9% purity, (Chempur Feinchemikalien und Forschungsbedarf GmbH, Germany) was cut into 2–4 mm thickness slices to be used as substrates. A 1200 grit Microcut fleece (Buehler GmbH, Germany) was used for grinding with an ethanol/glycerol (3:1) mixture as lubricant. After that, the samples were cleaned in ultra-sonic bath in ethanol for 3 min. Subsequently, the samples were polished in three steps with diamond paste (6 µm, 3 µm, 1 µm) and a lubricant which consisted of distilled water/ethanol/neutral soap (1,000 ml:250 ml:10 g), followed by ultrasonic cleaning immediately after each polishing step. All samples were rinsed with ethanol and dried with hot air stream.

The fabrication of the layered composite coating was carried out by a dip-coating method. According to the electrochemical series of the elements, a metal cation in an electrolyte can be reduced by any metal with a lower electrochemical potential. In this study, a less noble Mg substrate was applied to reduce Fe3+ into Fe and, as a consequence, Mg was oxidized into Mg2+ during the dipping process. The chemical reaction is according to Eq. 1.


For this process, 1 g iron (III) chloride hexahydrate (Sigma-Aldrich) was completely dissolved in 20 ml de-ionized water under magnetic stirring for 5 min. The Mg substrates were immersed in the coating solution. The dip coating procedure was carried out at room temperature for 90 s. Immediately after being taken out from the suspension, the samples were rinsed in ethanol for 20 s and dried with hot air stream. As shown in Figure 1, mirror-like polished magnesium samples were completely covered by a brown composite coating after the treatment. A thin outer oxidized iron layer formed after taking the samples out from the suspension which could contribute to the brown color.


FIGURE 1. Evidence of the change of the Mg sample surface upon treatment in Fe-bearing coating solution.

2.2 Microstructure and Composition of the Coatings

The surface and cross-section morphology and the chemical composition of the fabricated layered composite coatings were examined via a scanning electron microscope (SEM, model Auriga, Zeiss) equipped with energy-dispersive X-ray spectroscopy (EDX). The cross-sectional samples were prepared by Ar ion milling system (IM4000, HITACHI). X-ray diffraction (XRD) was performed using an X’pert Philips MPD instrument (equipped with Panalytical X’celerator detector, Germany) employing graphite monochromized Cu Kα radiation (Wavelength 1.54056 Å).

2.3 Electrochemical Measurements

Electrochemical behavior of the samples was investigated by using an electrochemical workstation “IM6eX” (Zahner-Elektrik GmbH and Co. KG, Kronach, Germany). Dulbecco’s modified Eagle’s medium (DMEM, Biochrome AG) was used as the electrolyte. The measurements were carried out in a conventional three-electrode cell assembly which consisted of the coated sample as the working electrode, a platinum plate as the auxiliary electrode and an Ag/AgCl electrode in 3-M KCl as the reference electrode. Long-term open-circuit potential (OCP) was monitored continuously up to 8 h in DMEM at room temperature. DMEM was refreshed every 8 h to mimic the physical buffering environment. To accelerate corrosoin, the samples were potentiodynamically polarized with sweep rate of 3 mV/s into the anodic direction.

3 Results and Discussion

3.1 Microstructure and Composition

Morphology and elemental composition of freshly prepared samples were studied via SEM coupled with EDX and representative results are shown in Figure 2. EDX analysis was carried out on the whole surface and at special locations to determine the composition of the layered composite coating. Clusters, as magnified in Figure 2A, precipitated randomly on the entire surface. These clusters were principally composed of metallic iron, such as at Spot 2 in Figure 2B. The iron signal indicates successful chemical conversion reaction, namely reduction of Fe3+ into Fe by the less noble Mg substrate (subsequent XRD results confirm this observation as discussed below). In the general survey 62.43 wt% iron was detected, with 25.73 wt% of O, 8.27 wt% of Mg, and 3.57 wt% of Cl. The small amount of chlorine is incorporated in the coating as a contamination from the reaction solution. The weight percentage of Mg at Spot 1 in Figure 2A, that is the rather flat area between the clusters, represents the highest value (12.34 wt%) compared with that of the whole area and that in Spot 2. In any case, the surface of the sample mostly consists of iron.


FIGURE 2. SEM images. (A) with EDX analysis (B) of a freshly prepared sample.

3.2 Crystal Structure of the Coatings

XRD measurements were conducted to characterize the crystal structure of the formed coatings. Figure 3 shows the XRD patterns of pure Mg, pure Fe and the treated sample. All peaks in the pattern of the hybrid laminate appear in the exactly corresponding position to those of pure Mg and pure Fe substrates. Besides, a weak peak at approx. 60°, 2θ are assigned to Mg(OH)2. XRD results therefore confirm that the layered composite coating is mainly composed of metallic Mg and Fe, and small amount of Mg(OH)2, which is in good agreement with the EDX results.


FIGURE 3. X-ray diffraction patterns of pure Mg, pure Fe and the freshly prepared composite laminate.

The proposed formation mechanism of the particular structure shown in Figure 2A is schematically illustrated in Figure 4. The chemical reactions occurring during the dipping process are schematically shown to explain the mechanism. As Mg is less noble than Fe, metallic Fe can be formed on Mg from the Fe3+ ion containing solution. Therefore, upon immersion of pure Mg samples in FeCl3 solution, the following chemical reactions take place:

3Mg+2Fe3+2Fe+3Mg2+Mg+2H2OMg(OH)2 +H2


FIGURE 4. Schematic illustration for the formation of the coating.

The sites of Fe3+ ion reduction and deposition as metallic Fe coincide with the sites of metallic Mg oxidation into Mg2+ and its dissolution. Simultaneously, with the hydrolysis of water, H2 is produced and OH released. Mg2+ can easily precipitate with OH as Mg(OH)2, hence depositing on the surface as well.

After 2 weeks storage in air, the surface morphology and chemical composition of the prepared samples were observed again by SEM coupled with EDX, as shown in Figure 5. Compared with the fresh sample, a characteristic “pompon-like” structure is formed at the sites between clusters, for instance near Spot 3 and framed areas in Figure 5A. The framed areas are shown in magnification to reveal the nano scale features on the surface, showing the presence of nano needles or nano wires branching out in all directions from the center of the pompon. EDX analysis revealed that the locations of the pompons are primarily enriched with Mg (35.89 wt%) and O (57.98 wt%), but contain only traces of Fe (0.1 wt%), such as in Spot 3. Conversely, much less Mg (4.61 wt%) and O (4.04 wt%) are detected in other areas, such as in Spot 5, where the random disorganized clusters are observed, while 84.43 wt% Fe is discovered in this spot. As regards the chemical composition in Spot 4, the total weight percentage of Mg and O takes up more than 99% throughout the coating. In addition, many fine cracks are observed, which is a typical observation for conversion coatings on magnesium (Cui et al., 2012). Storage in air results in strongly increasing amounts of O and Mg on the whole surface, namely, from 25.73 wt% and 8.27 wt% in the fresh sample (in Figure 2B) up to 49.79 wt% and 37.32 wt% after lying in air (Figure 5B). Since Mg is more active than Fe under atmospheric conditions, galvanic corrosion has occurred between these two metals. As a result, Mg has been oxidized into pompon-like MgO/Mg(OH)2 structures gradually forming on the whole surface, and metallic iron has remained unattacked. The process is schematically shown in Figure 6. Eventually, the resulting structure is a metallic Fe coating buried under a Mg(OH)2 layer, as shown by the cross-sectional element mapping in Figure 7.


FIGURE 5. SEM images. (A) of the surface and EDX analysis. (B) of a sample after 2 weeks exposure to air.


FIGURE 6. Schematic illustration to represent the oxidation process on investigated samples.


FIGURE 7. SEM images and EDX mapping of the cross-section of the hybrid laminate after 2 weeks exposure to air.

3.3 Cross-Section Morphology and Composition

For observing the morphology and element distribution from cross sections of the hybrid laminate after 2-week exposure to ambient air, SEM observations and EDX elemental mapping were carried out, as shown in Figure 7. The total thickness of the coating was determined to be approximately 19.4 µm. Elemental mapping of the cross-section revealed an uneven distribution of Mg, O, Fe, and Cl through the thickness of the coating. The top layer contains mainly Mg and O, this layer also shows cracks which are typical for MgO/Mg(OH)2 surface layers. In addition, the chlorine contamination is only present in the uppermost part of the surface layer. Interestingly, the Fe coating with a thickness of 1–2 µm is buried under the top MgO/Mg(OH)2 layer, as suggested in the schematic diagram in Figure 6. Additional MgO/Mg(OH)2 formation can be seen at sites of cracks through the top MgO/Mg(OH)2 and the Fe layers.

3.4 Corrosion Behavior

3.4.1 OCP Measurements

To investigate the corrosion behavior of the new layered metals, long-term open-circuit potential (OCP) measurements were carried out in DMEM for the coated sample, as well as for pure Fe and pure Mg. The OCP variation as a function of time for the different samples is shown in Figure 8. Clearly, pure Mg and pure Fe samples reach a rather stable value around −1.75 V after 2500 s and −0.7 V after 4000 s, respectively, whereas the OCP of the coated sample fluctuated markedly between −0.2 V and −1.2 V during the first 8 h immersion time. However, interestingly, the OCP always recovered back to near −0.2 V every time after a sharp drop down to values close to −1.2 V. These oscillations in the OCP indicate breakdown events in the coating (sharp drop of OCP), followed by “self-healing” of the coating, for instance by precipitation of Mg(OH)2 or other corrosion products blocking the sites of coating breakdown. Similar oscillations occurred again during the 27–35 h OCP observation, but the potential in this case recovers back to around -500mV and the fluctuation frequency is reduced compared with that during the first 0–8 h of immersion. After 46 h immersion in DMEM, OCP dropped down to around -1200mV and stayed stable in the following 4 h.


FIGURE 8. Open-circuit potential as a function of immersion time in DMEM.

The “self-healing” effect in OCP curves mainly embodies the “pulling up” of the dropped OCP values to a much higher OCP level. It can be speculated that the pulling-up and dropping-down processes shown in OCP curves are strongly linked to the initiating and hindering of the galvanic reactions which are non-continuously happening between the magnesium substrate and the iron coating in DMEM. In the first 8 h of OCP measurement, the upper level of OCP curves was maintained at around −0.2 V. This upper level of OCP values was even less negative than that of pure iron, which stayed at around −0.7 V after 8 h tests. The reason behind this unique phenomenon might be the existence of iron oxides and other depositions in DMEM precipitating on the surface of the magnesium substrate. Meanwhile, galvanic reactions would be initiated at the defect sites where the magnesium substrate was not fully coated by metallic iron coatings and the deposited products. At this stage, the OCP curve demonstrates a sharp drop-down to a lower level, which reflects the proceeding of galvanic reactions. The galvanic reaction products, mainly composed of Mg(OH)2 and other hydrated and carbonated (Mg,Ca)-phosphate (Wagener and Virtanen, 2016) formed near the micro-crack sites, would separate the connection between the magnesium substrate and the iron coating in the medium and impeding the galvanic reaction process. The prevailed “OFF” mode of the galvanic reaction induces the “self-healing” effect in OCP curves and the occasional “ON” mode of the galvanic reaction leads to the lower OCP level. The similar case can also be found in the curves of 27–35 h OCP, only the sharp potential dropping-down times and upper level of OCP decrease. The pH variation curve during the first 6 h of immersion and the potentiodynamic polarization curves of samples are displayed in the Supporting Information part.

In order to investigate changes in the morphology during the long-term OCP immersion experiment, samples were observed after 8 h (in Figures 9A,B) and 50 h (in Figures 9C,D) OCP monitoring in DMEM. As shown in Figure 9A, randomly distributed clusters are still displayed on the whole surface after 8 h OCP measurement. In the magnified images, many precipitates deposited on the surface can be observed. A typical magnesium conversion coating morphology with cracks is observed after 50 h OCP monitoring in Figure 9C. Protuberances, such as in Spot 11, could be seen on the separated pieces of the cracked coating in high magnification images in Figure 9C. The results in Figure 9B,D show the coating composition in overview for the whole surface and for three different sites. Since the depositions formed on the top surface, EDX data in Figures 9B,D shows a nearly 50% reduced iron signals as compared with a fresh sample (in Figure 2B). On the other hand, the O content increased significantly from 25.73 wt% in Figure 2B to about 50 wt% in Figures 9B,D. With longer immersion time in DMEM, the weight percentage of Mg increased from ca. 10 wt% at 8 h up to 30 wt% at 50 h. The growing amount of O and Mg coincides with the formation of the typical cracked coating, as observed in Figure 9C.


FIGURE 9. SEM images with EDX analysis of the hybrid layers after 8 h (A,B) and 50 h (C,D) OCP monitoring in DMEM.

Compounds containing Na, P, K, and Ca are incorporated in the surface layer and stem from the DMEM solution, which was employed as electrolyte for the OCP measurements. Overall, the levels of Na, P, K and Ca decrease from the values at 8 h in Figure 9B to the values at 50 h in Figure 9D. The decreasing trend might be due to the fact that the compounds containing such elements are buried by the subsequently formed Mg(OH)2 on top.

3.4.2 Micromorphology and Composition After Electrochemical Measurements

To demonstrate the coating behavior under a highly accelerated corrosion scenario, the coated samples were polarized up to +1 V (this condition does not correspond to a normal corrosion scenario of Mg alloys, but the experiment was carried out to indicate a worst-case behavior). Corrosion products after the electrochemical measurement were examined by SEM along with EDX to determine the morphology and chemical composition. As shown in Figure 10A, the coating is still present on the surface, exhibiting some large clusters of compact precipitates. The tiny cracks spread between or through the clusters on the rough surface. The chemical composition of the corrosion products was analyzed by EDX. The results in Figure 10 2) show the composition in overview for the whole surface and for three different sites. For the overview, strong signals for Fe (54.53 wt%) and O (34.17 wt%) are observed, with some Ca (5.17%) and minor amounts of Mg, Na, Cl, P and K (in total 5 wt%). A similar case for element distribution is found at Spot 13 and Spot 14 in Figure 10B. The Fe content in Spot 12 (73.69 wt%) is even higher than in the whole surface. The comparable presence of Fe and the significant decreased Mg content after the electrochemical measurement as compared with the average amount present in the fresh treated samples (see Figure 2B) may be attributed to the unstable MgO or Mg(OH)2 and other components in the top layer that have dissolved during the testing period in DMEM, exposing the underlying Fe compact coating, as shown in Figure 10A. Noteworthy is that the Fe coating has not been destroyed by polarization up to + 1 V in DMEM. The Fe coating will work as a barrier against the penetration of external corrosive ions onto the magnesium substrate. Furthermore, the weight percentage ratio of oxygen to iron in the fresh sample (Figure 2) and after electrochemical tests (Figure 10) increases approximately from 1:3 to 1:2, revealing partial oxidation of the metallic iron.


FIGURE 10. SEM image (A) and EDX result (B) of the surface morphology of hybrid laminate after electrochemical tests.

4 Conclusion and Outlook

A simple, single-step dip coating process was used to fabricate a layered composite coating composed of metallic Fe and Mg(OH)2 on a Mg substrate. A metallic Fe coating with a thickness of 1–2 µm is formed between the Mg substrate and a Mg(OH)2/MgO top layer. Under immersion conditions in DMEM, the open-circuit potential of the coated samples shows large oscillations, indicating local breakdown events followed by a self-healing behavior of the coated samples.

The simple coating process developed here; based on spontaneous electrochemical reactions, combined with the self-healing nature of the composite coating, represent a promising approach for tackling the challenge of controlling the corrosion rate of both Mg and Fe. Further research will focus on attempting to increase the thickness of the coatings and decrease the presence of cracks in the coatings, as well as on further elucidation of the degradation mechanisms and the origin of the self-healing property of the coatings.

Data Availability Statement

The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding author.

Author Contributions

YY, SV, and AB have substantial contributions to the conception and design of the work. YY collected, analyzed, and interpretation of data for the work. XC, GJ, and PS organized the structure of the manuscript. ZS and YL were responsible for the experiments of pH variation and potentiodynamic polarization during the revision period. All authors contributed to manuscript revision, read, and approved the submitted version.


YY would like to acknowledge China Postdoctoral Science Foundation (No. 3236310536), Heilongjiang Postdoctoral Science Foundation (No. 002100830603), and Fundamental Research Funds for the Central Universities (No. 3072021CF1005).

Conflict of Interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s Note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors, and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.


The authors would like to thank Lei Wang (Chair for Surface Science and Corrosion) and Martin Weiser (Chair for Surface Science and Corrosion) from University of Erlangen-Nuremberg, Germany, for XRD and cross-section experimental supports.

Supplementary Material

The Supplementary Material for this article can be found online at:


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Keywords: iron, magnesium, one-step fabrication, composite, “self-healing” effect

Citation: Yang Y, Shi Z, Cui X, Liu Y, Jin G, Virtanen S, Boccaccini AR and She P (2021) Preliminary Studies for One-Step Fabrication of Metallic Iron-Based Coatings on Magnesium as Temporary Protection in Biodegradable Medical Application. Front. Mater. 8:786650. doi: 10.3389/fmats.2021.786650

Received: 30 September 2021; Accepted: 15 November 2021;
Published: 08 December 2021.

Edited by:

Lechun Xie, Wuhan University of Technology, China

Reviewed by:

Lin Mao, University of Shanghai for Science and Technology, China
Jun Wang, Deakin University, Australia
Davod Seifzadeh, University of Mohaghegh Ardabili, Iran

Copyright © 2021 Yang, Shi, Cui, Liu, Jin, Virtanen, Boccaccini and She. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Xiufang Cui,; Peng She,