Impact Factor 4.298

The 1st most cited journal in Plant Sciences

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2018.00260

Seed-Specific Expression of AtLEC1 Increased Oil Content and Altered Fatty Acid Composition in Seeds of Peanut (Arachis hypogaea L.)

 Guiying Tang1,  Pingli Xu1, Wenhua Ma1, 2,  Fang Wang1, 3,  Zhanji Liu4, Shubo Wan1, 2* and  Lei Shan1, 2, 3*
  • 1Bio-tech Research Center, Shandong Academy of Agricultural Sciences, China
  • 2College of Life Science, Shandong University, China
  • 3College of Life Science, Shandong Normal University, China
  • 4Shandong Cotton Research Center, Shandong Academy of Agricultural Sciences, China

Abstract: Peanut (Arachis hypogaea L.) is one of the major oil crops and is the fifth largest source of plant oils in the world. Numerous genes participate in regulating the biosynthesis and accumulation of the storage lipids in seeds or other reservoir organs, among which several transcription factors, such as LEAFY COTYLEDON1 (AtLEC1), LEC2, and WRINKLED1 (WRI1), involved in embryo development also control the lipid reservoir in seeds. In this study, the AtLEC1 gene was transferred into the peanut genome and expressed in a seed-specific manner driven by the NapinA full-length promoter or its truncated 230-bp promoter. Four homozygous transgenic lines, two lines with the longer promoter and the other two with the truncated one, were selected for further analysis. The AtLEC1 mRNA level and the corresponding protein accumulation in different transgenic overexpression lines were altered, and the transgenic plants grew and developed normally without any detrimental effects on major agronomic traits. In the developing seeds of transgenic peanuts, the mRNA levels of a series of genes were upregulated. These genes are associated with fatty acid biosynthesis and lipid accumulation. The former set of genes included the homomeric ACCase A (AhACC II), the BC subunit of heteromeric ACCase (AhBC4), ketoacyl-ACP synthetase (AhKAS II), and stearoyl-ACP desaturase (AhSAD), while the latter ones were the diacylglycerol acyltransferases and oleosins (AhDGAT1, AhDGAT2, AhOle1, AhOle2, and AhOle3). The oil content and seed weight increased by 4.42%–15.89% and 11.1%–22.2%, respectively, and the levels of major fatty acid (FA) components including stearic acid, oleic acid, and linoleic acid changed significantly in all different lines.

Keywords: Transgenic peanut (Arachis hypogaea L.), Arabidopsis LEC1 (AtLEC1) overexpression, Seed-specific promoter, fatty acid synthesis, oil accumulation

Received: 04 Jan 2018; Accepted: 14 Feb 2018.

Edited by:

Chandrashekhar P. Joshi, Michigan Technological University, United States

Reviewed by:

Nookaraju Akula, Kaveri Seed Company Ltd., India
Anindya Ganguly, Department of Biology, Washington University in St. Louis, United States  

Copyright: © 2018 Tang, Xu, Ma, Wang, Liu, Wan and Shan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Mr. Shubo Wan, Shandong Academy of Agricultural Sciences, Bio-tech Research Center, No.202 Gongyebei Road, Licheng District, Jinan, 250100, Shandong Province, China, wansb@saas.ac.cn
Dr. Lei Shan, Shandong Academy of Agricultural Sciences, Bio-tech Research Center, No.202 Gongyebei Road, Licheng District, Jinan, 250100, Shandong Province, China, shlei1025@sina.com