DNA Mini-barcoding: A Derived Barcoding Method for Herbal Molecular Identification
- 1Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, China
In recent years, demand for natural herbal products (NHP) has increased; however, the quality of these products is difficult to confirm due to the lack of a comprehensive quality control system. Traditional methods are not effective for detecting processed ingredients. DNA barcoding is an established technique that has been used for >10 years. This technique uses short standard sequences (generally 200–600 bp) to identify species. While a complete DNA barcode is difficult to obtain from NHP due to DNA degradation, mini-barcoding is a complementary tool to identify species in NHP. DNA mini-barcoding uses smaller DNA segments for polymerase chain reaction amplification, and can be applied to rapidly identify species. The present review summarizes the development and application of DNA mini-barcodes over recent years, and discusses the limitations of this technique. This review also compares mini-barcoding and meta-barcoding, a technique using universal polymerase chain reaction primers to simultaneously amplify multiple DNA barcodes and identify many species in a single environmental sample. Additionally, other detection methods that can be combined with mini-barcodes, such as nucleotide signatures, high-resolution DNA melting analysis, and gold nanoparticles, are discussed. DNA mini-barcoding can fill the gaps left by other methods in the field of herbal molecular identification.
Keywords: DNA barcoding, biomarker, Natural herbal products, DNA mini-barcode, Molecular idenlification
Received: 31 Jul 2018;
Accepted: 12 Jul 2019.
Edited by:Roger Deal, Emory University, United States
Reviewed by:Panagiotis Madesis, Institute of Applied Biosciences (INAB), Greece
G. Ravikanth, Ashoka Trust for Research in Ecology and the Environment (ATREE), India
Copyright: © 2019 Gao, Wang, Wei, Liu and Han. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Dr. Jianping Han, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing, China, firstname.lastname@example.org