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ORIGINAL RESEARCH article

Front. Plant Sci.
Sec. Functional and Applied Plant Genomics
Volume 15 - 2024 | doi: 10.3389/fpls.2024.1395223
This article is part of the Research Topic Wheat Disease Resistance: Diagnosis, Germplasm Mining, and Molecular Breeding View all 14 articles

Fine Mapping of QYrsv.swust-1BL for Resistance to Stripe Rust in Durum Wheat Svevo

Provisionally accepted
Xinli Zhou Xinli Zhou 1Guoyun Jia Guoyun Jia 1Yuqi Luo Yuqi Luo 1Xin Li Xin Li 1Lin Cai Lin Cai 2*Xianming Chen Xianming Chen 3Zhensheng Kang Zhensheng Kang 4*
  • 1 School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, China
  • 2 Guizhou University, Guiyang, Guizhou Province, China
  • 3 US Department of Agriculture, Agricultural Research Service, Wheat Health, Genetics, and Quality Research Unit and Department of Plant Pathology, Washington State University, Pullman, WA, United States
  • 4 College of Plant Protection, Northwest A&F University, Yangling, China

The final, formatted version of the article will be published soon.

    Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a serious disease in wheat worldwide. There is a great need to develop cultivars with combinations of all stage resistance (ASR) and adult-plant resistance (APR) genes for sustainable control of the disease. QYrsv.swust-1BL in Italian durum wheat (Triticum turgidum ssp. durum) cultivar Svevo is effective against Pst races in China and Israel, and the gene has been previously mapped to the long arm of chromosome 1B. The gene flanked by SNP (single nucleotide polymorphism) markers IWB5732 and IWB4839 (0.75 cM). In the present study, we used high-density 660K SNP array genotyping and the phenotypes of 137 recombinant inbred lines (RILs) to fine map the QYrsv.swust-1BL locus within a 1.066 Mb region in durum wheat Svevo (RefSeq Rel. 1.0) on chromosome arm 1BL.The identified 1.066 Mb region was overlaps with a previously described map of Yr29/QYr.ucw-1BL, a stripe rust APR gene. Twenty five candidate genes for QYrsv.swut-1BL were identified through comparing polymorphic genes within the 1.066 Mb region in the resistant cultivar. SNP markers were selected and converted to Kompetitive allele-specific polymerase chain reaction (KASP) markers. Five KASP marker based on SNP was validated in a F2 and F2:3 breeding population, providing further compelling evidence for the significant effects of QYrsv.swut-1BL. These marker should be useful in marker-assisted selection for incorporating Yr29/QYrsv.swust-1BL into new durum and common wheat cultivars for resistance to stripe rust.

    Keywords: stripe rust, resistance gene, durum wheat, fine mapping, quantitative trait locus

    Received: 03 Mar 2024; Accepted: 06 May 2024.

    Copyright: © 2024 Zhou, Jia, Luo, Li, Cai, Chen and Kang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

    * Correspondence:
    Lin Cai, Guizhou University, Guiyang, 550025, Guizhou Province, China
    Zhensheng Kang, College of Plant Protection, Northwest A&F University, Yangling, 712100, China

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