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ORIGINAL RESEARCH article
Front. Plant Sci.
Sec. Crop and Product Physiology
Volume 15 - 2024 |
doi: 10.3389/fpls.2024.1412540
This article is part of the Research Topic Quality and Resistance Physiology and Regulation of Root and Tuber Crops View all articles
Transcriptome-and Genome-wide Systematic Identification of Expansin Gene Family and their Expression in Tuberous Root Development and Stress Responses in Sweetpotato (Ipomoea batatas)
Provisionally accepted
Jianling Zhang
1*
Tingting Dong
2
Mingku Zhu
2
Dan Du
3
Ranran Liu
1
Qianqian Yu
1
Yueying Sun
1
Zhihuan Zhang
4*- 1 Liaocheng University, Liaocheng, Shandong Province, China
- 2 Jiangsu Normal University, Xuzhou, Jiangsu Province, China
- 3 Southwest University, Chongqing, Chongqing Municipality, China
- 4 Qingdao Academy of Agricultural Sciences, Qingdao, Shandong Province, China
Expansins (EXPs) are essential components of the plant cell wall that function as relaxation factors to directly promote turgor-driven expansion of the cell wall, thereby controlling plant growth and development and diverse environmental stress responses. EXPs genes have been identified and characterized in numerous plant species, but not in sweetpotato. In the present study, a total of 59 EXP genes unevenly distributed across 14 of 15 chromosomes were identified in the sweetpotato genome, and segmental and tandem duplications were found to make dominant contribution to the diversity of functions of the IbEXP family. Phylogenetic analysis showed that IbEXP members could clustered into four subfamilies based on the EXPs from Arabidopsis and rice, and the regularity of protein motif, domain, and gene structures was in consistent with this subfamily classification. Collinearity analysis between IbEXP genes and related homologous sequences in nine plants provided further phylogenetic insights into the EXP gene family. Cis-element analysis further revealed the potential roles of IbEXP genes in sweetpotato development and stress responses. RNA-seq and qRT-PCR analysis of eight selected IbEXPs genes provided evidence of their specificity in different tissues, and showed that their transcripts were variously induced or suppressed under different hormone treatments (abscisic acid, salicylic acid, jasmonic acid, and 1-aminocyclopropane-1-carboxylic acid) and abiotic stresses (low and high temperature). These results provide a foundation for further comprehensive investigation of the functions of IbEXP genes, and indicate that several members of this family have potential applications as regulators to control plant development and enhance stress resistances of plants.
Keywords: Sweetpotato, Expansin gene family, Systematic identification, RNA-Seq, Tuberous root development, plant hormones, stress response
Received: 05 Apr 2024; Accepted: 14 May 2024.
Copyright: © 2024 Zhang, Dong, Zhu, Du, Liu, Yu, Sun and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence:
Jianling Zhang, Liaocheng University, Liaocheng, 252000, Shandong Province, China
Zhihuan Zhang, Qingdao Academy of Agricultural Sciences, Qingdao, 266000, Shandong Province, China
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