ORIGINAL RESEARCH article
Front. Chem.
Sec. Chemical Physics and Physical Chemistry
This article is part of the Research TopicAdvanced Functional Materials, Structures and Devices for Advancing Human Healthcare ApplicationsView all 8 articles
Sensitive Immunosensing of Melanoma Biomarker Based on Enhanced Electrochemiluminescence via Electronic Metal-Support Interactions
Provisionally accepted
- 1Shanxi Bethune Hospital, Taiyuan, China
- 2Zhejiang Sci-Tech University, Hangzhou, China
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Developing highly sensitive and convenient immunosensor for the detection of biomarker is important for enhancing the effectiveness of melanoma prevention and control measures. In this work, immunosensor was fabricated for sensitive detection of the melanoma biomarker S100B based on enhanced electrochemiluminescence (ECL) via electronic metal-support interactions. CoAl-layered double hydroxide (LDH) was selected as to modify the costless indium tin oxide (ITO) electrode due to its high surface area and tunable structure. To improve its conductivity and electron transfer capability, oxygen vacancies (Ov) were introduced on LDH through an alkaline etching process, resulting in the LDH-Ov structure. Platinum nanoparticles (Pt) were then in situ loaded onto the LDH-Ov surface (Pt@LDH-Ov/ITO). The electrocatalytic metal-support interaction (EMSI) between LDH-Ov and Pt nanoparticles played a critical role in improving the catalytic activity, leading to an enhanced ECL signal in the luminol-dissolved oxygen (DO) system. The immunorecognition interface was fabricated on Pt@LDH-Ov/ITO, enabling selective detection of S100B. The constructed immunosensor exhibited a linear detection range for S100B from 100 fg/mL to 100 ng/mL, with a limit of detection (LOD) of 65 fg/mL. The high performance and enhanced sensitivity of the immunosensor make it a promising tool for the early diagnosis, monitoring of recurrence, and personalized treatment of melanoma.
Keywords: immunosensor, Electrochemiluminescence, Layered double hydroxide, Electronic met-al-support interaction, Melanoma
Received: 20 Sep 2025; Accepted: 30 Nov 2025.
Copyright: © 2025 Pei, Jia, Xi and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Fengna Xi
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