ORIGINAL RESEARCH article

Front. Chem., 06 September 2022

Sec. Organic Chemistry

Volume 10 - 2022 | https://doi.org/10.3389/fchem.2022.969770

Design, synthesis and cytotoxic evaluation of novel betulonic acid-diazine derivatives as potential antitumor agents

  • 1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, China

  • 2. Chinese Academy of Inspection and Quarantine, Beijing, China

  • 3. School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China

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Abstract

With the purpose to improve antiproliferative activity, 26 new betulonic acid-diazine derivatives were designed and synthesized from betulinic acid. The anticancer activity of these semi-synthetic compounds was evaluated by MTT assay in both tumor cell lines and normal cell line. The results indicated that majority of new compounds exhibited improved antitumor activity compared with the parent compound betulonic acid. Compound BoA2C, in particular, had the most significant action with IC50 value of 3.39 μM against MCF-7 cells, while it showed lower cytotoxicity on MDCK cell line than cisplatin. Furthermore, we discovered that BoA2C strongly increased MCF-7 cell damage mostly by influencing arginine and fatty acid metabolism. In addition, the structure-activity relationships were briefly discussed. The results of this study suggested that the introduction of different diazines at C-28 could selectively inhibit different kinds of cancer cells and might be an effective way to synthesize potent anticancer lead compound from betulonic acid.

1 Introduction

Breast cancer (BC), as the most common cancer type among women, remains a major threat to the health and wellness accounting for nearly 30 percent of cancer deaths each year (Hendrick et al., 2021). Current treatments for BC are similar to those for other cancers, including surgery, chemotherapy, and radiation. These treatments are designed to eliminate tumors and improve the lives of patients. Chemotherapy, in particular, is still one of the most effective treatments in BC, interfering with tumor cell reproduction and proliferation using exclusive medications (Maughan et al., 2010). Betulinic acid (BA), which belongs to lupane-type pentacyclic triterpenoids, can arrest the cell cycles of BC at G1 phases to inhibit viability and migration of breast cancer cells (Damle et al., 2013; Hsu et al., 2015). It has been reported that oxidation of the hydroxyl group at position C-3 markedly enhanced the cytotoxic potency (Baratto et al., 2013; Zhang et al., 2014). Thus, betulonic acid (BoA), which had a ketone instead of a hydroxyl with β-configuration at the C-3 of BA, was used as a starting molecule in the synthetic transformations described in this research for the identification of novel anticancer drugs.

Nitrogen-containing heterocycles in the drug design are almost 60% of unique small-molecule drugs based on FDA database (Liu et al., 2019). In parallel, a large number of nitrogen-containing heterocyclic derivatives like pyrrole, pyrrolidine, pyridine, and pyrazine have been discovered and described for their antitumor properties (Taglieri et al., 2020). In this respect, the antineoplastic properties of BAM15, a furazano [3,4-b] pyrazine, was demonstrated in breast cancer through uncoupling oxidative phosphorylation from ATP production (Zunica et al., 2021). Furthermore (3R,4S)-3-(4-bromophenyl)-4-(4-fluorobenzoyl)-2-(2-oxo-2-phenylethyl)-3,4-dihydropyrrolo [1,2-a] pyrazin-2-ium bromide, synthesized based on pyrrolo [1,2-a] pyrazine, was suggested as a novel potential anticancer agent via caspase-3 activation and PARP cleavage (Seo et al., 2020). Previously, we had reported the preparation of a series of novel natural product derivatives with ligustrazine (2,3,5,6-tetramethylpyrazine, TMP) which was a major effective component of Ligusticum chuanxiong Hort and observed that these derivatives possessed potent selective cytotoxicity (Xu et al., 2015; Bi et al., 2016; Xu et al., 2017; Wang H. et al., 2018; Wang P. et al., 2018; Fang et al., 2018). Meanwhile, we synthesized hederagenin derivatives containing pyrazines with different methyl numbers and positions, and most of these derivatives showed much stronger cytotoxicity than the parent compound (Fang et al., 2018).

BoA has two major functional groups, 3-=O and 28-COOH, which are susceptible to functionalization by transformations such as amination, esterification, sulfonation, hydroxylation, and alkylation. And many of these derivatives (Figure 1) are known to possess various types of biological activities (Saxena et al., 2006; Dangroo et al., 2017; Eignerova et al., 2017; Gupta et al., 2017; Khlebnicova et al., 2017; Mzhelskaya et al., 2017; Khlebnicova et al., 2019; Popov et al., 2019; Sousa et al., 2019; Hoenke et al., 2020; Yang et al., 2020). A previous study demonstrated that BoA derivatives with fused azole and triazine displayed impressive cytotoxic activity against several cancer cell lines (Dinh Ngoc et al., 2014; Grishko et al., 2017). And BoA derivatives of nitrogen-containing heterocycles at the C-28 position exhibited excellent anticancer activities (Yang et al., 2015). There are also studies that boc-lysinated-betulonic acid (100 μM) had been shown 95.7% inhibition of LNCaP prostate cancer cells whereas little effect on normally proliferating fibroblast cells (Saxena et al., 2006). Besides, a series of C-28 derived 1,2,3-triazolyl derivatives of BoA possessed promising cytotoxicity activity (IC50 value in the range of 4–6 μM) (Dangroo et al., 2017). The preceding investigations piqued our curiosity in whether BoA with nitrogen-containing heterocycles at C-28 might be beneficial for anticancer activities. In this study, we successfully synthesized 26 new BoA derivatives by introducing pyrazines including TMP, pyrimidines and pyridazines at the C-28 (Figure 2), in order to improve its anticancer activity. The cytotoxicity of all these derivatives (BoAA-BoAE, BoAnA-BoAnGn (n = 2, 3, 4)) was evaluated against four human cancer cell lines (including HepG2, Bel-7402, HeLa, MCF-7) and normal cell line Figure 2 (MDCK). And the ability of the most potent active compound to trigger cell injury was preliminary analyzed in vitro.

FIGURE 1

FIGURE 1

Structures of representative BoA derivatives with antitumor activity. The IC50 values of these compounds are shown in parentheses.

FIGURE 2

FIGURE 2

Illustration of the design strategy for target compounds.

2 Materials and methods

2.1 General aspects

Chemical shifts (δ) are given in ppm and coupling constants (J) in Hz. Reagents were bought from commercial suppliers without any further purification. NMR spectra were recorded on a Bruker-500 or -400 spectrometer (Bruker, Dresden, Germany) with tetramethylsilane (TMS; TCI, Tokyo, Japan) as an internal standard; high-resolution mass spectra (HRMS) were acquired using a Thermo Scientific TMLTQ Orbitrap XL hybrid FTMS instrument (Thermo Technologies, New York, NY, United States); Melting points were measured at a rate of 5°C/min using an X-5 micro melting point apparatus (Beijing, China) and were not corrected.

2.1.1 Preparation of BoA

Betulin (9.0 g, 61.02 mmol) was fully dispersed in acetone (800 ml). Neutral aluminum oxide (54 g) and K2Cr2O7 (18 g, 60 mmol) were added to a solution of H2SO4 (30 ml) in H2O (120 ml), which was added in batches into the Betulin suspensions after cooling to room temperature. The mixture was stirred for 3 h at 25°C. After completion of the reaction (as monitored by TLC), the mixture was filtered, evaporated, diluted with water (200 ml), extracted with ethyl acetate (3 × 100 ml) in sequence. Then the organic layer was dried over anhydrous sodium sulfate, filtrated and evaporated. The crude product was dissolved in tert-Butyl methyl ether (TBME) (100 ml), which was dropped into the 5% NaOH aqueous solution (200 ml), a lot of white precipitate was produced. After filtering, washing with TBME (3 × 10 ml), the BoA sodium salt was obtained. The solid was put in 3% hydrochloric acid (100 ml), extracted with ethyl acetate (3 × 50 ml), dried over anhydrous sodium sulfate, filtrated and evaporated. The precipitate was recrystallized from methanol. White flake crystal, 82.22% yield. m. p. 250–252°C. 1H NMR (500 MHz, CDCl3) δ (ppm) 1.38 (m, 1H, H-1a), 1.89 (m, 1H, H-1b), 2.41 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 1.32 (m, 1H, H-5), 1.44 (m, 1H, H-6a), 1.47 (m, 1H, H-6b), 1.42 (m, 2H, H2-7), 1.38 (o, 1H, H-9), 1.30 (m, 1H, H-11a), 1.46 (o, 1H, H-11b), 1.05 (m, 1H, H-12a), 1.72 (m, 1H, H-12b), 2.21 (td, J = 12.5, 2.5 Hz, 1H, H-13), 1.22 (br dt, J = 13.5, 2.5 Hz, 1H, H-15a), 1.54 (m, 1H, H-15b), 1.42 (o, 1H, H-16a), 2.28 (dt, J = 13.0, 3.0 Hz, 1H, H-16b), 1.63 (t, J = 11.5 Hz, 1H, H-18), 3.01 (td, J = 10.5, 5.0 Hz, 1H, H-19), 1.43 (o, 1H, H-21a), 2.00 (m, 1H, H-21b), 1.48 (o, 1H, H-22a), 1.98 (m, 1H, H-22b), 1.07 (s, 3H, CH3-23), 1.01 (s, 3H, CH3-24), 0.92 (s, 3H, CH3-25), 0.97 (s, 3H, CH3-26), 0.99 (s, 3H, CH3-27), 4.61 (brs, 1H, H-29E), 4.74 (d, J = 0.8 Hz, 1H, H-29Z), 1.69 (s, 3H, CH3-30). 13C NMR (125 MHz, CDCl3) δ (ppm) 39.7 (C-1), 34.3 (C-2), 218.3 (C-3), 47.5 (C-4), 55.1 (C-5), 19.8 (C-6), 33.7 (C-7), 40.8 (C-8), 50.0 (C-9), 37.1 (C-10), 21.5 (C-11), 25.6 (C-12), 38.7 (C-13), 42.6 (C-14), 29.8 (C-15), 32.2 (C-16), 56.5 (C-17), 49.3 (C-18), 47.0 (C-19), 150.4 (C-20), 30.7 (C-21), 37.2 (C-22), 26.8 (C-23), 21.1 (C-24), 16.1 (C-25), 16.0 (C-26), 14.8 (C-27), 182.3 (C-28), 109.9 (C-29), 19.5 (C-30). The above data were identical with the literature data (Ayers et al., 2016). HRMS (ESI) m/z: [M + H]+ 455.3523, calcd. for C30H47O3 455.3525.

2.1.2 Preparation of BoAnBr/BoAnOH (n = 2, 3, 4)

BoA (1.2 g, 2.64 mmoL) was dissolved in 20 ml dry DMF, then 1, 2-dibromoethane/1, 3-dibromopropane/1, 4-dibromobutane (13.20 mmol) and K2CO3 (5.28 mmoL) was added successively, and the mixture was stirred for 4 h at 25°C. Then the reaction mixture was diluted with water and then extracted with ethyl acetate. The combined organic layer was dried over anhydrous sodium sulfate and evaporated the solvent under vacuum. The crude product was purified by flash chromatography (silica gel, petroleum ether: acetone = 50:1). Then BoA2Br, BoA3Br, BoA4Br was obtained respectively.

When 1, 2-dibromoethane/1, 3-dibromopropane/1, 4-dibromobutane (13.20 mmol) was replaced with 2-bromoethanol/3-bromo-1-propanol (3.43 mmol) and other conditions were not changed, BoA2OH, BoA3OH was obtained separately.

When BoA (1.2 g, 2.64 mmol), 1, 2-dibromoethane/1, 3-dibromopropane/1, 4-dibromobutane (13.20 mmol) and K2CO3 (5.28 mmol) were replaced with BoA (2.0 g, 4.40 mmol), 4-chloro-1-butanol (5.72 mmol) and K2CO3 (13.2 mmol) and other conditions were not changed, BoA4OH was obtained.

2.1.3 General procedure for the synthesis of BoAA-BoAB

BoA (200 mg, 0.44 mmol) was dissolved in 10 ml dry DMF, then chloromethyl pyrazine (about 0.44 mmol) and K2CO3 (1.32 mmol) was added in sequence, the mixture was stirred for 2 h at room temperature. Then the reaction mixture was poured into water (50 ml) and the crude product was extracted with ethyl acetate (20 ml). The organic layer was washed with brine (20 ml), dried over sodium sulfate, filtrated and evaporated. Purification was performed by flash chromatography (silica gel, dichloromethane: methanol = 40:1).

2.1.3.1Pyrazin-2-ylmethyl-3-oxolup-20(29)-en-28-oate (BoAA)

BoAA was obtained as white powder; Yield: 49.89%; m. p. 79–81°C. 1H NMR (500 MHz, CDCl3) δ 8.68 (s, 1H, H-3′), 8.56 (t, J = 2.0 Hz, 1H, H-5′), 8.54 (d, J = 2.0 Hz, 1H, H-6′), 5.26 (m, 2H, CH2-2′), 4.60 (brs, 1H, H-29E), 4.72 (d, J = 2.0 Hz, 1H, H-29Z), 3.00 (td, J = 11.0, 5.0 Hz, 1H, H-19), 2.39 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.32 (dt, J = 13.0, 3.0 Hz, 1H, H-16b), 2.21 (td, J = 12.5, 3.5 Hz, 1H, H-13), 1.68, 1.06, 1.01, 0.96, 0.90, 0.83 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 175.6, 152.1, 150.4, 144.3, 144.1, 144.0, 109.9, 64.5, 56.8, 55.1, 50.0, 49.5, 47.5, 46.9, 42.6, 40.7, 39.7, 38.4, 37.0 ✕ 2, 34.3, 33.7, 32.1, 30.6, 29.8, 26.7, 25.6, 21.5, 21.2, 19.7, 19.5, 16.1, 15.8, 14.7. HRMS (ESI) m/z: [M + H]+ 547.3905, calculated for C35H51N2O3 547.3900.

2.1.3.2 (3-Methylpyrazin-2-yl)-methyl-3-oxolup-20(29)-en-28-oate (BoAB)

BoAB was obtained as white powder; Yield: 61.62%; m. p. 80–82°C. 1H NMR (400 MHz, CDCl3) δ 8.42 (s, 1H, H-5′), 8.38 (s, 1H, H-6′), 2.63 (s, 3H, CH3-3′), 5.25 (m, 2H, CH2-2′), 4.59 (s, 1H, H-29E), 4.71 (s, 1H, H-29Z), 3.00 (m, 1H, H-19), 2.38 (m, 1H, H-2a), 2.46 (m, 1H, H-2b), 2.27 (m, 1H, H-16b), 2.22 (m, 1H, H-13), 1.67, 1.06, 1.01, 0.95, 0.90, 0.84 (s, each 3H, 6 × −CH3). 13C NMR (100 MHz, CDCl3) δ 218.2, 175.6, 153.0, 150.5, 149.8, 143.4, 141.7, 109.8, 64.4, 56.8, 55.1, 50.0, 49.6, 47.5, 46.9, 42.6, 40.7, 39.8, 38.4, 37.1, 37.0, 34.3, 33.8, 32.1, 30.6, 29.7, 26.8, 25.6, 21.5, 21.3, 21.2, 19.8, 19.5, 16.1, 15.9, 14.7. HRMS (ESI) m/z: [M + H]+ 561.4055, calculated for C36H53N2O3 561.4056.

2.1.4 General procedure for the synthesis of BoAC-BoAD

To a solution of dichlorosulfoxide (106 mg, 0.88 mmol) in dichloromethane (20 ml), methyl-2-pyrazinylmethanol (110 mg, 0.88 mmol) was added. The reaction mixture was allowed to stir for 2 h at room temperature. After completion of the reaction (as monitored by TLC), the solution was evaporated. Then BoA (200 mg, 0.44 mmoL), dry DMF (10 ml), K2CO3 (1.32 mmol) was added in sequence, and the mixture was stirred for 4 h at room temperature. The reaction mixture was diluted with water (50 ml) and then extracted with ethyl acetate (20 ml). the organic layer was washed with brine (20 ml), dried over sodium sulfate, filtrated and evaporated. Purification was performed by flash chromatography (silica gel, dichloromethane: methanol = 40:1).

2.1.4.1 (5-Methylpyrazin-2-yl)-methyl-3-oxolup-20(29)-en-28-oate (BoAC)

BoAC was obtained as white powder; Yield: 52.70%; m. p. 125–127°C. 1H NMR (500 MHz, CDCl3) δ 8.54 (s, 1H, H-3′), 8.43 (s, 1H, H-6′), 2.58 (s, 3H, CH3-5′), 5.22 (m, 2H, CH2-2′), 4.59 (s, 1H, H-29E), 4.72 (s, 1H, H-29Z), 3.00 (td, J = 11.0, 5.0 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.30 (m, 1H, H-16b), 2.18 (m, 1H, H-13), 1.67, 1.06, 1.01, 0.96, 0.90, 0.83 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 175.7, 153.3, 150.4, 148.7, 144.0, 142.8, 109.9, 64.4, 56.8, 55.1, 50.0, 49.5, 47.5, 46.9, 42.6, 40.7, 39.8, 38.4, 37.0 ✕ 2, 34.3, 33.7, 32.1, 30.6, 29.8, 26.7, 25.6, 21.52, 21.5 ✕ 2, 21.2, 19.7, 19.5, 16.1, 15.8, 14.7. HRMS (ESI) m/z: [M + H]+ 561.4059, calculated for C36H53N2O3 561.4056.

2.1.4.2 (6-Methylpyrazin-2-yl)-methyl-3-oxolup-20(29)-en-28-oate (BoAD)

BoAD was obtained as white powder; Yield: 40.54%; m. p. 119–121°C. 1H NMR (500 MHz, CDCl3) δ 8.46 (s, 1H, H-3′), 8.40 (s, 1H, H-5′), 2.56 (s, 3H, CH3-6′), 5.22 (m, 2H, CH2-2′), 4.59 (brs, 1H, H-29E), 4.72 (d, J = 1.5 Hz, 1H, H-29Z), 3.00 (td, J = 11.5, 5.0 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.32 (dt, J = 13.0, 3.0 Hz, 1H, H-16b), 2.21 (td, J = 11.5, 2.5 Hz, 1H, H-13), 1.67, 1.05, 1.00, 0.96, 0.89, 0.82 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 175.6, 153.4, 150.8, 150.4, 143.8, 140.5, 109.9, 64.5, 56.8, 55.1, 50.0, 49.5, 47.5, 46.9, 42.6, 40.7, 39.7, 38.4, 37.0 × 2, 34.3, 33.7, 32.1, 30.6, 29.8, 26.7, 25.6, 21.6, 21.5, 21.2, 19.7, 19.5, 16.1, 15.8, 14.7. HRMS (ESI) m/z: [M + H]+ 561.4056, calculated for C36H53N2O3 561.4056.

2.1.5 General procedure for the synthesis of BoAE, BoAnB-BoAnE (n = 2,3,4)

BoA/BoAnBr (n = 2, 3, 4) (about 0.27 mmol, prepared as detailed in Section 4.1.2) was dissolved in 10 ml dry DMF, then chloroligustrazine/methyl-2-pyrazinic acid (about 0.27 mmol), K2CO3 (about 0.8 mmol) was added respectively, and the mixture was stirred for 4 h at 85°C. The reaction mixture was poured into water (50 ml) and then extracted with ethyl acetate (20 ml). The combined organic extracts were washed with brine (20 ml), dried over sodium sulfate, filtrated and evaporated. Then the crude product was purified by flash chromatography (silica gel, dichloromethane: methanol = 40:1).

2.1.5.1 (3,5,6-Trimethylpyrazin-2-yl)-methyl-3-oxolup-20(29)-en-28-oate (BoAE)

BoAE was obtained as white powder; Yield: 82.62%; m. p. 177–179°C. 1H NMR (500 MHz, CDCl3) δ 2.54 (s, 3H, CH3-3′), 2.51 (s, 3H, CH3-5′), 2.49 (s, 3H, CH3-6′), 5.18 (m, 2H, CH2-2′), 4.58 (brs, 1H, H-29E), 4.71 (d, J = 2.0 Hz, 1H, H-29Z), 2.99 (td, J = 11.5, 5.0 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.46 (m, 1H, H-2b), 2.27 (m, 1H, H-16b), 2.22 (m, 1H, H-13), 1.66, 1.05, 1.01, 0.94, 0.90, 0.82 (s, each 3H, 6 × -CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 175.6, 151.0, 150.6, 149.1, 148.8, 145.5, 109.8, 64.5, 56.8, 55.1, 50.0, 49.5, 47.5, 46.9, 42.6, 40.7, 39.7, 38.3, 37.0, 34.3, 33.7, 32.1, 30.6, 29.7, 26.7, 25.6, 21.8, 21.5 × 2, 21.2, 20.6, 19.7, 19.5, 16.1, 15.8, 14.7. HRMS (ESI) m/z: [M + H]+ 589.4370, calculated for C38H57N2O3 589.4369.

2.1.5.2 (3-Methylpyrazin-2-acyloxy)-ethyl-3-oxolup-20(29)-en-28-oate (BoA2B)

BoA2B was obtained as white powder; Yield: 74.42%; m. p. 60–62°C. 1H NMR (500 MHz, CDCl3) δ 8.62 (d, J = 2.0 Hz, 1H, H-5″), 8.52 (brs, 1H, H-6″), 2.85 (s, 3H, CH3-3″), 4.67, 4.62 (m, each 1H, CH2-1′), 4.47 (m, 2H, CH2-2′), 4.57 (s, 1H, H-29E), 4.68 (s, 1H, H-29Z), 2.95 (m, 1H, H-19), 2.38 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.24 (m, 1H, H-16b), 2.18 (td, J = 12.5, 3.5 Hz, 1H, H-13), 1.65, 1.05, 1.01, 0.93, 0.87, 0.84 (s, each 3H, 6 × -CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 175.9, 165.1, 155.5, 150.4, 146.2, 142.9, 141.6, 109.8, 63.8, 61.5, 56.7, 55.0, 49.9, 49.4, 47.5, 47.0, 42.5, 40.7, 39.7, 38.4, 37.0 × 2, 34.3, 33.6, 32.1, 30.6, 29.7, 26.7, 25.6, 23.4, 21.4, 21.2, 19.7, 19.5, 16.1, 15.8, 14.7. HRMS (ESI) m/z: [M + H]+ 619.4117, calculated for C38H55N2O5 619.4111.

2.1.5.3 (5-Methylpyrazin-2-acyloxy)-ethyl-3-oxolup-20(29)-en-28-oate (BoA2C)

BoA2C was obtained as white powder; Yield: 63.53%; m. p. 139–141°C. 1H NMR (500 MHz, CDCl3) δ 9.18 (s, 1H, H-3″), 8.59 (s, 1H, H-6″), 2.67 (s, 3H, CH3-5″), 4.72, 4.63 (m, each 1H, CH2-1′), 4.50, 4.43 (m, each 1H, CH2-2′), 4.57 (s, 1H, H-29E), 4.68 (d, J = 2.0 Hz, 1H, H-29Z), 2.96 (td, J = 11.0, 5.0 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.46 (m, 1H, H-2b), 2.24 (m, 1H, H-16b), 2.17 (td, J = 12.0, 3.0 Hz, 1H, H-13), 1.65, 1.05, 1.00, 0.93, 0.86, 0.84 (s, each 3H, 6 × -CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 175.9, 164.0, 158.2, 150.4, 145.5, 144.6, 140.4, 109.9, 63.7, 61.5, 56.7, 55.0, 49.9, 49.4, 47.4, 47.0, 42.5, 40.6, 39.7, 38.5, 37.0 × 2, 34.3, 33.6, 32.1, 30.6, 29.7, 26.7, 25.6, 22.2, 21.4, 21.2, 19.7, 19.5, 16.0, 15.8, 14.7. HRMS (ESI) m/z: [M + H]+ 619.4115, calculated for C38H55N2O5 619.4111.

2.1.5.4 (6-Methylpyrazin-2-acyloxy)-ethyl-3-oxolup-20(29)-en-28-oate (BoA2D)

BoA2D was obtained as white powder; Yield: 68.97%; m. p. 139–141°C. 1H NMR (500 MHz, CDCl3) δ 9.10 (s, 1H, H-3″), 8.64 (s, 1H, H-5″), 2.68 (s, 3H, CH3-6″), 4.72, 4.63 (m, each 1H, CH2-1′), 4.47 (m, 2H, CH2-2′), 4.57 (brs, 1H, H-29E), 4.68 (brs, 1H, H-29Z), 2.96 (td, J = 11.0, 4.5 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.25 (m, 1H, H-16b), 2.17 (td, J = 11.5, 3.5 Hz, 1H, H-13), 1.65, 1.05, 1.00, 0.93, 0.86, 0.85 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 175.9, 164.0, 154.5, 150.4, 148.0, 143.3, 142.2, 109.9, 63.8, 61.5, 56.7, 55.0, 49.9, 49.4, 47.4, 47.0, 42.5, 40.7, 39.7, 38.5, 37.1, 37.0, 34.3, 33.6, 32.1, 30.6, 29.7, 26.7, 25.6, 21.8, 21.4, 21.2, 19.7, 19.4, 16.0, 15.8, 14.7. HRMS (ESI) m/z: [M + H]+ 619.4112, calculated for C38H55N2O5 619.4111.

2.1.5.5 (3,5,6-Trimethylpyrazin-2-acyloxy)-ethyl-3-oxolup-20(29)-en-28-oate (BoA2E)

BoA2E was obtained as white powder; Yield: 70.03%; m. p. 74–76°C. 1H NMR (500 MHz, CDCl3) δ 2.75 (s, 3H, CH3-3″), 2.56 (s, 3H, CH3-5″), 2.55 (s, 3H, CH3-6″), 4.65, 4.60 (m, each 1H, CH2-1′), 4.48, 4.42 (m, each 1H, CH2-2′), 4.57 (brs, 1H, H-29E), 4.67 (d, J = 1.5 Hz, 1H, H-29Z), 2.96 (td, J = 11.0, 5.0 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.46 (m, 1H, H-2b), 2.25 (m, 1H, H-16b), 2.17 (td, J = 12.5, 3.5 Hz, 1H, H-13), 1.65, 1.05, 1.00, 0.93, 0.86, 0.82 (s, each 3H, 6 × -CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 175.9, 165.8, 154.8, 151.5, 150.5, 149.5, 139.3, 109.8, 63.5, 61.6, 56.7, 55.0, 49.9, 49.4, 47.4, 47.0, 42.5, 40.6, 39.7, 38.4, 37.0 × 2, 34.3, 33.6, 32.1, 30.6, 29.7, 26.7, 25.6, 22.8, 22.4, 21.7, 21.4, 21.2, 19.7, 19.5, 16.0, 15.7, 14.7. HRMS (ESI) m/z: [M + H]+ 647.4428, calculated for C40H59N2O5 647.4424.

2.1.5.6 (3-Methylpyrazin-2-acyloxy)-propyl-3-oxolup-20(29)-en-28-oate (BoA3B)

BoA3B was obtained as white oil; Yield: 60.64%; m. p. 54–55°C. 1H NMR (500 MHz, CDCl3) δ 8.62 (d, J = 2.5 Hz, 1H, H-5″), 8.53 (d, J = 2.0 Hz, 1H, H-6″), 2.85 (s, 3H, CH3-3″), 4.24 (m, 2H, CH2-1′), 2.20 (m, 2H, CH2-2′), 4.52 (t, J = 6.5 Hz, 2H, CH2-3′), 4.60 (brs, 1H, H-29E), 4.73 (d, J = 1.5 Hz, 1H, H-29Z), 2.99 (td, J = 11.5, 5.0 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.48 (m, 1H, H-2b), 2.26 (m, 1H, H-16b), 2.17 (m, 1H, H-13), 1.68, 1.06, 1.01, 0.96, 0.91, 0.90 (s, each 3H, 6 × -CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 176.1, 165.4, 155.4, 150.5, 146.1, 143.1, 141.6, 109.9, 63.0, 60.5, 56.7, 55.1, 50.0, 49.5, 47.5, 47.1, 42.6, 40.8, 39.8, 38.5, 37.1, 37.0, 34.3, 33.7, 32.2, 30.7, 29.8, 28.3, 26.8, 25.7, 23.4, 21.6, 21.2, 19.8, 19.5, 16.1, 15.9, 14.8. HRMS (ESI) m/z: [M + H]+ 633.4268, calculated for C39H57N2O5 633.4267.

2.1.5.7 (5-Methylpyrazin-2-acyloxy)-propyl-3-oxolup-20(29)-en-28-oate (BoA3C)

BoA3C was obtained as white powder; Yield: 67.92%; m. p. 57–58°C. 1H NMR (400 MHz, CDCl3) δ 9.18 (s, 1H, H-3″), 8.59 (s, 1H, H-6″), 2.67 (s, 3H, CH3-5″), 4.24 (m, 2H, CH2-1′), 2.20 (o, 2H, CH2-2′), 4.53 (t, J = 7.5 Hz, 2H, CH2-3′), 4.59 (s, 1H, H-29E), 4.72 (s, 1H, H-29Z), 2.99 (m, 1H, H-19), 2.39 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.24 (m, 1H, H-16b), 2.17 (m, 1H, H-13), 1.67, 1.05, 1.00, 0.96, 0.88 × 2 (s, each 3H, 6 × -CH3). 13C NMR (100 MHz, CDCl3) δ 218.2, 176.0, 164.2, 158.0, 150.5, 145.5, 144.5, 140.6, 109.9, 62.9, 60.4, 56.7, 55.1, 50.0, 49.5, 47.4, 47.1, 42.6, 40.7, 39.7, 38.5, 37.1, 37.0, 34.3, 33.7, 32.2, 30.7, 29.8, 28.2, 26.8, 25.6, 22.1, 21.5, 21.1, 19.8, 19.5, 16.1, 15.9, 14.7. HRMS (ESI) m/z: [M + H]+ 633.4269, calculated for C39H57N2O5 633.4267.

2.1.5.8 (6-Methylpyrazin-2-acyloxy)-propyl-3-oxolup-20(29)-en-28-oate (BoA3D)

BoA3D was obtained as white powder; Yield: 76.41%; m. p. 59–60°C. 1H NMR (500 MHz, CDCl3) δ 9.10 (s, 1H, H-3″), 8.64 (s, 1H, H-5″), 2.68 (s, 3H, CH3-6″), 4.24 (m, 2H, CH2-1′), 2.20 (o, 2H, CH2-2′), 4.54 (t, J = 6.5 Hz, 2H, CH2-3′), 4.60 (s, 1H, H-29E), 4.73 (s, 1H, H-29Z), 2.99 (td, J = 11.0, 4.5 Hz, 1H, H-19), 2.39 (m, 1H, H-2a), 2.48 (m, 1H, H-2b), 2.24 (m, 1H, H-16b), 2.17 (m, 1H, H-13), 1.67, 1.06, 1.00, 0.96, 0.89 × 2 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 176.1, 164.2, 154.4, 150.5, 147.9, 143.3, 142.4, 109.9, 63.0, 60.4, 56.7, 55.0, 50.0, 49.4, 47.5, 47.1, 42.6, 40.7, 39.7, 38.5, 37.1, 37.0, 34.3, 33.7, 32.2, 30.7, 29.7, 28.2, 26.7, 25.6, 21.9, 21.5, 21.2, 19.7, 19.5, 16.1, 15.9, 14.7. HRMS (ESI) m/z: [M + H]+ 633.4269, calculated for C39H57N2O5 633.4267.

2.1.5.9 (3,5,6-Trimethylpyrazin-2-acyloxy)-propyl-3-oxolup-20(29)-en-28-oate (BoA3E)

BoA3E was obtained as white powder; Yield: 75.49%; m. p. 57–59°C. 1H NMR (500 MHz, CDCl3) δ 2.74 (s, 3H, CH3-3″), 2.56 (s, 6H, CH3-5″, 6″), 4.23 (m, 2H, CH2-1′), 2.19 (o, 2H, CH2-2′), 4.48 (t, J = 6.5 Hz, 2H, CH2-3′), 4.59 (brs, 1H, H-29E), 4.72 (d, J = 2.0 Hz, 1H, H-29Z), 2.99 (td, J = 11.0, 5.0 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.24 (m, 1H, H-16b), 2.15 (m, 1H, H-13), 1.67, 1.05, 1.00, 0.95, 0.88 × 2 (s, each 3H, 6 × -CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 176.1, 166.0, 154.7, 151.3, 150.5, 149.5, 139.5, 109.8, 62.6, 60.6, 56.7, 55.0, 50.0, 49.4, 47.5, 47.0, 42.6, 40.7, 39.7, 38.4, 37.1, 37.0, 34.3, 33.7, 32.2, 30.7, 29.7, 28.2, 26.7, 25.6, 22.8, 22.3, 21.8, 21.5, 21.2, 19.7, 19.5, 16.1, 15.9, 14.7. HRMS (ESI) m/z: [M + H]+ 661.4582, calculated for C41H61N2O5 661.4580.

2.1.5.10 (3-Methylpyrazin-2-acyloxy)-butyl-3-oxolup-20(29)-en-28-oate (BoA4B)

BoA4B was obtained as colorless oil; Yield: 79.29%; m. p. 54–55°C. 1H NMR (500 MHz, CDCl3) δ 8.61 (d, J = 2.5 Hz, 1H, H-5″), 8.52 (d, J = 2.5 Hz, 1H, H-6″), 2.85 (s, 3H, CH3-3″), 4.14 (m, 2H, CH2-1′), 1.59 (m, 2H, CH2-2′), 1.80 (o, 2H, CH2-3′), 4.46 (t, J = 6.5 Hz, 2H, CH2-4′), 4.59 (brs, 1H, H-29E), 4.72 (d, J = 2.0 Hz, 1H, H-29Z), 3.00 (td, J = 11.0, 5.0 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.48 (m, 1H, H-2b), 2.25 (m, 1H, H-16b), 2.21 (m, 1H, H-13), 1.67, 1.06, 1.01, 0.96, 0.92, 0.89 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.4, 176.2, 165.4, 155.4, 150.6, 146.1, 143.2, 141.6, 109.8, 65.7, 63.4, 56.6, 55.1, 50.0, 49.4, 47.5, 47.1, 42.6, 40.8, 39.7, 38.4, 37.1, 37.0, 34.3, 33.7, 32.2, 30.7, 29.8, 26.7, 25.6 × 3, 23.4, 21.5, 21.2, 19.7, 19.5, 16.1, 15.9, 14.7. HRMS (ESI) m/z: [M + H]+ 647.4428, calculated for C40H59N2O5 647.4424.

2.1.5.11 (5-Methylpyrazin-2-acyloxy)-butyl-3-oxolup-20(29)-en-28-oate (BoA4C)

BoA4C was obtained as colorless oil; Yield: 76.57%; 1H NMR (500 MHz, CDCl3) δ 9.17 (d, J = 1.0 Hz, 1H, H-3″), 8.58 (s, 1H, H-6″), 2.66 (s, 3H, CH3-5″), 4.14 (m, 2H, CH2-1′), 1.70 (m, 2H, CH2-2′), 1.80 (m, 2H, CH2-3′), 4.47 (t, J = 6.5 Hz, 2H, CH2-4′), 4.58 (brs, 1H, H-29E), 4.71 (d, J = 2.0 Hz, 1H, H-29Z), 2.99 (td, 1H, J = 11.0, 4.5 Hz, H-19), 2.38 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.25 (m, 1H, H-16b), 2.21 (m, 1H, H-13), 1.67, 1.05, 1.00, 0.95, 0.92, 0.88 (s, each 3H, 6 × -CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 176.1, 164.3, 158.0, 150.6, 145.5, 144.5, 140.7, 109.8, 65.7, 63.4, 56.6, 55.0, 50.0, 49.4, 47.5, 47.1, 42.6, 40.7, 39.7, 38.4, 37.1, 37.0, 34.3, 33.7, 32.2, 30.7, 29.7, 26.7, 25.7, 25.6, 25.5, 22.1, 21.5, 21.2, 19.7, 19.5, 16.1, 15.9, 14.7. HRMS (ESI) m/z: [M + H]+ 647.4430, calculated for C40H59N2O5 647.4424.

2.1.5.12 (6-Methylpyrazin-2-acyloxy)-butyl-3-oxolup-20(29)-en-28-oate (BoA4D)

BoA4D was obtained as colorless oil; Yield: 79.00%; 1H NMR (500 MHz, CDCl3) δ 9.09 (s, 1H, H-3″), 8.63 (s, 1H, H-5″), 2.68 (s, 3H, CH3-6″), 4.14 (m, 2H, CH2-1′), 1.70 (m, 2H, CH2-2′), 1.80 (m, 2H, CH2-3′), 4.48 (t, J = 6.5 Hz, 2H, CH2-4′), 4.59 (brs, 1H, H-29E), 4.72 (d, J = 2.0 Hz, 1H, H-29Z), 2.99 (td, 1H, J = 11.0, 4.5 Hz, H-19), 2.38 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.25 (m, 1H, H-16b), 2.21 (m, 1H, H-13), 1.67, 1.05, 1.00, 0.96, 0.92, 0.88 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.4, 176.1, 164.3, 154.3, 150.6, 147.9, 143.2, 142.5, 109.8, 65.8, 63.4, 56.6, 55.0, 50.0, 49.4, 47.4, 47.1, 42.6, 40.7, 39.7, 38.4, 37.1, 37.0, 34.3, 33.7, 32.2, 30.7, 29.7, 26.7, 25.6 × 2, 25.5, 21.9, 21.5, 21.1, 19.7, 19.5, 16.1, 15.9, 14.7. HRMS (ESI) m/z: [M + H]+ 647.4437, calculated for C40H59N2O5 647.4424.

2.1.5.13 (3,5,6-Trimethylpyrazin-2-acyloxy)-butyl-3-oxolup-20(29)-en-28-oate (BoA4E)

BoA4E was obtained as white powder; Yield: 73.39%; m. p. 124–126°C. 1H NMR (500 MHz, CDCl3) δ 2.73 (s, 3H, CH3-3″), 2.55 (s, 6H, CH3-5″, 6″), 4.13 (m, 2H, CH2-1′), 1.71 (m, 2H, CH2-2′), 1.78 (m, 2H, CH2-3′), 4.42 (t, J = 6.5 Hz, 2H, CH2-4′), 4.58 (brs, 1H, H-29E), 4.71 (d, J = 1.5 Hz, 1H, H-29Z), 2.98 (td, 1H, J = 11.0, 4.5 Hz, H-19), 2.37 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.24 (m, 1H, H-16b), 2.20 (m, 1H, H-13), 1.66, 1.04, 0.99, 0.95, 0.90, 0.88 (s, each 3H, 6 × -CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 176.1, 166.0, 154.5, 151.1, 150.5, 149.5, 139.7, 109.8, 65.4, 63.5, 56.6, 55.0, 50.0, 49.4, 47.4, 47.0, 42.5, 40.7, 39.7, 38.4, 37.1, 37.0, 34.3, 33.7, 32.2, 30.7, 29.7, 26.7, 25.6 × 3, 22.7, 22.3, 21.8, 21.5, 21.1, 19.7, 19.5, 16.1, 15.9, 14.7. HRMS (ESI) m/z: [M + H]+ 675.4734, calculated for C42H63N2O5 675.4737.

2.1.6 General procedure for the synthesis of BoAnA, BoAnF-BoAnG (n = 2,3,4)

BoAnOH (n = 2, 3, 4) (about 0.3 mmol, prepared as detailed in Section 4.1.2) was dissolved in 10 ml dichloromethane, then 2-pyrazinic acid/4-pyrimidinic acid/4-pyridazinic acid (about 0.3 mmol), EDCI (about 0.3 mmol), DMAP (0.04 mmol) was added in sequence, and the mixture was stirred for 4 h at room temperature. The reaction mixture was diluted with water (50 ml), then the solution was extracted with dichloromethane (20 ml), washed with brine (20 ml), dried over anhydrous sodium sulfate, filtrated and evaporated. Then the crude product was purified by flash chromatography (silica gel, dichloromethane: methanol = 40:1).

2.1.6.1 (Pyrazin-2-acyloxy)-ethyl-3-oxolup-20(29)-en-28-oate (BoA2A)

BoA2A was obtained as white powder; Yield: 86.86%; m. p. 85–87°C. 1H NMR (500 MHz, CDCl3) δ 9.31 (d, J = 1.0 Hz, 1H, H-3″), 8.78 (d, J = 2.0 Hz, 1H, H-5″), 8.74 (t, J = 2.0 Hz, 1H, H-6″), 4.72, 4.66 (m, each 1H, CH2-1′), 4.48 (m, 2H, CH2-2′), 4.57 (s, 1H, H-29E), 4.68 (s, 1H, H-29Z), 2.96 (td, J = 11.0, 5.0 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.45 (m, 1H, H-2b), 2.25 (m, 1H, H-16b), 2.17 (td, J = 11.5, 3.5 Hz, 1H, H-13), 1.65, 1.05, 1.00, 0.93, 0.87, 0.86 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 175.9, 163.7, 150.4, 148.0, 146.4, 144.7, 143.2, 109.9, 64.0, 61.5, 56.7, 55.0, 49.9, 49.4, 47.5, 47.0, 42.5, 40.7, 39.7, 38.5, 37.1, 37.0, 34.3, 33.6, 32.1, 30.6, 29.7, 26.7, 25.6, 21.4, 21.2, 19.7, 19.5, 16.1, 15.8, 14.7. HRMS (ESI) m/z: [M + H]+ 605.3948, calculated for C37H53N2O5 605.3954.

2.1.6.2 (Pyrimidin-2-acyloxy)-ethyl-3-oxolup-20(29)-en-28-oate (BoA2F)

BoA2F was obtained as white powder; Yield: 79.00%; m. p. 72–74°C. 1H NMR (500 MHz, CDCl3) δ 9.41 (s, 1H, H-2″), 9.00 (d, J = 4.5 Hz, 1H, H-6″), 8.00 (d, J = 4.0 Hz, 1H, H-5″), 4.70, 4.63 (m, each 1H, CH2-1′), 4.47 (m, 2H, CH2-2′), 4.57 (s, 1H, H-29E), 4.67 (s, 1H, H-29Z), 2.94 (td, J = 11.0, 3.5 Hz, 1H, H-19), 2.37 (m, 1H, H-2a), 2.46 (m, 1H, H-2b), 2.23 (m, 1H, H-16b), 2.17 (td, J = 12.5, 2.5 Hz, 1H, H-13), 1.64, 1.04, 1.00, 0.93, 0.87, 0.86 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.2, 175.9, 163.8, 159.5, 159.3, 154.5, 150.4, 121.1, 109.9, 64.3, 61.3, 56.7, 55.1, 49.9, 49.4, 47.4, 47.0, 42.5, 40.7, 39.7, 38.5, 37.0 × 2, 34.2, 33.6, 32.1, 30.6, 29.7, 26.7, 25.6, 21.4, 21.1, 19.7, 19.4, 16.0, 15.8, 14.7. HRMS (ESI) m/z: [M + H]+ 605.3952, calculated for C37H53N2O5 605.3954.

2.1.6.3 (Pyridazin-2-acyloxy)-ethyl-3-oxolup-20(29)-en-28-oate (BoA2G)

BoA2G was obtained as white powder; Yield: 72.00%; m. p. 130–132°C. 1H NMR (500 MHz, CDCl3) δ 9.67 (brs, 1H, H-3″), 9.45 (d, J = 5.0 Hz, 1H, H-6″), 7.99 (dd, J = 5.0, 2.0 Hz, 1H, H-5″), 4.67, 4.61 (m, each 1H, CH2-1′), 4.46 (m, 2H, CH2-2′), 4.59 (s, 1H, H-29E), 4.69 (s, 1H, H-29Z), 2.96 (td, J = 11.5, 4.5 Hz, 1H, H-19), 2.37 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.23 (dt, J = 12.5, 3.0 Hz, 1H, H-16b), 2.17 (td, J = 11.5, 3.5 Hz, 1H, H-13), 1.65, 1.05, 1.00, 0.94, 0.87, 0.86 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.0, 175.9, 163.6, 152.2, 150.2, 149.7, 127.6, 125.6, 110.0, 64.4, 61.3, 56.8, 55.2, 50.0, 49.5, 47.5, 47.1, 42.6, 40.8, 39.8, 38.6, 37.1 × 2, 34.3, 33.8, 32.2, 30.7, 29.8, 26.8, 25.7, 21.5, 21.2, 19.7, 19.5, 16.0 × 2, 14.8. HRMS (ESI) m/z: [M + H]+ 605.3956, calculated for C37H53N2O5 605.3954.

2.1.6.4 (Pyrazin-2-acyloxy)-propyl-3-oxolup-20(29)-en-28-oate (BoA3A)

BoA3A was obtained as light yellow powder; Yield: 77.30%; m. p. 74–76°C. 1H NMR (500 MHz, CDCl3) δ 9.31 (s, 1H, H-3″), 8.78 (d, J = 2.5 Hz, 1H, H-5″), 8.74 (brs, 1H, H-6″), 4.25 (m, 2H, CH2-1′), 2.20 (o, 2H, CH2-2′), 4.54 (t, J = 6.5 Hz, 2H, CH2-3′), 4.59 (s, 1H, H-29E), 4.72 (s, 1H, H-29Z), 2.98 (m, 1H, H-19), 2.38 (m, 1H, H-2a), 2.45 (m, 1H, H-2b), 2.25 (m, 1H, H-16b), 2.19 (m, 1H, H-13), 1.67, 1.05, 1.00, 0.95, 0.88✕2 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.3, 176.0, 163.9, 150.5, 147.9, 146.4, 144.6, 143.4, 109.9, 63.2, 60.4, 56.7, 55.0, 50.0, 49.4, 47.4, 47.0, 42.6, 40.7, 39.7, 38.4, 37.1, 37.0, 34.3, 33.7, 32.2, 30.7, 29.7, 28.2, 26.7, 25.6, 21.5, 21.2, 19.7, 19.5, 16.1, 15.9, 14.7. HRMS (ESI) m/z: [M + H]+ 619.4115, calculated for C38H55N2O5 619.4111.

2.1.6.5 (Pyrimidin-2-acyloxy)-propyl-3-oxolup-20(29)-en-28-oate (BoA3F)

BoA3F was obtained as white powder; Yield: 48.06%; m. p. 50–52°C. 1H NMR (500 MHz, CDCl3) δ 9.42 (d, J = 1.5 Hz, 1H, H-2″), 9.00 (d, J = 5.0 Hz, 1H, H-6″), 8.02 (dd, J = 5.0, 1.0 Hz, 1H, H-5″), 4.25 (m, 2H, CH2-1′), 2.20 (o, 2H, CH2-2′), 4.54 (t, J = 6.5 Hz, 2H, CH2-3′), 4.59 (brs, 1H, H-29E), 4.72 (d, J = 1.5 Hz, 1H, H-29Z), 2.98 (td, J = 11.5, 5.0 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.48 (m, 1H, H-2b), 2.24 (m, 1H, H-16b), 2.17 (m, 1H, H-13), 1.67, 1.05, 1.00, 0.95, 0.88 × 2 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.0, 176.0, 164.1, 159.5, 159.3, 154.8, 150.5, 121.1, 109.9, 63.5, 60.4, 56.7, 55.2, 50.1, 49.5, 47.5, 47.1, 42.6, 40.8, 39.8, 38.5, 37.1 × 2, 34.3, 33.8, 32.2, 30.8, 29.8, 28.3, 26.8, 25.7, 21.6, 21.2, 19.8, 19.5, 16.1, 15.9, 14.8. HRMS (ESI) m/z: [M + H]+ 619.4107, calculated for C38H55N2O5 619.4111.

2.1.6.6 (Pyridazin-2-acyloxy)-propyl-3-oxolup-20(29)-en-28-oate (BoA3G)

BoA3G was obtained as light-yellow powder; Yield: 44.19%; m. p. 68–71°C. 1H NMR (500 MHz, CDCl3) δ 9.67 (d, J = 1.5 Hz, 1H, H-3″), 9.45 (dd, J = 5.0, 1.0 Hz, 1H, H-6″), 8.00 (dd, J = 5.0, 2.0 Hz, 1H, H-5″), 4.25 (m, 2H, CH2-1′), 2.19 (m, 2H, CH2-2′), 4.51 (t, J = 6.5 Hz, 2H, CH2-3′), 4.59 (brs, 1H, H-29E), 4.72 (d, J = 1.5 Hz, 1H, H-29Z), 2.98 (td, J = 11.0, 5.0 Hz, 1H, H-19), 2.38 (m, 1H, H-2a), 2.48 (m, 1H, H-2b), 2.23 (m, 1H, H-16b), 2.16 (m, 1H, H-13), 1.67, 1.05, 1.00, 0.96, 0.88 × 2 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.0, 176.0, 163.8, 152.2, 150.4, 149.7, 127.8, 125.6, 109.9, 63.3, 60.2, 56.8, 55.2, 50.1, 49.6, 47.5, 47.1, 42.6, 40.8, 39.8, 38.6, 37.1 × 2, 34.3, 33.8, 32.2, 30.7, 29.8, 28.3, 26.8, 25.7, 21.6, 21.2, 19.8, 19.5, 16.1, 16.0, 14.8. HRMS (ESI) m/z: [M + H]+ 619.4113, calculated for C38H55N2O5 619.4111.

2.1.6.7 (Pyrazin-2-acyloxy)-butyl-3-oxolup-20(29)-en-28-oate (BoA4A)

BoA4A was obtained as colorless oil; Yield: 86.57%; m. p. 69–71°C. 1H NMR (500 MHz, CDCl3) δ 9.31 (s, 1H, H-3″), 8.77 (d, J = 2.5 Hz, 1H, H-5″), 8.74 (brs, 1H, H-6″), 4.14 (m, 2H, CH2-1′), 1.71 (m, 2H, CH2-2′), 1.81 (o, 2H, CH2-3′), 4.49 (t, J = 6.5 Hz, 2H, CH2-4′), 4.59 (s, 1H, H-29E), 4.72 (s, 1H, H-29Z), 2.99 (m, 1H, H-19), 2.38 (m, 1H, H-2a), 2.46 (m, 1H, H-2b), 2.25 (m, 1H, H-16b), 2.21 (m, 1H, H-13), 1.67, 1.05, 1.00, 0.96, 0.92, 0.89 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.4, 176.1, 164.0, 150.6, 147.8, 146.4, 144.6, 143.5, 109.8, 65.9, 63.4, 56.6, 55.0, 50.0, 49.4, 47.5, 47.1, 42.6, 40.7, 39.7, 38.4, 37.1, 37.0, 34.3, 33.7, 32.2, 30.7, 29.7, 26.7, 25.6 × 2, 25.5, 21.5, 21.1, 19.7, 19.5, 16.1, 15.9, 14.7. HRMS (ESI) m/z: [M + H]+ 633.4271, calculated for C39H57N2O5 633.4267.

2.1.6.8 (Pyrimidin-2-acyloxy)-butyl-3-oxolup-20(29)-en-28-oate (BoA4F)

BoA4F was obtained as colorless oil; Yield: 77.69%; 1H NMR (500 MHz, CDCl3) δ 9.42 (s, 1H, H-2″), 9.00 (d, J = 4.0 Hz, 1H, H-6″), 8.02 (d, J = 3.5 Hz, 1H, H-5″), 4.14 (m, 2H, CH2-1′), 1.70 (m, 2H, CH2-2′), 1.80 (m, 2H, CH2-3′), 4.48 (t, J = 6.5 Hz, 2H, CH2-4′), 4.58 (brs, 1H, H-29E), 4.71 (d, J = 1.5 Hz, 1H, H-29Z), 2.99 (td, 1H, J = 11.0, 5.0 Hz, H-19), 2.38 (m, 1H, H-2a), 2.47 (m, 1H, H-2b), 2.25 (m, 1H, H-16b), 2.21 (m, 1H, H-13), 1.67, 1.05, 1.00, 0.95, 0.91, 0.87 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.0, 176.1, 164.1, 159.5, 159.2, 154.9, 150.5, 121.1, 109.8, 66.3, 63.3, 56.7, 55.2, 50.1, 49.5, 47.5, 47.1, 42.6, 40.8, 39.8, 38.5, 37.1 × 2, 34.3, 33.8, 32.2, 30.8, 29.8, 26.8, 25.7, 25.6 × 2, 21.6, 21.2, 19.8, 19.5, 16.1, 16.0, 14.8. HRMS (ESI) m/z: [M + H]+ 633.4273, calculated for C39H57N2O5 633.4267.

2.1.6.9 (Pyridazin-2-acyloxy)-butyl-3-oxolup-20(29)-en-28-oate (BoA4G)

BoA4G was obtained as light yellow oil; Yield: 73.80%; 1H NMR (500 MHz, CDCl3) δ 9.67 (d, J = 1.0 Hz, 1H, H-3″), 9.43 (dd, J = 5.0, 1.0 Hz, 1H, H-6″), 7.99 (dd, J = 5.0, 2.0 Hz, 1H, H-5″), 4.15 (m, 2H, CH2-1′), 1.71 (m, 2H, CH2-2′), 1.80 (m, 2H, CH2-3′), 4.45 (t, J = 6.5 Hz, 2H, CH2-4′), 4.59 (s, 1H, H-29E), 4.72 (s, 1H, H-29Z), 2.99 (td, 1H, J = 11.5, 5.0 Hz, H-19), 2.38 (m, 1H, H-2a), 2.48 (m, 1H, H-2b), 2.25 (m, 1H, H-16b), 2.21 (m, 1H, H-13), 1.67, 1.05, 1.00, 0.96, 0.92, 0.89 (s, each 3H, 6 × −CH3). 13C NMR (125 MHz, CDCl3) δ 218.0, 176.1, 163.8, 152.2, 150.5, 149.7, 128.0, 125.5, 109.9, 66.1, 63.2, 56.7, 55.2, 50.1, 49.5, 47.5, 47.2, 42.7, 40.8, 39.8, 38.6, 37.2, 37.1, 34.3, 33.8, 32.2, 30.8, 29.8, 26.8, 25.7, 25.6 × 2, 21.6, 21.2, 19.8, 19.5, 16.1, 16.0, 14.8. HRMS (ESI) m/z: [M + H]+ 633.4271, calculated for C39H57N2O5 633.4267.

2.2 Biology evaluation

2.2.1 Cell culture

The human hepatocellular carcinoma cell line (HepG2), human hepatocellular carcinoma cell line (Bel-7402), human cervical cancer cell line (Hela), human breast adenocarcinoma cell line (MCF-7) and Madin-Darby canine kidney cell line (MDCK) were obtained from the Chinese Academy of Medical Sciences and Peking Union Medical College. Cultures were maintained as monolayer in RPMI-1640/DMEM supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin/streptomycin (Thermo Technologies, New York, NY, United States) under a humidified atmosphere containing 5% CO2 at 37°C. The BoA derivatives and positive control drugs (BoA and DDP) under study were dissolved in DMSO (Sigma, St. Louis, MO, United States) and added at required concentrations to the cell culture. Cells incubated without the preparations served as the control.

2.2.2 Antitumor activity

The antitumor activity was assessed by MTT assay, as previously reported (Fang et al., 2018). Each tested compound and positive control drugs were dissolved in DMSO and diluted with the medium to the test concentrations, and the final concentration of DMSO in the culture medium was controlled at less than 0.5% (v/v). Briefly, cells were cultured at 37°C and dispersed in replicates in 96-well plates with HepG2, Bel-7402, HeLa, MCF-7 and MDCK for 24 h. Fresh medium along with compounds (BoAA-BoAE, BoA2A-BoA2G, BoA3A-BoA3G, BoA4A-BoA4G) at different concentrations was then added to individual wells and incubated for 72 h, with BoA and DDP as the positive control. Experiments on FXR expression were separated into three groups: Control (PBS), BoA2C (8 μM), and BoA2C (8 μM) + DY268 (10 μM) (Giordano et al., 2016), with three repeating wells in each. In separate wells, BoA2C and DY268 were introduced and cultured for 72 h. After 72 h, the cell was incubated with MTT solution (0.5 mg/ml) for an additional 4 h at 37°C. The produced formazan crystals were solubilized with DMSO, and the optical density of solution was measured at 570 nm using a plate reader (BIORAD 550 spectrophotometer, Bio-rad Life Science Development Ltd., Beijing, China). Wells without drugs were used to be blanks. The IC50 values were defined as the concentration of compounds that produced a 50% proliferation inhibition of surviving cells and calculated using the Graph Pad Prism 5. The inhibitory rate was calculated in the following Equation 1, where OD is the optical density:

2.2.3 DAPI staining

Morphological observation of nuclear changes was performed by DAPI staining in this assay. MCF-7 cells in the logarithmic growth phase were seeded in 12-well plates at a density of 5 × 103 cells/well and were allowed to grow for 24 h. Then the cells were treated with different concentration ranges of BoA2C (0, 2, 4 8 μM) for 72 h. After the treatment period, cells were washed with PBS and fixed with 4% paraformaldehyde for 10 min. Then the liquid was discarded, and the cells were stained with DAPI (1 mg/ml, Molecular Probes/Invitrogen Life Technologies, Carlsbad, CA, United States) for 1 min in dark. After staining, cells were visualized under a fluorescence microscope (Olympus IX71, Tokyo, Japan).

2.2.4 Wound healing assay

MCF-7 cells were seeded in six-well plates at a density of 2 105 cells/ml and incubated at 37°C to achieve 100 percent confluence. After starving the cells for 24 h, a wound (cell-free) was drawn across the center of the well using the tip of a 1 ml plastic pipette. Wells were cleaned twice with PBS before being treated for 48 h with BoA2C (8 μM). At 0 h, 24 h, and 48 h, cells were viewed and photographed in randomly selected fields using an inverted light microscope (Nikon).

2.2.5 Cellular metabolomics study

To eliminate excess medium, cells were washed twice with 1 ml of PBS solution. The wells were then filled with 2 ml of ACN: MeOH: H2O 50:30:20 (v/v) and placed in the freezer for 20 min. The extracts obtained in an Eppendorf tube after the cells were scraped and vortexed. The mixture was then centrifuged for 10 min at 10,000 rpm and 4°C, and the supernatant was collected and dried with an evaporator. 300 μl MeOH was used for reconstitution. 15 μL of the reconstituted extract was mixed with 15 μl H2O and 70 L ACN for the metabolomics analysis of the intracellular material. After centrifugation (10 min, 4°C, 11,800 g), the supernatant was transferred to an LC-MS vial for analysis.

An Acquity UPLC HSS T3 column (1.8 μm 100 × 2.1 mm) using an UltiMate 3000 high performance LC system coupled to Q Exactive MS was used for metabolic profiling (Shu et al., 2020). At a flow rate of 0.3 ml/min, mobile phase A (water containing 0.1% acid) and mobile phase B (methanol) were used. A was reduced from 80 % to 70% from 0 to 2 min, A was reduced to 55% from 2 to 5 min, A was reduced to 40% from 5 to 6.5 min, A was reduced to 35% from 6.5 to 12 min, A was last reduced to 0 from 12 to 18 min. The gradient of the mobile phase was as follows: The column oven temperature was set to 40°C, and the autosampler temperature was set to 4°C. The injection volume was 1 μl. MS used electron spray ionization to operate in both positive and negative ionization modes (ESI). To assess the stability and repeatability of the LC-MS-based metabolomics method, quality control (QC) samples were used. Each QC sample was made by combining five samples of the same volume (100 μL) in the order of analysis. To ensure the system method’s stable operation, all combined QC samples for running all QC samples made by every five samples before analysis. After five samples were tested during the analysis run, the combined QC samples of the five samples were taken.

2.3 Statistical analysis

The data were expressed as mean ± SD. Statistical testing using GraphPad Prism 8.0.2. The observed data were compared and analyzed by one-way ANOVA. The levels of statistical significance were set at ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.

3 Results and discussion

3.1 Chemistry

The designed derivatives were prepared following the procedures in Scheme 1. The compound BoA was synthesized from botulin according to the route depicted in Scheme 1, as in detail shown previously (Krasutsky, 2006). In Scheme 1, BoAA and BoAB were synthesized through a combination of BoA and chloromethyl pyrazine in N, N-dimethylformamide (DMF) containing K2CO3 at room temperature, and the yield was 49%–62%. And BoAE was obtained by the same synthetic route at 85°C instead of at room temperature with 82% yield. In addition, methyl-2-pyrazinylmethanol in dry dichloromethane (DCM) containing dichlorosulfoxide (SOCl2) was allowed to stir at room temperature, which was further reacted with BoA in dry DMF in the presence of K2CO3 to yield compounds BoAC and BoAD.

SCHEME 1

SCHEME 1

Synthesis of the BoA and its derivatives BoAA-BoAE. Reagents and Conditions: (A) K2Cr2O7, Al2O3, H2SO4, Acetone, H2O, rt., 3 h; (B) K2CO3, DMF, rt., 2 h; (C) (i) SOCl2, CH2Cl2, rt., 2 h; (ii) K2CO3, DMF, rt., 2 h; (D) K2CO3, DMF, 85°C, 4 h; (E) K2CO3, DMF, rt., 4 h; (F) CH2Cl2, EDCI, DMAP, rt., 4 h.

As seen in Scheme 1, the intermediates BoAnBr/BoAnOH (n = 2, 3, 4) were obtained through a combination of BoA and 1, 2-dibromoethane/1, 3-dibromopropane/1, 4-dibromobutane/2-bromoethanol/3-bromo-1-propanol/4-chloro-1-butanol containing K2CO3 at room temperature in DMF. It is worth mentioning that dihalogenated carbon chain should be 3–4 times as much as BoA when it was reacted with BoA in order to avoid the formation of dipolymer. The compounds BoAnB-BoAnE (n = 2, 3, 4) were produced with 60%–80% yield through a combination of the intermediates BoAnBr (n = 2, 3, 4) and methyl-2-pyrazinic acid by the same synthetic route of BoAE. Interestingly, the compounds BoAnA, BoAnF-BoAnG (n = 2, 3, 4) were synthesized through the combination of the intermediates BoAnOH (n = 2, 3, 4) and 2-pyrazinic acid/4-pyrimidinic acid/4-pyridazinic acid using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI) and 4-dimethylaminopyridine (DMAP) in dry DCM. All reactions were carried out as detailed in the experimental section, and the structures of all target derivatives (Table 1) were confirmed by spectral (1H NMR, 13C NMR and HRMS) analysis (spectra data can be found in Supplementary Materials).

TABLE 1

CompoundnRCompoundnR
BoAA1BoA3B3
BoAB1BoA3C3
BoAC1BoA3D3
BoAD1BoA3E3
BoAE1BoA3F3
BoA2A2BoA3G3
BoA2B2BoA4A4
BoA2C2BoA4B4
BoA2D2BoA4C4
BoA2E2BoA4D4
BoA2F2BoA4E4
BoA2G2BoA4F4
BoA3A3BoA4G4

Structures of the BoA derivatives.

3.2 Biological screening

3.2.1 Cytotoxicity assay

The in vitro cytotoxicity of BoA derivatives was evaluated on four tumor cell lines (HepG2, Bel-7402, Hela, MCF-7) and MDCK cell line using the standard MTT assay. The IC50 values of these compounds were summarized in Table 2. The results revealed that the majority of the compounds considerably increased cytotoxicity in all tumor cell lines examined compared to the BoA, while they showed lower cytotoxicity than DDP on MDCK cell line (13.69 ± 1.45 μM). Among the compounds, BoA3G possessed pronounced cytotoxicity against the cell lines Hela at the IC50 levels of 4.00 ± 0.32 μM (Figure 3). It's worth noting that the most active compound was BoA2C, which revealed more severe cytotoxicity against Bel-7402 and MCF-7 cells at the IC50 levels of 3.65 ± 0.43 μM and 3.39 ± 0.07 μM, comparing with the cisplatin (DDP) against those cells at the IC50 levels of 8.86 ± 1.32 μM and 6.98 ± 0.32 μM, respectively. Meanwhile, BoA2C exhibited lower cytotoxic against MDCK cell (IC50 > 30 μM) than DDP (Figure 4).

TABLE 2

Half maximal inhibitory concentration (IC50) values (µM)
CompoundHepG2Bel-7402HelaMCF-7MDCK
BoAA13.47 ± 1.808.16 ± 2.0818.27 ± 1.6315.66 ± 0.55>30
BoAB11.08 ± 0.277.73 ± 1.9211.80 ± 0.5810.02 ± 0.26>30
BoAC8.95 ± 4.768.24 ± 2.4311.91 ± 1.058.94 ± 1.30>30
BoAD5.17 ± 1.337.51 ± 1.4010.69 ± 0.328.24 ± 0.87>30
BoAE-a----
BoA2A13.73 ± 0.258.76 ± 0.8424.97 ± 0.979.86 ± 0.36>30
BoA2B13.44 ± 1.8210.06 ± 1.4015.48 ± 0.81>30>30
BoA2C7.46 ± 1.433.65 ± 0.4315.59 ± 2.633.39 ± 0.07>30
BoA2D7.32 ± 2.888.22 ± 0.4314.93 ± 1.848.32 ± 1.28>30
BoA2E-----
BoA2F>30>3015.20 ± 1.0729.65 ± 0.3712.50 ± 1.22
BoA2G>3024.81 ± 2.435.81 ± 0.24>3012.97 ± 0.46
BoA3A4.82 ± 0.306.09 ± 0.1220.41 ± 0.566.85 ± 1.82>30
BoA3B13.16 ± 1.2014.24 ± 2.1015.71 ± 2.1527.88 ± 1.06>30
BoA3C>309.37 ± 1.3129.98 ± 2.6510.02 ± 0.19>30
BoA3D8.65 ± 0.606.42 ± 0.669.50 ± 0.285.60 ± 0.2517.01 ± 3.01
BoA3E20.18 ± 0.5311.21 ± 1.5510.13 ± 0.1529.48 ± 0.89>30
BoA3F>3022.31 ± 5.8521.09 ± 6.66>3017.93 ± 0.82
BoA3G>3025.25 ± 2.444.00 ± 0.3213.44 ± 0.5118.53 ± 0.42
BoA4A12.89 ± 4.009.39 ± 0.7313.82 ± 0.129.13 ± 0.4025.32 ± 4.14
BoA4B21.83 ± 2.463.92 ± 1.1011.47 ± 0.93>30>30
BoA4C22.18 ± 1.765.53 ± 0.4617.87 ± 0.459.88 ± 1.49>30
BoA4D>3016.29 ± 0.9015.74 ± 2.15>30>30
BoA4E>30>30>30>30>30
BoA4F30.23 ± 1.51>3025.86 ± 0.5724.47 ± 5.1727.22 ± 0.25
BoA4G29.27 ± 0.2219.43 ± 1.207.46 ± 1.9912.77 ± 0.1516.93 ± 1.03
BoA>30>3027.04 ± 1.06>30>30
DDPb5.23 ± 1.038.86 ± 1.329.84 ± 2.546.98 ± 0.3213.69 ± 1.45

Inhibition effects of BoA derivatives on the growth of tumor cells and MDCK cells in vitro.

a

“-” was meant that the IC50 values of the compounds can’t be determined accurately because of their poor solubility in DMSO.

b

DDP (cisplatin) was used as positive control.

FIGURE 3

FIGURE 3

Comparison of cytotoxicity between BoAnG (n = 2, 3, 4) and DDP.

FIGURE 4

FIGURE 4

Comparison of cytotoxicity between BoAnC (n = 1, 2, 3, 4) and DDP.

Structure-activity relationships (SAR) investigations revealed that BoA derivatives including diazines have varying selectivity for four distinct human cancer cells, with pyrazine derivatives showing the best selectivity. The amount of methyl groups in pyrazine derivatives is critical, since the anticancer activity drops dramatically as the number of methyl groups increases. Despite their improved anticancer activity, pyrimidine and pyridazine compounds exhibited no selectivity. Besides, it was observed that the compounds with pyridazine had stronger activity on Hela cells, and those with 2, 5-dimethylpyrazine seemed to be more active on Bel-7402 and MCF-7 cells. However, there was no obvious rule that the relationship among the differing length of carbon chain between BoA and diazines and cytotoxic activity against a variety of cancer cell lines. In this study, the most promising compound BoA2C was selected for further analysis to reveal its preliminary mechanism of growth inhibition on MCF-7 cells and MDCK cells.

3.2.2 Analyses of cell staining

To characterize the effects of apoptosis inducted by BoA2C on MCF-7 cell and MDCK cell, the nuclear morphological changes in BoA2C-treated MCF-7 and MDCK were observed by DAPI staining. As shown in Figure 5, the morphology of MCF-7 cells in the negative control was normal, after treated with BoA2C (2, 4, 8 μM) for 72 h, MCF-7 cells showed nuclear morphological changes typical of apoptosis in a dose-dependent manner. Even at low concentration of BoA2C (2 μM), In terms of morphological characteristics, the cells had displayed typical death, such as distinctive condensation, margination of chromatin and nuclear fragmentation. With the increase of the concentration of BoA2C, the characteristic of proliferation inhibition was more and more obvious. When the concentration of BoA2C was raised up to 8 μM, the number of cells decreased drastically, the shape of the cells became irregular, and nuclear fragmentation occurred. In contrast, as seen in Figure 6, MDCK cells did not show a significant nuclear damage with increasing concentration of BoA2C. And these results indicated that BoA2C significantly induced apoptosis in MCF-7 cells but less damaged to MDCK cells.

FIGURE 5

FIGURE 5

Morphological detection of apoptosis using DAPI staining (200×) on MCF-7 cells treated with BoA2C: (A) control group; (B) 2 μM; (C) 4 μM; (D) 8 μM.

FIGURE 6

FIGURE 6

Morphological detection of apoptosis using DAPI staining (100×) on MDCK cells treated with BoA2C: (A) control group; (B) 2 μM; (C) 4 μM; (D) 8 μM.

3.2.3 Metabolomics study in MCF-7 cells treated with BoA2C

Mass spectrometry data of metabolites in MCF-7 cells was used for metabolomics investigation after co-cultivation with BoA2C (2 μM) for 72 h. The effectiveness of the OPLS-DA model to distinguish between the BoA2C and Control groups was assessed using the success criterion of R2 ≥ 0.7 and Q2 ≥ 0.4 (Martin-Blazquez et al., 2019). The current model has an R2 of 0.994 and a Q2 of 0.982, indicating that it is capable of distinguishing between BoA2C and Control samples in Figure 7A. VIP-plots were investigated using the OPLS-DA models to examine important factors that led to the differentiation of the metabolome between the BoA2C and Control groups. Variables having a VIP value greater than 1 were deemed significant contenders. Matching retention time and MS fragmentation patterns from the MetaboAnalyst (version 5.0) database verified the presence of distinct metabolites (Pang et al., 2021). These metabolites were further categorized based on their characteristics, including amino acids, amines, aliphatic acids and bile acids. All differential metabolites were loaded into MetaboAnalyst (version 5.0) for metabolic analysis to further explain the fundamental mechanisms of BoA2C’s cytotoxic impact on breast carcinoma cells. In accordance with the described guidelines, the Homo sapiens pathway library and Fisher’s exact test were used for route enrichment analysis, while relative-betweeness centrality was used for pathway topology analysis (Pang et al., 2021). The impacted metabolic pathways were defined as those with a pathway effect value more than 0.10 and a p value less than 0.05. According to the p and pathway impact values, 15 metabolic pathways were observed in BoA2C and Control groups (Figure 7B). Six of them (Figure 7B, Table 3) were primarily involved: 1) arginine and proline metabolism 2) linoleate metabolism 3) bile acid biosynthesis 4) de novo fatty acid biosynthesis 5) fatty acid activation, and (VI) fatty Acid Metabolism.

FIGURE 7

FIGURE 7

Metabolomics study for BoA2C in MCF-7 cells. (A) OPLS-DA score plots of BoA2C and control group. (B) KEGG pathway enrichment analysis of differential metabolites between BoA2C and control group.

TABLE 3

nonamemolecular formulaadductobserved massexact massMass error (ppm)rt (min)VIP valuefold changep valuemetabolic pathway
M1CholesterolC27H46O[M-H2O + H] +369.3508386.3548-1.916.5810.047.692.20 × 10−4Bile acid biosynthesis
M2Cholic acidC24H40O5[M-H2O + H] +391.2834408.2875-2.016.329.222.554.05 × 10−3Bile acid biosynthesis
M33 alpha,7 alpha,26-Trihydroxy-5beta-cholestaneC27H48O3[M + Na] +443.3513420.36034.113.163.89362.981.58 × 10−13Bile acid biosynthesis
M44-Acetamidobutanoic acidC6H11NO3[M-H2O + H] +128.0704145.0738-0.81.523.642.373.61 × 10−5Arginine and Proline Metabolism
M5L-ProlineC5H9NO2[M + H] +116.0704115.0633-1.71.292.992.408.91 × 10−9Arginine and Proline Metabolism
M6L-4-Hydroxyglutamate semialdehydeC5H9NO4[M + H] +148.0601147.0531-2.01.292.951.993.89 × 10−11Arginine and Proline Metabolism
M7L-GlutamineC5H10N2O3[M + H] +147.0762146.0691-1.41.312.922.332.66 × 10−10Arginine and Proline Metabolism
M8LysoPC(P-18:0/0:0)C26H54NO6P[M + H] +508.3757507.3688-0.811.842.873.293.82 × 10−9Linoleate metabolism
M913-HpodeC18H32O4[M + Na] +335.2210312.23015.16.352.7423.041.68 × 10−11Linoleate metabolism
M105′-MethylthioadenosineC11H15N5O3S[M + H] +298.0962297.0895-2.01.542.7328.551.92 × 10−12Arginine and Proline Metabolism
M11Palmitic acidC16H32O2[M + Na] +279.2312256.24026.411.182.5323.171.08 × 10−11De novo fatty acid biosynthesis
M12L-MethionineC5H11NO2S[M + H] +150.0581149.0510-1.31.322.423.631.41 × 10−8Arginine and Proline Metabolism
M133a,7a-Dihydroxy-5b-cholestan-26-alC27H46O3[M + Na] +441.3364418.34465.914.932.28241.464.33 × 10−10Bile acid biosynthesis
M14L-ArginineC6H14N4O2[M + H] +175.1187174.1116-1.10.972.221.901.39 × 10−8Arginine and Proline Metabolism
M153-Oxotetradecanoyl-CoAC35H60N7O18P3S[M-H2O + H] +974.2870991.2928-2.61.302.081.774.88 × 10−8Fatty Acid Metabolism
M167a,12a-Dihydroxy-cholestene-3-oneC27H44O3[M + H] +417.3356416.329-1.711.421.9217.048.24 × 10−11Bile acid biosynthesis
M177alpha-HydroxycholesterolC27H46O2[M + Na] +425.3411402.34975.213.281.8792.351.78 × 10−8Bile acid biosynthesis
M18L-Glutamic acidC5H9NO4[M-H2O + H] +130.0497147.0531-0.81.361.861.943.80 × 10−7Arginine and Proline Metabolism
M19Ascorbic AcidC6H8O6[M-H2O + H] +159.0274176.0321-8.81.191.722.888.29 × 10−10Linoleate metabolism
M20Linoleic acidC18H32O2[M + Na] +303.2311280.24025.612.831.57159.587.93 × 10−12Linoleate metabolism
M213,7-Dihydroxycoprostanic AcidC27H46O4[M + Na] +457.3314434.33965.711.821.541027.396.53 × 10−12Bile acid biosynthesis
M222-trans-dodecenoyl-CoAC33H56N7O17P3S[M + H] +948.2762947.26662.41.371.511.934.45 × 10−5Fatty Acid Metabolism
M2313-HODEC18H32O3[M + H] +315.2522296.2351-2.58.551.43108.547.47 × 10−10Linoleate metabolism
M2427-Deoxy-5b-cyprinolC27H48O4[M + Na] +459.3459436.35523.33.281.432.032.47 × 10−3Bile acid biosynthesis
M257a-Hydroxy-5b-cholestan-3-oneC27H46O2[M + Na] +425.3403402.34973.316.171.309.401.06 × 10−7Bile acid biosynthesis
M26alpha-Linolenic acidC18H30O2[M + H2O + H] +297.2417278.2245-2.413.591.272.192.42 × 10−9De novo fatty acid biosynthesis
M27Lithocholic acidC24H40O3[M-H2O + H] +359.2935376.2977-2.514.881.26108.494.27 × 10−10Bile acid biosynthesis
M287a-Hydroxy-cholestene-3-oneC27H44O2[M-H2O + H] +383.3299400.3341-2.310.751.154.477.13 × 10−4Bile acid biosynthesis

Potential biomarker from primarily involved six metabolic pathways.

Arginine is a type of multi-functional amino acid involved in the synthesis of many metabolites, such as nitric oxide, urea, ornithine, and citrulline, and is involved in protein modification and immunoregulation (Morris, 2007; Rath et al., 2014; Szefel et al., 2019). ASS1, one of the key enzymes involved in arginine metabolism, is highly expressed in normal tissues but heterogeneously expressed in tumors (Dillon et al., 2004). These researchers found that ASS1 was completely deficient in HCC and prostate cancer and partially deficient in breast cancer (Dillon et al., 2004; Morettin et al., 2015). Several recent investigations have shown that arginine deprivation caused tumor cell death via degrading extracellular arginine (Alexandrou et al., 2018; Nasreddine et al., 2020; Chu et al., 2021). This suggested that arginine shortage might occur in MCF-7 cells treated with BoA2C based on the noticeably changed in arginine metabolism. Bile acid composition is controlled via the modulation of bile acid-metabolizing enzymes and bile acid transporters, the majority of which are expressed by farnesoid X receptor (FXR) target genes (Sun et al., 2021). FXR regulates the expression of target genes involved in both bile acid production and fatty acid biosynthesis, which is required for the maintenance of whole-body bile acid homeostasis (Matsubara et al., 2013; Chiang and Ferrell, 2020). Furthermore, FXR stimulation weakened cell proliferation and migration under steroid-free media conditions in MCF-7 and T47D breast cancer cells, and other investigators observed that FXR agonists caused apoptosis and inhibited local estrogen synthesis (Alasmael et al., 2016; Giordano et al., 2016; Barone et al., 2018). As shown in Figure 8A, the scratch wounds and migratory cells in the wound were imaged at 0, 24, and 48 h after scratched in this study. The results demonstrated that the treatment of BoA2C obviously reduced the migration of MCF-7 cells in the scratch compared to control group, thus potentially inhibited tumor invasion with enhanced FXR expression. Then, a potent FXR antagonist (DY268) was employed (Yu et al., 2014; Hankir et al., 2020) in a cell proliferation investigation to better evaluate the regulatory link between FXR expression and BoA2C treatment. The results (Figure 8B) unequivocally demonstrated that DY268 reversed BoA2C inhibitory impact on the growth of MCF-7 breast cancer cells in both 24, 48 and 72 h. Thus, BOA2C could disrupt bile acid and fatty acid metabolism and limit tumor development and invasion probably by activating FXR expression.

FIGURE.8

FIGURE.8

The effect of BoA2C on the invasive ability of MCF-7 cells and the role of FXR in the anticancer process. (A) Wound healing assays were performed in 0, 24, 48 h; (B) Changes of antiproliferative activity under DY268. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. control cells.

4 Conclusion

In this study, 26 new betulonic acid-diazines were designed and synthesized from BoA and all of them were characterized by 1H NMR, 13C NMR, HRMS. Furthermore, all these compounds were tested for cytotoxic activity against HepG2, Bel-7402, Hela, MCF-7 and MDCK cell lines by standard MTT assay. After exploring the structure-activity relationship, we found that the introduction of different groups at C-28 can selectively inhibit different kinds of cancer cells. The introduction of a pyridazine structure at the C-28 of BoA was possessed a stronger cytotoxic activity against Hela cell. It is interesting that the introduction of a 2, 5-dimethylpyrazine structure might be more effective cytotoxicity against Bel-7402 and MCF-7 cells and lower cytotoxicity on MDCK than DDP. From the obtained results, compounds BoA2C exerted potent and selective in vitro antiproliferative activity. As shown in metabolomics results, it was found that BoA2C significantly induced cell injury in MCF-7 cells mostly through the interference of arginine metabolism and fatty acid metabolism. It’s presently undertaking intense adjustments at C-28 and investigations in the mechanism of BoA2C’s antiproliferative impact in our further studies, and the results will be revealed in due course.

Statements

Data availability statement

The original contributions presented in the study are included in the article/Supplementary Material, further inquiries can be directed to the corresponding authors.

Author contributions

YS, Investigation, Methodology, Formal analysis, Writing—Original draft preparation. FL, Formal analysis, Supervision, Writing—original draft preparation. YH, Investigation, Formal analysis, Methodology. PW, Reviewing andamp, editing, Supervision, Validation. FG, Formal analysis, Editing. MY, Methodology. ML, Methodology. QM, Methodology. YZ, Reviewing andamp; editing. XD, Conceptualization, Supervision. HL, Conceptualization, Supervision, Validation, Funding cquisition, Project administration.

Funding

This research was supported by the 111 Project B21028, the Fundamental Research Funds for the Central Universities (2019-JYB-TD005, China), Beijing Key Laboratory for Basic and Development Research on Chinese Medicine (Beijing, 100102).

Conflict of interest

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Publisher’s note

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.

Supplementary material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fchem.2022.969770/full#supplementary-material

References

Summary

Keywords

betulonic acid-diazine derivatives, nitrogen-containing heterocycles, synthesis, cytotoxicity, metabolomics

Citation

Shu Y, Li F, Han Y, Wang P, Gao F, Yan M, Liang M, Ma Q, Zhang Y, Ding X and Lei H (2022) Design, synthesis and cytotoxic evaluation of novel betulonic acid-diazine derivatives as potential antitumor agents. Front. Chem. 10:969770. doi: 10.3389/fchem.2022.969770

Received

15 June 2022

Accepted

29 July 2022

Published

06 September 2022

Volume

10 - 2022

Edited by

Cheng-Tao Feng, Anhui University of Chinese Medicine, China

Reviewed by

Gang Li, Chinese Academy of Medical Sciences and Peking Union Medical College, China

Xiaodong Ma, Anhui University of Chinese Medicine, China

Updates

Copyright

© 2022 Shu, Li, Han, Wang, Gao, Yan, Liang, Ma, Zhang, Ding and Lei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Qiang Ma, ; Yuzhong Zhang, ; Xia Ding, ; Haimin Lei ,

†These authors have contributed equally to this work and share first authorship

This article was submitted to Organic Chemistry, a section of the journal Frontiers in Chemistry

Disclaimer

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

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