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General Commentary ARTICLE

Front. Plant Sci., 16 February 2016 | https://doi.org/10.3389/fpls.2016.00158

Commentary: Plant Auxin Biosynthesis Did Not Originate in Charophytes

Chunyang Wang1,2, Si-Shen Li1* and Guan-Zhu Han2*
  • 1State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, China
  • 2Jiangsu Key Laboratory for Microbes and Functional Genomics, Jiangsu Engineering and Technology Research Center for Microbiology, College of Life Sciences, Nanjing Normal University, Nanjing, China

A commentary on
Plant auxin biosynthesis did not originate in charophytes

by Turnaev, I., Gunbin, K., and Afonnikov, D. (2015). Trends Plant Sci. 20, 463–465. doi: 10.1016/j.tplants.2015.06.004

The TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) family of aminotransferases and the YUCCA (YUC) family of flavin monooxygenases are required for the biosynthesis of auxin (Mashiguchi et al., 2011). However, the origin of TAA-YUC auxin biosynthesis pathway is under hot debate recently (Wang et al., 2014; Yue et al., 2014; Turnaev et al., 2015). By similarity searches and phylogenetic analyses, Yue et al. did not find TAA and YUC homologs in any algal group (Yue et al., 2014). On the contrary, we found TAA and YUC protein homologs in the genome of Klebsormidium flaccidum, a charophyte alga, and proposed that plant auxin biosynthesis might originate in charophytes (Wang et al., 2014). More recently, Turnaev et al. (2015) reanalyzed the structures and phylogenetic relationship of TAA family proteins and claimed K. flaccidum TAA-like protein (kfl00051_0080) is more closely related to alliinases than tryptophan aminotransferases. However, we believe this represents a common misinterpretation of phylogenetic tree, which leads to erroneous inferences of ancestry and evolutionary relationship.

First, Turnaev et al. missed several TAA protein homologs in Choanoflagellida. We employed the BLASTP algorithm with Arabidopsis thaliana TAA1 protein as the query and found significant hits in Salpingoeca rosetta [XP_004988548, e = 10−37, identity = 97/317 (31%)] and Monosiga brevicollis [XP_001746485, e = 10−34, identity = 86/290 (30%)]. Also, Turnaev et al. missed many TAA protein homologs in bacteria [e.g., WP_012083818 of Sulfurovum sp., e = 2 × 10−8, identitiy = 75/302 (25%)] and archaea [e.g., WP_006652091 of Natrialba hulunbeirensis, e = 9 × 10−7, identity = 82/323 (25%)]. When the identity of protein pairs is higher than 25%, and the number of residues aligned is higher than 150, evolutionary relatedness could be convincingly inferred (Chung and Subbiah, 1996; Rost, 1999). It appears that these additional eukaryotic and prokaryotic proteins are homologous to A. thaliana TAA1 protein. Missing bacteria and archaea homologs leads to the absence of outgroup taxa to root the TAA phylogenetic tree (Figure 1A). Also, one key node (the clade of land plant alliinases, kfl00051_0080, and the Capsaspora owczarzaki sequence) is weakly supported (posterior probability: 0.463), making the deep relationship of plant and non-plant eukaryote TAA proteins unresolved. However, our phylogenetic tree with more non-plant eukaryote and prokaryote TAA-like proteins is more strongly supported in terms of posterior probability (see Figure 1D in this paper and Figure 1A in Wang et al., 2014).

FIGURE 1
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Figure 1. Simplified unrooted (A) and rooted tree (B–D) of TAA family proteins. For the unrooted tree (A), there are at least three rooted trees (B–D). The orange and green triangles represent land plant tryptophan aminotransferase and alliinase clades respectively. Trees (E,F) are rooted trees with the Haptophyceae and Cryptophyta sequences and the Ichthyosporea sequence as the outgroups, respectively. Trees (B,E) are modified from Turnaev et al. (2015). Tree (D) is modified from Wang et al. (2014).

Most importantly, what Turnaev et al. present is an unrooted phylogenetic tree. Based on the unrooted phylogenetic tree, they claimed the kfl00051_0080 from K. flaccidum belongs to the land plant alliinase clade. Unfortunately, this claim results from a misinterpretation of phylogenetic tree, i.e., confusion between unrooted tree and rooted tree. For an unrooted tree, the common ancestor to all the taxa is not identified. The root can be placed along any of the branches of an unrooted tree. For an unrooted tree, there are 2T-3 possible rooted trees, where T represents the number of taxa within the tree. To be simple, let's ignore the branches within land plants and the nodes of non-plant eukaryotes (the key node is not well supported; Figure 1A). Without a prior knowing of where the root is, the simplified unrooted phylogenetic tree of TAA proteins can be “transformed” into three rooted trees (Figures 1B–D); it should be noted that only one of them is correct. Turnaev et al. arbitrarily took an unrooted tree as a rooted tree and concluded that K. flaccidum TAA protein is more closely related to plant alliinases (Figure 1B). Even if we assume Turnaev et al.'s tree is a rooted tree with Cryptophyta and Haptophyceae as the outgroups (Figure 1E). Then the problem is why they used Cryptophyta and Haptophyceae sequences as the outgroups, but not Ichthyosporea sequence (Figure 1F). If we use the Ichthyosporea sequence as the outgroup, the K. flaccidum kfl00051_0080 is basal to land plant alliinases and tryptophan aminotransferases (Figure 1F). Nevertheless, our phylogenetic analysis with bacterial and archaeal sequences reveals where the root is (see Figure 1D in this paper and Figure 1A in Wang et al., 2014). It appears that the K. flaccidum kfl00051_0080 is equally related to land plant alliinases and tryptophan aminotransferases.

Based on the phylogenetic analysis, Turnaev et al. (2015) conclude “land plants and the ancestor of K. flaccidum acquired TAA-like and alliinase genes independently through a horizontal gene transfer from non-plant taxa.” Again this claim is based on an unrooted tree. The directionality of their phylogenetic tree is unknown and thus the claim is not verified. Even we assume that Turnaev et al.'s tree is rooted as discussed above, there are not two HGT events from non-plant taxa, because the most parsimonious explanation of Ichthyosporea sequence should be HGT from plant to Ichthyosporea. Our phylogenetic analysis with bacteria and archaea clearly shows that all the alliinase/aminotransferase genes originated from a single horizontal gene transfer event (Wang et al., 2014). Our explanation (one gain) is more parsimonious than Turnaev et al.'s (scenario 1: two independent HGT events; scenario 2: one gain, one duplication, one loss, etc.).

The two clades of land plant TAA family proteins arose from an ancient gene duplication event that occurred after the divergence of K. flaccidum from plants but before/around the emergence of land plants. In terms of functionality origin and evolution, there are at least two possible trajectories, subfunctionalization (the proto-gene represented by kfl0051_0080 has both functions) and neofunctionalization (either alliinase or aminotransferase function is newcomer). However, these hypotheses are based on the assumption that the so-called alliinases do not participate in the biosynthesis of auxin, which we still do not know. It is likely that both clades of TAA family proteins play a role in the auxin biosynthesis, since the core residues of substrate binding are conserved among tryptophan aminotransferases and alliinases of land plants and K. flaccidum (Figure S1; He et al., 2011). We agree that further experimental work could resolve the controversy.

Nevertheless, this case might point to a general problem when researchers from outside the field of evolutionary biology come into phylogenetic analysis. Nowadays phylogenetic analysis has become more and more frequently used in modern biology, including plant science. How to interpret phylogenetic tree is becoming a challenge (Baum et al., 2005). It should be noted that nearly all the tree inference (Neighbor Joining, Maximum Likelihood, Bayesian, etc.) methods produce unrooted trees (Philippe et al., 2011). Often the tree displayed by software is intuitively taken as rooted tree, which is likely to lead to misinterpretation of phylogenetic tree and errors, especially the common fallacy that taxa are assigned to the wrong clade.

Author Contributions

GH, CW, and SL conceived the study. GH wrote the manuscript.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

This work was supported by the Priority Academic Program Development (PAPD) of Jiangsu Higher Education Institutions and the National Key Technologies R&D Program of China (grant no. 2011BAD35B03).

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/article/10.3389/fpls.2016.00158

References

Baum, D. A., Smith, S. D., and Donovan, S. S. (2005). The tree-thinking challenge. Science 310, 979–980. doi: 10.1126/science.1117727

PubMed Abstract | CrossRef Full Text | Google Scholar

Chung, S. Y., and Subbiah, S. (1996). A structural explanation for the twilight zone of protein sequence homology. Structure 4, 1123–1127. doi: 10.1016/S0969-2126(96)00119-0

PubMed Abstract | CrossRef Full Text | Google Scholar

He, W., Brumos, J., Li, H., Ji, Y., Ke, M., Gong, X., et al. (2011). A small-molecule screen identifies L-kynurenine as a competitive inhibitor of TAA1/TAR activity in ethylene-directed auxin biosynthesis and root growth in Arabidopsis. Plant Cell 23, 3944–3960. doi: 10.1105/tpc.111.089029

PubMed Abstract | CrossRef Full Text | Google Scholar

Mashiguchi, K., Tanaka, K., Sakai, T., Sugawara, S., Kawaide, H., Natsume, M., et al. (2011). The main auxin biosynthesis pathway in Arabidopsis. Proc. Natl. Acad. Sci. U.S.A. 108, 18512–18517. doi: 10.1073/pnas.1108434108

PubMed Abstract | CrossRef Full Text | Google Scholar

Philippe, H., Brinkmann, H., Lavrov, D., Littlewood, D., Manuel, M., Wörheide, G., et al. (2011). Resolving difficult phylogenetic questions: why more sequences are not enough. PLoS Biol. 9:e1000602. doi: 10.1371/journal.pbio.1000602

PubMed Abstract | CrossRef Full Text | Google Scholar

Rost, B. (1999). Twilight zone of protein sequence alignments. Protein Eng. 12, 85–94. doi: 10.1093/protein/12.2.85

PubMed Abstract | CrossRef Full Text | Google Scholar

Turnaev, I., Gunbin, K., and Afonnikov, D. (2015). Plant auxin biosynthesis did not originate in charophytes. Trends Plant Sci. 20, 463–465. doi: 10.1016/j.tplants.2015.06.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Wang, C., Liu, Y., Li, S., and Han, G. (2014). Origin of plant auxin biosynthesis in charophyte algae. Trends Plant Sci. 19, 741–743. doi: 10.1016/j.tplants.2014.10.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Yue, J., Hu, X., and Huang, J. (2014). Origin of plant auxin biosynthesis. Trends Plant Sci. 19, 764–770. doi: 10.1016/j.tplants.2014.10.004

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: auxin biosynthesis, phylogenetics, charophytes, horizontal gene transfer, comparative genomics

Citation: Wang C, Li S-S and Han G-Z (2016) Commentary: Plant Auxin Biosynthesis Did Not Originate in Charophytes. Front. Plant Sci. 7:158. doi: 10.3389/fpls.2016.00158

Received: 16 November 2015; Accepted: 30 January 2016;
Published: 16 February 2016.

Edited by:

Laurent Deluc, Oregon State University, USA

Reviewed by:

Stefan A. Rensing, Philipp University of Marburg, Germany

Copyright © 2016 Wang, Li and Han. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Guan-Zhu Han, guanzhu@email.arizona.edu;
Si-Shen Li, ssli@sdau.edu.cn