Silicon: Potential to Promote Direct and Indirect Effects on Plant Defense Against Arthropod Pests in Agriculture
- 1Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
- 2Graham Centre for Agricultural Innovation, New South Wales Department of Primary Industries, Menangle, NSW, Australia
- 3Proteomics Core Facility, School of Life Sciences, University of Technology Sydney, Sydney, NSW, Australia
- 4College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China
- 5Graham Centre for Agricultural Innovation, Charles Sturt University, Orange, NSW, Australia
Silicon has generally not been considered essential for plant growth, although it is well recognized that many plants, particularly Poaceae, have substantial plant tissue concentrations of this element. Recently, however, the International Plant Nutrition Institute [IPNI] (2015), Georgia, USA has listed it as a “beneficial substance”. This reflects that numerous studies have now established that silicon may alleviate both biotic and abiotic stress. This paper explores the existing knowledge and recent advances in elucidating the role of silicon in plant defense against biotic stress, particularly against arthropod pests in agriculture and attraction of beneficial insects. Silicon confers resistance to herbivores via two described mechanisms: physical and biochemical/molecular. Until recently, studies have mainly centered on two trophic levels; the herbivore and plant. However, several studies now describe tri-trophic effects involving silicon that operate by attracting predators or parasitoids to plants under herbivore attack. Indeed, it has been demonstrated that silicon-treated, arthropod-attacked plants display increased attractiveness to natural enemies, an effect that was reflected in elevated biological control in the field. The reported relationships between soluble silicon and the jasmonic acid (JA) defense pathway, and JA and herbivore-induced plant volatiles (HIPVs) suggest that soluble silicon may enhance the production of HIPVs. Further, it is feasible that silicon uptake may affect protein expression (or modify proteins structurally) so that they can produce additional, or modify, the HIPV profile of plants. Ultimately, understanding silicon under plant ecological, physiological, biochemical, and molecular contexts will assist in fully elucidating the mechanisms behind silicon and plant response to biotic stress at both the bi- and tri-trophic levels.
Silicon and the Soil
Silicon is the second most abundant element, after oxygen, in the Earth’s crust and in the soil solution (Epstein, 1994). It is mainly present in the soil solution in the form of silicic acid, H4SiO4, since this is the only form of water-soluble silicon. Soil concentrations typically range from 0.1 to 0.6 mM (Epstein, 1994). This concentration range is similar to that of major inorganic nutrients including potassium, calcium, and sulfate in the soil solution (Epstein, 1972). Several factors influence soil silicon availability to plants, including soil type, parent material, land use, organic matter, temperature, soil pH, and texture (Liang et al., 1994; Alexandre et al., 1997; Struyf et al., 2010; Cornelis et al., 2011; Han et al., 2011; Miles et al., 2014; Anda et al., 2015).
Silicon and Plants
Silicon is taken up by plants via the transpiration stream (i.e., passive uptake) and is transported from the roots to the shoots as monosilicic acid, where it is deposited as solid, amorphous, hydrated plant silica (SiO2.nH2O; Jones and Handreck, 1967). Once deposited, silicon is not remobilized (Raven, 1983). Silicon is transported in the plant through the xylem via apoplastic transport (Raven, 1983) and must remain in solution (i.e., remain unpolymerized) during this passage; however the mechanisms preventing polymerization are not well understood (Epstein, 1994). Active silicon uptake is exhibited by some plant species including rice Oryza sativa L. (Takahashi et al., 1990; Henriet et al., 2006; Liang et al., 2006), as is rejective uptake (i.e., uptake at rates lower than passive; Takahashi et al., 1990). The existence of these processes indicates that, in some plant taxa at least, plant silicon levels are actively manipulated. Selection pressure for the evolution of active silicon uptake and metabolism is evident in the beneficial effects of silicon to plants under abiotic and biotic stress. However, silicon has not generally been recognized as an essential plant nutrient, though recently the International Plant Nutrition Institute [IPNI] (2015), Georgia, USA listed silicon as a “beneficial substance” (International Plant Nutrition Institute [IPNI], 2015).
The positive effects of silicon against abiotic and biotic stress are not always obvious since the extent of silicon accumulation differs among plant species and cultivars (Deren, 2001; Mitani and Ma, 2005; Keeping and Reynolds, 2009). Terrestrial plants have tissue concentrations of silicon, ranging from 1 to 15% dry weight (Epstein, 1994), with a very irregular distribution among the plant kingdom (Epstein, 1999). In agricultural systems, silicon is applied as a crop protection treatment and this is the major focus of this review. Major crops that respond to silicon application include some monocotyledonous plants such as rice, maize, Zea mays L., and wheat, Triticum aestivum L., that actively absorb and accumulate high amounts of silicon, together with some dicotyledonous crops such as cotton (Gossypium hirsutum L.), soybean [Glycine max (L.) Merr.], some vegetables (e.g., cucurbits) and fruit crops (e.g., tomato (Lycopersicon esculentum Mill.) that accumulate silicon through specific transporters (Liang et al., 2015). While it is well documented that sugarcane responds strongly to silicon fertilization, active absorption of silicon has not been demonstrated and an active transporter has not yet been found. More recently, high-throughput sequencing and easier access to genomic data has enabled accurate determination of the ability of a plant to accumulate silicon, based on its genetic predisposition (Liang et al., 2015).
Until the discovery of specific genes involved in silicon uptake, silicon accumulation in plants was little understood. These silicon transporter genes, influx and efflux (LSi1 and LSi2, respectively), responsible for silicon uptake by the roots were first described in rice (Ma et al., 2006, 2007). Homologs are now reported in barley, Hordeum vulgare L., maize, and wheat (Chiba et al., 2009; Mitani et al., 2009a,b; Montpetit et al., 2012), with pumpkin, Cucurbita moschata, Poir. the first dicot to record a gene encoding a silicon influx transporter, LSi1 (Mitani et al., 2011) and two efflux transporters, CmLSi2-1 and CmLSi2-2 (Mitani-Ueno et al., 2011) followed by two putative influx silicon transporter genes (GmNIP2-1 and GmNIP2-2) in soybean (Deshmukh et al., 2013) and cucumber (CSiT-1, CSiT-2; Wang et al., 2015). An influx transporter has also been identified in the primitive plant, horsetail, Equisetum arvense L. (Gŕegoire et al., 2012). A silicon influx transporter, LSi6, present in the root tips, leaf sheaths and leaf blades has also been identified in several graminaceous species, including rice, and is responsible for xylem unloading of silicon (Yamaji et al., 2008).
Silicon and Stress
The beneficial effects of silicon application on plant growth and crop yield are well documented (for a recent review see Guntzer et al., 2012), but it is in the mitigation of both abiotic and biotic plant stresses, where the application of silicon demonstrates its real potential (Keeping and Reynolds, 2009). Notably, biochemical or molecular responses (and frequently growth/yield responses) due to silicon fertilization, are usually not apparent unless in the presence of a biotic (or abiotic) stressor. Studies have shown resistance to a range of abiotic stress factors including drought and salinity stress, heavy metal toxicity, excess nitrogen and phosphorous, and lodging (for a recent review see Liang et al., 2015). Biotic stressors may come in the form of plant pathogens, including fungi, bacteria, viruses, and animals (vertebrate and arthropod herbivores). Defense against biotic stress, has centered around two main mechanisms, mechanical (physical), and biochemical or molecular.
There is a dominance of work on fungal pathogens, compared with other disease-causing agents. Those fungal pathogens defined as biotrophic or hemibiotrophic, including the powdery mildews and blast fungus (Magnaporthe grisea (T.T. Hebert) M.E. Barr), appear to be better controlled by silicon than are necrotrophs (Liang et al., 2015). The reasons for this are increasingly becoming apparent, with a recent study showing that while silicon contributes to Arabidopsis defense priming following pathogen infection, that silicon will confer protection even when priming is altered, indicating other mechanisms may be involved (Vivancos et al., 2015). Evidence suggests that silicon may interfere with effector proteins released by these pathogens, permitting the plant to mount better defense reactions (Vivancos et al., 2015). Other work has confirmed the role of silicon in priming plants in plant–pathogen interactions (Fauteux et al., 2005; Chain et al., 2009; Van Bockhaven et al., 2013). It is thought that the work on silicon and effector proteins may assist in developing a unifying theory around the mode of action of silicon in alleviating biotic stresses (Vivancos et al., 2015). A recent, comprehensive review of silicon and plant–pathogen interactions in agriculture is provided by Liang et al. (2015).
Vertebrate herbivores are probably the least studied biotic stressors, against which silicon provides some protection, and research in this area has largely focused on natural ecological systems. We briefly review this field because it has some relevance to arthropod pests given that plant defenses are at the heart of the phenomenon. The majority of studies have been on field voles, Microtus agrestis L (Rodentia: Cricetidae), showing reductions in the body weight and growth rate of juveniles and adults when fed on silicon-treated grasses (Massey and Hartley, 2006; Massey et al., 2008). Recent laboratory work demonstrated that grasses employ several defense strategies against M. agrestis including silicon, endophytes, and secondary metabolites (Huitu et al., 2014). It is hypothesized that induction of silicon-based plant defense in response to herbivore damage may influence rodent population cycles (Massey et al., 2008). In sites where M. agrestis population density was high, silica levels in the leaves of their food plant, collected several months later were also high and vole populations afterward declined, while population density increased where vole population density was initially low and silicon levels were also low (Massey et al., 2008). A key food species, Deschampsia cespitosa L., of M. agrestis exhibits a delayed defensive response to grazing by increasing silica concentrations (Reynolds et al., 2012). Further, the authors presented theoretical modeling that predicts that this response alone could lead to population cycles observed in M. agrestis and in other graminivorous rodent populations, where populations that reach sufficiently high densities can induce silica defenses in their food source.
Studies on the root vole, Microtus oeconomus (Pallas, 1776), have shown that changes in the silicon content of tussock sedges may be induced by variations in vole population densities (Wieczorek et al., 2015). However, no correlation was shown between the silicon content in the faeces of M. oeconomus and survival rate (Wieczorek et al., 2015). A very recent study in Poland demonstrated that the amount of silica in plants, fed upon by voles, leaves a traceable record in their dental microwear textures, and that these differ through different phases of vole population cycles (Calandra et al., 2016). The authors hypothesize that the high quantity of phytoliths, produced due to intense grazing in peak years, can result in malocclusion and other dental abnormalities, and may explain how these silicon-based plant defenses contribute to population crashes. Silicon-treated wheat plants showed enhanced resistance to feeding by the wild rabbit (Oryctolagus cuniculus L.), a major vertebrate pest of cereals in the United Kingdom (Cotterill et al., 2007). Further, severe, potentially lethal feeding damage due to rabbit browsing, was reduced in silicon-treated wheat by over 50%. Feeding preference in sheep (Ovis aries L.), in response to silicon availability, did not differ within a grass species; however, there were differences in the bite rate and feeding preference between grass species, with these differences more obvious in silicon-treated plants (Massey et al., 2009). Further, silicon influenced grass preference less in palatable species, compared to less desirable species, an effect that appeared to be due to the most palatable species containing relatively little silicon even after supplementation, and being less tough (Massey et al., 2009).
Numerous studies have shown enhanced resistance of plants treated (soil and/or foliar application) with silicon to insect herbivores and other arthropods, including folivores (Korndorfer et al., 2004; Redmond and Potter, 2006; Massey et al., 2007; Han et al., 2015), borers (Kvedaras and Keeping, 2007; Kvedaras et al., 2007a,b, 2009; Hou and Han, 2010; Keeping et al., 2013; Vilela et al., 2014), phloem (Correa et al., 2005; Goussain et al., 2005; He et al., 2015) and xylem feeders (Yoshihara et al., 1979), mites (Nikpay and Nejadian, 2014) and nematodes (Silva et al., 2015). However, there is no consistent evidence for silicon having a greater effect in any particular feeding guild or taxon (Keeping and Kvedaras, 2008). The vast majority of studies are at two trophic levels, with few studies at the third trophic level (Reynolds et al., 2009; Gurr and Kvedaras, 2010; Kvedaras et al., 2010). A comprehensive review of earlier work on the role of silicon against herbivorous insects was provided by Reynolds et al. (2009).
This paper explores the more recent advances in the role of silicon in ameliorating the effects of biotic stress, particularly that caused by arthropods from agricultural systems, and the response of their natural enemies, together with the mechanisms involved in bi- and tri-trophic interactions. We also review literature relating to the effects of silicon on plant pathogens where this helps illustrate underlying mechanisms of plant defense that may have relevance to arthropods. Understanding the role and function of silicon against arthropod pests, will ultimately enable us to optimize the use of this element in the context of sustainable agriculture.
Silicon fertilization of plants has proven to be effective in controlling insect herbivores and other arthropods. Indeed, silicon application has become a routine practice in rice production in some countries, including Japan, where a silicon fertilizer was first applied to any crop worldwide (Ma and Takahashi, 2002). In agricultural systems, silicon is typically applied to the soil, or as a foliar spray to the vegetation. It is feasible that foliar application of silicon can have an effect on arthropods, e.g., via surface pH or osmotic effects. However, there is now considerable evidence, notably in fungal systems, that soil applied silicon leads to significantly more silicon accumulation in plant tissues, than foliar applications and produces much better results against biotic stressors (Liang et al., 2005, 2015; Guével et al., 2007; Dallagnol et al., 2015). Details of the mechanisms underlying silicon-mediated plant resistance against biotic stress are increasingly becoming clear, with an increase in the number of publications in this area in recent years.
An increased physical barrier produced by silicon deposition beneath leaf cuticles has long been considered to represent a major component underlying silicon-mediated plant resistance to insect pests. Silicon deposition contributes to increased rigidity and abrasiveness of plant tissues, thereby forming a mechanical barrier and reducing their palatability and digestibility to both vertebrate (Massey and Hartley, 2006, 2009) and invertebrate herbivores (Goussain et al., 2005; Kvedaras et al., 2007a; Massey and Hartley, 2009). Increased abrasiveness of leaves due to silicon deposition reduces food quality for herbivores and may cause wear of herbivore mouthparts, which further reduces feeding efficiency and growth rates (Massey and Hartley, 2009). Conversely, using a simple method to determine mandibular wear (Smith et al., 2007), it was shown that although there was a trend for increased wear in Eldana saccharina larvae that developed on silicon-treated sugarcane, the ability of larvae to renew their mandibles at each moult probably allows them to compensate for increased wear (Kvedaras et al., 2009). Finely ground wollastonite (CaSiO3) in artificial diets at rates of up to 3.3% silicon had no significant effect on larval growth of Helicoverpa armigera (Hübner; Lepidoptera: Noctuidae) and Helicoverpa punctigera Wallengren, suggesting that silicon may not be directly deleterious to insects via ingestion and other mechanisms may be involved in silicon-mediated plant resistance (Stanley et al., 2014). It should be noted, however, that by grinding the silicon, this has likely removed potential abrasive attributes, in addition to the potential effects of soluble-silicon-induced plant defenses.
Using energy-dispersive X-ray (EDX) and X-ray mapping, it was shown that the pattern of silicon deposition in sugarcane, especially at the internode and root band, is likely the reason (at least, in part) for enhanced resistance of silicon-treated sugarcane to penetration and feeding by E. saccharina at these sites (Keeping et al., 2009). Further, epidermal silicon was higher in the control (i.e., no silicon treatment), E. saccharina resistant cultivar, than the susceptible control cultivar, suggesting that such differences in silicon-mediated resistance exist to a large extent due to the varying ability of cultivars to deposit silicon within the stalk epidermis (Keeping et al., 2009), thus preventing E. saccharina penetration (Kvedaras and Keeping, 2007). A more recent study using scanning electron microscopy and EDX compared four grass species, and showed that spine and phytolith morphology both within and between species may be more important than leaf silicon concentration in determining the abrasiveness and/or digestibility of leaves and thus the effectiveness of anti-herbivore defense (Hartley et al., 2015). The authors showed that all the grasses tested were able to deposit new types of silicon-based structures when silicon supply was increased. These changes were particularly evident when the leaves were mechanically damaged; however, damage in the absence of additional silicon did not produce such structures (Hartley et al., 2015).
McNaughton and Tarrants (1983) were the first to show induction of silica. They showed that plants growing in a more heavily mammal-grazed grassland in the Serengeti, Tanzania, accumulated more silica in their leaf blades relative to plants from a less heavily grazed site, and blade silica content was higher when plants were defoliated, suggesting that silicification is an inducible defense against mammalian herbivores. Massey et al. (2007) demonstrated in a laboratory study, that feeding by both a mammal, M. agrestis and an insect, Schistocerca gregaria Forskal (Orthoptera: Acrididae) led to increased levels of silica in grass leaves. Other recent studies on arthropods have demonstrated that silicon-mediated anti-herbivore defense is both inducible and allelochemical-mediated (Gomes et al., 2005; Kvedaras et al., 2010; Costa et al., 2011) and these effects can complement the physical effects described above, leading to impaired feeding, growth, and development (Figure 1).
FIGURE 1. Summary of mechanisms by which application of silicon treatments to plants may affect the plant, herbivores and natural enemies. Ticks indicate empirically supported effects and question marks indicate untested effects. See text for details.
Increasing evidence shows that silicon treatment increases transcript levels of defense-related genes, thereby enhancing the activities of plant defensive enzymes (Liang et al., 2003; Cai et al., 2008; Rahman et al., 2015) leading to increased accumulation of defensive compounds, such as phenolics, phytoalexins, and momilactones (Fawe et al., 1998; Rodrigues et al., 2004; Rémus-Borel et al., 2005). Gomes et al. (2005) showed that the addition of silicon strongly enhanced wheat resistance to greenbug Schizaphis graminum (Rondani; Hemiptera: Aphididae). Further, silicon pre-treatment increased the activities of the defensive enzymes peroxidase, polyphenoloxidase, and phenylalanine ammonia lyase. In particular, silicon facilitated the strongest resistance if wheat plants had previously been infested with aphids. Chérif et al. (1994) found that silicon-treated cucumber plants show increased activity of the enzymes peroxidase, polyphenoloxidase, β-1,3 glucanase, and chitinase in response to infection by pathogens. Perennial ryegrass (Lolium perenne L.) grown in silicon-amended soil exhibited greater activity of peroxidase and polyphenoloxidase, higher levels of several phenolic acids, including chlorogenic acid and flavonoids, and enhanced expression levels of genes encoding phenylalanine ammonia lyase (PALa and PALb) and lipoxygenase (LOXa) in response to infection by Magnaporthe oryzae (T.T. Hebert) M.E. Barr (Rahman et al., 2015). Histological and ultrastructural analyses revealed that silicon mediates active localized cell defenses, and epidermal cells of silicon-treated plants displayed specific defense reactions including papilla formation, production of callose, and accumulation of glycosilated phenolics in response to pathogen infection by the fungus Blumeria graminis f. sp. tritici (DC.) Speer (Bélanger et al., 2003). Silicon-mediated brown spot resistance in rice plants is independent of the classic immune hormones, salicylic acid and jasmonic acid (JA; Van Bockhaven et al., 2015). Conversely, silicon mounted rice resistance to the brown spot fungus Cochliobolus miyabeanus (Ito and Kuribayashi) Dastur, by interfering with the production and/or action of fungal ethylene, prevents the fungus from suppressing the rice innate immune system (Van Bockhaven et al., 2015).
Pre-treatment with certain chemicals or previous biotic stressor may provoke a specific physiological state in plants called “priming” (Fauteux et al., 2006; Hao et al., 2012; Worrall et al., 2012; Aimé et al., 2013). Primed plants are thus physiologically prepared to induce quicker and/or stronger defense responses upon subsequent attack, providing plants with a more effective means to respond to challenges (Ton et al., 2006; Jung et al., 2009; Slaughter et al., 2012; Ye et al., 2013). A recent study demonstrates that silicon is able to prime jasmonate-mediated defense responses and rice defense against a chewing herbivore, the rice leaffolder, Cnaphalocrocis medinalis (Lepidoptera: Pyralidae; (Ye et al., 2013). More interestingly, activation of jasmonate signaling in turn promotes silicon accumulation in rice leaves, indicating a strong interaction between silicon and jasmonate in rice defense against insect herbivores. Some recent studies have shown that silicon can also prime plants for alleviating biotic stress imposed by pathogens (Ghareeb et al., 2011; Rahman et al., 2015). Vivancos et al. (2015) showed that priming is also an important mechanism of silicon-mediated resistance of Arabidopsis thaliana (L.) Heynh. against powdery mildew caused by Golovinomyces cichoracearum (DC.). Further, this work has also revealed that silicon may interfere with effector proteins released by such biotrophic pathogens, suggesting that mechanisms other than salicylic acid-dependent plant defense priming are involved (Vivancos et al., 2015). It has been suggested that priming of plant defense responses, alterations in phytohormone homeostasis, and interaction with defense signaling components are all potential mechanisms involved in regulating silicon-triggered resistance responses (Van Bockhaven et al., 2013). Silicon has also been demonstrated to prime plants for resistance against abiotic stresses (Ahmed et al., 2013). Research on silicon-mediated herbivore resistance lags far behind that on silicon-mediated disease resistance. Further studies are needed to determine the exact nature of silicon-primed anti-herbivore defense and indeed other mechanisms that may play a role in plant resistance to biotic stressors. For example, effectors that modulate plant defenses have also been identified in the saliva of insects (for a review see Hogenhout and Bos, 2011) and it is feasible that a similar mechanism proposed for plant pathogens, also operates for insects, although this remains to be elucidated.
Recent developments regarding the understanding of molecular mechanisms controlling silicon accumulation and the discovery of silicon transporters have enabled a ready ability to classify a plant as Si-competent, or not. This will enable a better understanding of the role of silicon in several fundamental aspects of ecology concerning plant fitness under stress (Deshmukh and Bélanger, 2015).
Natural enemies of herbivores can be important in the management of agricultural pest species. Evidence for this includes the wide literature on biological control using predators, parasitoids and entomopathogens. In this section we consider what is currently the least thoroughly investigated aspect of plant–silicon–herbivore interactions: the mechanisms by which the application of silicon compounds may affect the impact of natural enemies on herbivores.
Entomopathogens are increasingly used in arthropod pest management. However, as this approach uses applications of live organisms rather than chemicals, as in conventional insecticide use, particular attention needs to be given to maximizing the viability and impact of the treatment on the target pest. In work with the fungus Beauveria bassiana (Bals.-Criv.) Vuill., 1912, potassium silicate was added to nutrient solutions applied to plant roots seven days after inoculation with spider mite, Tetranychus urticae Koch (Gatarayiha et al., 2010). Potassium silicate alone did not kill the pest mites, but when used at the higher rates, equivalent to 80 and 160 mg of pure silicon per liter, pest mortality caused by B. bassiana was up to 92%. The authors of that study hypothesized that silicon application primed biochemical defenses in the plants (see above) which interfered with the feeding of mites making them more susceptible to the entomopathogen (Figure 1).
Of particular relevance to the possible effects of silicon on non-entomopathogenic natural enemies is a study of induced defense in rice (Ye et al., 2013). This study, employing rice mutant lines in which genes for jasmonate synthesis or jasmonate perception were silenced, showed a strong interaction between soil-applied silicon and JA in defense against insect herbivores. This involved priming of JA-mediated defense responses by silicon and the promotion of silicon accumulation by JA (Ye et al., 2013). While that work did not extend to considering natural enemies it is significant for third trophic level effects because it identified a relationship between silicon and JA. Silicon is translocated within plants in the form of monosilicic acid, Si(OH)4 which is reported as an elicitor for systemic stress signals including JA (Fauteux et al., 2005). JA, in turn, is the primary signaling pathway that is activated by chewing herbivores leading to herbivore-induced plant volatiles (HIPV) production (Dicke et al., 1999, 2009).
The first published study of the effects of silicon on plant defense in which HIPV-mediated effects has been the focus was in cucumber (Kvedaras et al., 2010). That work demonstrated that soil-applied silicon enhanced the attraction of the predator Dicranolaius bellulus (Guerin-Meneville; Coleoptera: Melyridae) to Helicoverpa armigera (Hubner; Lepidoptera: Noctuidae) infested cucumber plants in a Y-tube olfactometer bioassay. Further, a small-scale field trial, using H. armigera eggs affixed to potted cucumber plants, before they were placed in a field plot of lucerne, showed that increased biological control by “wild” predators was significantly higher for soil-applied, silicon-treated plants than for control plants (Kvedaras et al., 2010; Figure 2). The authors hypothesized that this was due to a change in the plant volatile profile (HIPVs) produced by cucumber plants when attacked by an herbivore. Additional studies to measure and identify the compounds produced by pest-infested silicon-treated and untreated cucumber plants are worthwhile. Similar work on grapevines has yielded preliminary evidence for volatile-mediated defenses to promote predator attraction to pest-infested plants (Connick, 2011). A study of the volatiles produced by grapevines infested by the Lepidoptera pest, grapevine moth Phalaenoides glycinae (Lewin; Lepidoptera: Noctuidae) found that soil applied potassium silicate had profound effects. Seven volatile compounds emitted from P. glycinae-infested grapevines were identified and n-heptadecane found to be produced in significant amounts only by silicon-treated plants. Cis-thio rose oxide production, in contrast, was significantly lower in silicon-treated grapevines. A second study in that thesis found that the attractiveness of grapevines infested with the lightbrown apple moth (Epiphyas postvittana (Walker; Lepidoptera: Tortricidae) was positively correlated with plant foliar tissue concentration of silicon (Connick, 2011).
FIGURE 2. The effect of prior treatment with potassium silicate (silicon+) and infestation with 10 H. armigera larvae/plant (pest+) on the proportion of prey eggs removed from potted cucumber plants over a 24-h period when exposed to predators in the field. (N = 4), columns with differing letters differ (LSD test, P = 0.05). (Reproduced with permission from Kvedaras et al., 2010).
The impact of natural enemies on herbivores may be enhanced by mechanisms other than induced, indirect defenses based on HIPVs. By extending development time, and particularly the period over which neonate larvae feed on the exterior of plants before being able to penetrate the plant cuticle and commence mining or boring, herbivores are exposed to a higher risk of attack by predators. Delayed penetration was evident in a study of sugarcane borer, E. saccharina (Kvedaras and Keeping, 2007). Massey and Hartley (2006) reported similar findings for Spodoptera exempta Walker feeding on grass with high silicon levels. Many natural enemies forage for prey by locomotion over the foliar surface, so the practice of applying silicon treatments to the above ground plant parts could have physical or chemical effects on natural enemy foraging (Figure 1). Examples of recent studies that included treatments with foliar applied silicon are Dalastra et al. (2011) and de Assis et al. (2012, 2013), and in the latter of those studies, there was no effect of foliar treatments to potato plants on predatory beetles, although the plants treated with silicic acid were less preferred by defoliators. Further work needs to test for the strength of such effects on a wider range of natural enemy taxa.
Foraging of predators may also be affected by foliar pubescence, especially glandular trichomes. The latter produce irritant, toxic and adhesive liquid secretions from the tips that can provide high levels of protection from foliar-associated herbivores (Gurr and McGrath, 2002) but can also affect natural enemies (Simmons and Gurr, 2004, 2005). When subject to herbivores, plants have the capacity to regenerate new leaves that exhibit enhanced densities of trichomes, an induced defense that is under the control of JA (Yoshida et al., 2009). This form of induced defense is remarkable in taking place over days rather than the timespan of hours as in the case of induced production of semiochemical volatiles. This phenomenon has relevance to the interplay between silicon and plant defense because plant-available silicon influences the JA signaling pathway (Ye et al., 2013). Accordingly, the phenomenon of herbivore-attacked plants producing more hirsute foliage is another form of plant defense that we hypothesize may by amplified by silicon pre-treatment (Figure 1).
Not only might plant-available silicon promote the density of trichomes on young foliage, work on deposition patterns of silica in the leaf epidermis suggests that the bases of trichomes is a major site in cucumber (Samuels et al., 1991a,b), while in the grasses D. cespitosa and Festuca ovina L., silica was particularly evident in the tips of spines under control conditions, but was distributed throughout the spine and the leaf surface when silicon fertilized (Hartley et al., 2015). The epicarp hairs present on the mature caryopses of the four cereals, barley, oats, rye, and wheat (Bennett and Parry, 1981) are also important silicon deposition sites, particularly in the tips of hairs where it is most likely to promote adverse effects on herbivores including – potentially – human consumers of grain products (Parry et al., 1984). It remains to be tested whether the potentially adverse effects of trichomes on predators are exacerbated by silicon supplementation and the extent to which any such effects are offset by stronger effects on herbivores.
Among studies of the effects of silicon on pests that do consider third trophic level effects, these tend to use designs that are not well suited to detecting the full range of possible mechanisms that may operate. An example is work by Moraes et al. (2004), with the lacewing Chrysoperla externa Steinmann in which wheat aphid (Schizaphis graminum (Rondani; Hemiptera: Aphididae) prey were removed from the test plants before being exposed to the predators. Since predators were not exposed to plants or their volatiles, they would have been unable to detect HIPV-mediated effects, though effects related to prey quality could be assessed.
A major limit on our current understanding of the effects of silicon on natural enemies is the apparent absence of studies on below-ground effects. Many arthropod pests cause important damage to plant roots so studies of how silicon might promote natural enemies such as predacious beetle larvae and entomopathogenic nematodes would be valuable.
Of the three types of natural enemies, parasitoids are the least well studied in relation to plant available silicon, though many of the comments made above, for established and possible effects on predators (Figure 1), will apply to parasitoids. Of particular significance is the wealth of evidence for HIPVs attracting parasitoids to pest-infested plants (Dicke et al., 2009). The only study with silicon-treated and un-treated plants in which a parasitoid was considered is that by Moraes et al. (2004) with Aphidius colemani Viereck (Hymenoptera: Aphididae). Unfortunately, this confined wasps to narrowly spaced wheat plants and, because it used non-choice conditions, would not have allowed HIPV-mediated effects to be apparent.
How “Omics” Support Plant Defense Studies?
To understand how the addition of silicon to a plant’s environment can improve plant defense, the plant as a whole must be considered through global analysis of the major responsive components of the DNA, RNA, proteins, and metabolites which are then holistically viewed using bioinformatics (Figure 3).
FIGURE 3. The workflow for the application of -omics technology to quantify phenotypic changes in plants due to silicon treatment. In a comparative study, parallel samples are grown under laboratory conditions or in the field with one subject to silicon treatment. After the application of appropriate sample extraction techniques to obtain mRNA, proteins or metabolites in an unbiased and comprehensive manner, the samples are subjected to parallel analysis to obtain a comprehensive dataset of the transcriptome, proteome, and metabolome. These datasets are then analyzed in bioinformatic pipelines to identify the components and quantify the differences in abundance of specific mRNAs, proteins or metabolites, which can then be related to phenotypic changes in the plant, such as resistance to a herbivore or pathogen. This information can then be utilized in crop management practices. A similar analysis could be applied to an ecological system, in order to understand the role of silicon (whether naturally occurring or supplemented) in ecological processes, for example comparing grazed versus ungrazed pastures.
While system-wide analysis has long been applied to plants, their application to analyzing plant defense has been limited (Chen et al., 2005; Giri et al., 2006; Thivierge et al., 2010; Lewandowska-Gnatowska et al., 2011; Duceppe et al., 2012; Timbo et al., 2014) and analyzing silicon’s role even more so. Numerous reductionist experiments targeting specific proteins or enzymes have shown that silicon treatment induces plant defensive enzymes (Liang et al., 2003; Cai et al., 2008), leading to the accumulation of defensive compounds and metabolites (Fawe et al., 1998; Rodrigues et al., 2004).
But the power of -omics approaches lies in its non-targeted nature, allowing the unearthing of unexpected changes. Transcriptome analysis represents the only -omic analysis of silicon’s effects, with a study on challenged A. thaliana showing silicon treatment causes a decrease in primary metabolism that allows a more efficient defense response (Fauteux et al., 2006). A similar analysis was also conducted on rice (Ye et al., 2013), as indicated above. Recent work has sought to establish the “Prime-ome”, or the mechanism behind how a plant defends itself or is in a “primed state” to rapidly respond to attack by insects and microbial pathogens (Balmer et al., 2015). Not surprisingly, the available -omics scale data shows that the plant’s response depends on the priming inducer and the pathogen, which is also observed in defense against arthropods (Balmer et al., 2015). Silicon’s role in defense against herbivores remains vastly understudied by -omics methodologies which would reveal the role of, as yet, untargeted molecules, including proteins and metabolites, through global analysis.
Transcriptomics alone is insufficient to understand an organism’s phenotype (Barah and Bones, 2015) as it is the proteome and metabolome that provide the molecular mechanisms that allow a plant to defend itself (Oliveira et al., 2014). While proteomics and metabolomics are rapidly maturing fields, they are still limited by the issues of throughput and the depth of proteome and metabolome coverage due to the dynamic range of concentration of the molecules present (Jorge et al., 2015). The abundance of proteins can vary by 7–10 orders of magnitude (Ly and Wasinger, 2008; Zubarev, 2013) and the existence of a proteoform is often reported by the detection of only a single peptide (Mallick et al., 2007). Without an equivalent of PCR utilized in genomics and transcriptomics, the only way to reliably detect and quantify the abundance of low copy number proteins is to start with more material (Zubarev, 2013) and fractionate the proteins to isolate those of high abundance from the rest (Stasyk and Huber, 2004; Righetti et al., 2005; Ly and Wasinger, 2011). The same logic applies to metabolites but in both cases the number of fractions requiring analysis increases.
In the case of proteomics, fractionation of intact proteins reduces this increase compared to “shotgun” peptide-centric methods while retaining the option of utilizing 2D-PAGE as a further fractionation and quantification method (Coorssen and Yergey, 2015). To determine plant defense responses as a result of silicon treatment, 2D-PAGE has the distinct advantage of quantifying protein abundance changes prior to identification. This is contrary to LC/MS/MS methodologies where identification of peptides and their assignment to a protein isoform needs to be performed prior to quantitation. Thus, 2D-PAGE can decrease the number of samples requiring analyses by mass spectrometry (MS), freeing valuable instrument time. In proteomics, the issue of throughput is being addressed somewhat by faster instrument scan speeds (Richards et al., 2015), the adoption of ultra high-pressure chromatography (Kocher et al., 2011; Thakur et al., 2011) and data-independent acquisition (DIA) techniques in LC/MS/MS (Huang et al., 2015). DIA methodologies have also been applied to measure nitrogen flux and metabolism (Ullmann-Zeunert et al., 2012) indicating that DIA could have application in quantitative metabolomics, in order to assess how changes in the levels of specific metabolites can be related to observed plant defensive phenotypes.
Conclusions and Future Directions
There is now considerable literature supporting the role of silicon as a physical defense mechanism, and a growing number of published works on the role of silicon-mediated biochemical defense. However, there are few references on the role of silicon in tri-trophic interactions.
Research should focus on understanding the relative importance of both physical and biochemical defence and how (if) this differs between herbivores. A meta-analysis of the literature would be valuable to discern if silicon has a greater effect in certain feeding guilds or taxons. Understanding the interaction between silicon and the plant defense pathways, and if there is a similar mechanism acting against insects, and pathogens, will also be paramount, as there is a wealth of literature on silicon/pathogen interactions that can inform arthropod work.
Future researchers need to address the lack of knowledge on below-ground effects of silicon application to plants on predators. There is a more general dearth of knowledge on how silicon might alter root toughness and chemical defenses. There is also a need to test for the effects of foliar deposits from foliar applied silicon on natural enemy foraging and impact. Work also needs to consider the possibility that changing the plant surface, by denser or more robust trichomes, may have negative effects on natural enemy foraging (Figure 1). More generally, workers need to consider the effects of silicon under field conditions (something done quite extensively for mammals in natural ecological systems) and be less reliant on greenhouse and laboratory studies, especially those that make it impossible for natural enemy mediated effects on herbivores to be apparent. Finally, there are currently no published studies of the effects of silicon on HIPV production but such work is known to be underway. If strong evidence is forthcoming for effects on the blend of HIPVs, this will add impetus to the need for greater attention to be given to the third trophic level in studies of silicon on plant defenses.
Using system-wide analysis or -omics technologies would permit us to not only understand silicon’s role in the production of defense-related compounds, but in the production of HIPVs, in addition to the associated energy costs to the plant. This could potentially inform the manipulation of plants to minimize herbivory and maximize the impact of natural enemies.
Modern approaches of transcriptomics, proteomics, metabolomics, and transgenic mutants will serve as powerful tools for dissecting the underlying mechanism/s involved in silicon and plant defense. In an era when sustainable pest management is receiving more attention than ever before, due largely to restrictions or the withdrawal of toxic pesticides, because of their negative impacts on human and environmental health, silicon treatment should be more widely considered and tested as a pest management option.
OR and GG developed the concept, drafted, and critically revised the manuscript. MP and RZ drafted and critically revised the manuscript.
GG is supported by the National Thousand Talents Program in China and the Advanced Talents of SAEFA, and OR is supported by the Jinshan Scholar Program at Fujian Agriculture and Forestry University, Fujian Province, China.
Conflict of Interest Statement
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
The reviewer JD and handling Editor declared their shared affiliation, and the handling Editor states that the process nevertheless met the standards of a fair and objective review.
The authors thank Mrs. Anne Johnson for assistance with manuscript production and two reviewers, who made useful comments on the manuscript.
Ahmed, M., Kamran, A., Asif, M., Qadeer, U., Ahmed, Z. I., and Goyal, A. (2013). Silicon priming: a potential source to impart abiotic stress tolerance in wheat: a review. Aust. J. Crop Sci. 7, 484–491.
Aimé, S., Alabouvette, C., Steinberg, C., and Olivain, C. (2013). The endophytic strain Fusarium oxysporum Fo47: a good candidate for priming the defense responses in tomato roots. Mol. Plant Microbe Interact. 26, 918–926. doi: 10.1094/MPMI-12-12-0290-R
Alexandre, A., Meunier, J. D., Colin, F., and Koud, J. M. (1997). Plant impact on the biogeochemical cycle of silicon and related weathering processes. Geochim. Cosmochim. Acta 61, 677–682. doi: 10.1016/s0016-7037(97)00001-x
Anda, M., Suryani, E., Husnain, and Subardja, D. (2015). Strategy to reduce fertilizer application in volcanic paddy soils: nutrient reserves approach from parent materials. Soil Tillage Res. 150, 10–20. doi: 10.1016/j.still.2015.01.005
Barah, P., and Bones, A. M. (2015). Multidimensional approaches for studying plant defence against insects: from ecology to omics and synthetic biology. J. Exp. Bot. 66, 479–493. doi: 10.1093/jxb/eru489
Bélanger, R., Benhamou, N., and Menzies, J. (2003). Cytological evidence of an active role of silicon in wheat resistance to powdery mildew (Blumeria graminis f. sp. tritici). Phytopathology 93, 402–412. doi: 10.1094/PHYTO.2003.93.4.402
Bennett, D. M., and Parry, D. W. (1981). Electron-probe microanalysis studies of silicon in the epicarp hairs of the caryopses of Hordeum sativum Jess., Avena sativa L., Secale cereale L. and Triticum aestivum L. Ann. Bot. 48, 645–654.
Cai, K., Gao, D., Luo, S., Zeng, R., Yang, J., and Zhu, X. (2008). Physiological and cytological mechanisms of silicon-induced resistance in rice against blast disease. Physiol. Plant. 134, 324–333. doi: 10.1111/j.1399-3054.2008.01140.x
Chain, F., Cote-Beaulieu, C., Belzile, F., Menzies, J. G., and Belanger, R. R. (2009). A comprehensive transcriptomic analysis of the effect of silicon on wheat plants under control and pathogen stress conditions. Mol. Plant Microbe Interact. 22, 1323–1330. doi: 10.1094/MPMI-22-11-1323
Chen, H., Wilkerson, C. G., Kuchar, J. A., Phinney, B. S., and Howe, G. A. (2005). Jasmonate-inducible plant enzymes degrade essential amino acids in the herbivore midgut. Proc. Natl. Acad. Sci. U.S.A. 102, 19237–19242. doi: 10.1073/pnas.0509026102
Connick, V. J. (2011). The Impact of Silicon Fertilisation on the Chemical Ecology of Grapevine, Vitis vinifera Constitutive and Induced Chemical Defences Against Arthropod Pests and Their Natural Enemies. Ph.D. thesis, Charles Sturt University, Albury–Wodonga, NSW.
Cornelis, J. T., Delvaux, B., Georg, R. B., Lucas, Y., Ranger, J., and Opfergelt, S. (2011). Tracing the origin of dissolved silicon transferred from various soil-plant systems towards rivers: a review. Biogeosciences 8, 89–112. doi: 10.5194/bg-8-89-2011
Correa, R. S. B., Moraes, J. C., Auad, A. M., and Carvalho, G. A. (2005). Silicon and acibenzolar-s-methyl as resistance inducers in cucumber, against the whitefly Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) Biotype B. Neotrop. Entomol. 34, 429–433. doi: 10.1590/S1519-566X2005000300011
Costa, R. R., Moraes, J. C., and DaCosta, R. R. (2011). Feeding behaviour of the greenbug Schizaphis graminum on wheat plants treated with imidacloprid and/or silicon. J. Appl. Entomol. 135, 115–120. doi: 10.1111/j.1439-0418.2010.01526.x
Dalastra, C., Campos, A. R., Fernandes, F. M., Martins, G. L. M., and Campos, Z. R. (2011). Silicon as a resistance inducer controlling the silvering thrips Enneothrips flavens Moulton, 1941 (Thysanoptera: Thripidae) and its effects on peanut yield. Ciênc. Agrotecnol. 35, 531–538. doi: 10.1590/S1413-70542011000300014
Dallagnol, L. J., Rodrigues, F. A., Pascholati, S. F., Fortunato, A. A., and Camargo, L. E. A. (2015). Comparison of root and foliar applications of potassium silicate in otentiating post-infection defences of melon against powdery mildew. Plant Pathol. 64, 1085–1093. doi: 10.1111/ppa.12346
de Assis, F., Moraes, J. C., Silveira, L., Francoso, J., Nascimento, A. M., and Antunes, C. (2012). Inducers of resistances in potato and its effects on defoliators and predatory insects. Rev. Colomb. Entomol. 38, 30–34.
de Assis, F. A., Moraes, J. C., Auad, A. M., and Coelho, M. (2013). The effects of foliar spray application of silicon on plant damage levels and components of larval biology of the pest butterfly Chlosyne lacinia saundersii (Nymphalidae). Int. J. Pest Manag. 59, 128–134. doi: 10.1080/09670874.2013.779049
Deren, C. W. (2001). “Plant genotype, silicon concentration, and silicon-related repsonses,” in Silicon in Agriculture, eds L. E. Datnoff, G. H. Snyder, and G. H. Korndorfer (Amsterdam: Elsevier Science), 149–158.
Deshmukh, R. K., Vivancos, J., Guerin, V., Sonah, H., Labbe, C., Belzile, F., et al. (2013). Identification and functional characterization of silicon transporters in soybean using comparative genomics of major intrinsic proteins in Arabidopsis and rice. Plant Mol. Biol. 83, 303–315. doi: 10.1007/s11103-013-0087-3
Dicke, M., Gols, R., Ludeking, D., and Posthumus, M. A. (1999). Jasmonic acid and herbivory differentially induce carnivore-attracting plant volatiles in lima bean plants. J. Chem. Ecol. 25, 1907–1922. doi: 10.1023/A:1020942102181
Duceppe, M. O., Cloutier, C., and Michaud, D. (2012). Wounding, insect chewing and phloem sap feeding differentially alter the leaf proteome of potato, Solanum tuberosum L. Proteome Sci. 10, 73. doi: 10.1186/1477-5956-10-73
Fauteux, F., Chain, F., Belzile, F., Menzies, J. G., and Belanger, R. R. (2006). The protective role of silicon in the Arabidopsis-powdery mildew pathosystem. Proc. Natl. Acad. Sci. U.S.A. 103, 17554–17559. doi: 10.1073/pnas.0606330103
Fauteux, F., Rémus-Borel, W., Menzies, J. G., and Bélanger, R. R. (2005). Silicon and plant disease resistance against pathogenic fungi. FEMS Microbiol. Lett. 249, 1–6. doi: 10.1016/j.femsle.2005.06.034
Gatarayiha, M. C., Laing, M. D., and Miller, R. M. (2010). Combining applications of potassium silicate and Beauveria bassiana to four crops to control two spotted spider mite, Tetranychus urticae Koch. Int. J. Pest Manag. 56, 291–297. doi: 10.1080/09670874.2010.495794
Ghareeb, H., Bozsó, Z., Ott, P. G., Repenning, C., Stahl, F., and Wydra, K. (2011). Transcriptome of silicon-induced resistance against Ralstonia solanacearum in the silicon non-accumulator tomato implicates priming effect. Physiol. Mol. Plant Pathol. 75, 83–89. doi: 10.1016/j.pmpp.2010.11.004
Giri, A. P., Wunsche, H., Mitra, S., Zavala, J. A., Muck, A., Svatos, A., et al. (2006). Molecular interactions between the specialist herbivore Manduca sexta (Lepidoptera, Sphingidae) and its natural host Nicotiana attenuata. VII. Changes in the plant’s proteome. Plant Physiol. 142, 1621–1641. doi: 10.1104/pp.106.088781
Goussain, M. M., Prado, E., and Moraes, J. C. (2005). Effect of silicon applied to wheat plants on the biology and probing behaviour of the greenbug Schizaphis graminum (Rond.) (Hemiptera: Aphididae). Neotrop. Entomol. 34, 807–813. doi: 10.1590/S1519-566X2005000500013
Gŕegoire, C., Remus-Borel, W., Vivancos, J., Labbe, C., Belzile, F., and Bélanger, R. R. (2012). Discovery of a multigene family of aquaporin silicon transporters in the primitive plant Equisetum arvense. Plant J. 72, 320–330. doi: 10.1111/j.1365-313X.2012.05082.x
Guével, M. H., Menzies, J. G., and Bélanger, R. R. (2007). Effect of root and foliar applications of soluble silicon on powdery mildew control and growth of wheat plants. Euro. J. Plant Pathol. 119, 429–436. doi: 10.1007/s10658-007-9181-1
Gurr, G. M., and Kvedaras, O. L. (2010). Synergizing biological control: scope for sterile insect technique, induced plant defences and cultural techniques to enhance natural enemy impact. Biol. Control 52, 198–207. doi: 10.1016/j.biocontrol.2009.02.013
Han, W. X., Fang, J. Y., Reich, P. B., Woodward, F. I., and Wang, Z. H. (2011). Biogeography and variability of eleven mineral elements in plant leaves across gradients of climate, soil and plant functional type in China. Ecol. Lett. 14, 788–796. doi: 10.1111/j.1461-0248.2011.01641.x
Han, Y., Lei, W., Wen, L., and Hou, M. (2015). Silicon-mediated resistance in a susceptible rice variety to the rice leaf folder, Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae). PLoS ONE 10:0120557. doi: 10.1371/journal.pone.0120557
Hao, Z., Fayolle, L., van Tuinen, D., Chatagnier, O., Li, X., Gianinazzi, S., et al. (2012). Local and systemic mycorrhiza-induced protection against the ectoparasitic nematode Xiphinema index involves priming of defence gene responses in grapevine. J. Exp. Bot. 63, 3657–3672.
Hartley, S. E., Fitt, R. N., McLarnon, E. L., and Wade, R. N. (2015). Defending the leaf surface: intra- and inter-specific differences in silicon deposition in grasses in response to damage and silicon supply. Front. Plant Sci. 6:35. doi: 10.3389/fpls.2015.00035
He, W., Yang, M., Li, Z., Qiu, J., Liu, F., Qu, X., et al. (2015). High levels of silicon provided as a nutrient in hydroponic culture enhances rice plant resistance to brown planthopper. Crop Protect. 67, 20–25. doi: 10.1016/j.cropro.2014.09.013
Henriet, C., Draye, X., Oppitz, I., Swennen, R., and Delvaux, B. (2006). Effects, distribution and uptake of silicon in banana (Musa spp.) under controlled conditions. Plant Soil 287, 359–374. doi: 10.1007/s11104-006-9085-4
Hou, M., and Han, Y. (2010). Silicon-mediated rice plant resistance to the asiatic rice borer (lepidoptera: crambidae): effects of silicon amendment and rice varietal resistance. J. Econ. Entomol. 103, 1412–1419. doi: 10.1603/ec09341
Huitu, O., Forbes, K. M., Helander, M., Julkunen-Tiitto, R., Lambin, X., Saikkonen, K., et al. (2014). Silicon, endophytes and secondary metabolites as grass defenses against mammalian herbivores. Front. Plant Sci. 5:478. doi: 10.3389/fpls.2014.00478
International Plant Nutrition Institute [IPNI] (2015). Nutri-Facts. Silicon. No. 14. Available at: http://www.ipni.net/publication/nutrifacts-na.nsf/0/A7B4AB4D35C153BF85257ECE006E0E34/$FILE/NutriFacts-NA-14.pdf [accessed April 4, 2016].
Jorge, T. F., Rodrigues, J. A., Caldana, C., Schmidt, R., van Dongen, J. T., Thomas-Oates, J., et al. (2015). Mass spectrometry-based plant metabolomics: metabolite responses to abiotic stress. Mass Spectrom. Rev. doi: 10.1002/mas.21449 [Epub ahead of print].
Keeping, M. G., and Kvedaras, O. L. (2008). Silicon as a plant defence against insect herbivory: response to massey, ennos and hartley. J. Anim. Ecol. 77, 631–633. doi: 10.1111/j.1365-2656.2008.01380.x
Keeping, M. G., Kvedaras, O. L., and Bruton, A. G. (2009). Epidermal silicon in sugarcane: cultivar differences and role in resistance to sugarcane borer Eldana saccharina. Environ. Exp. Bot. 66, 54–60. doi: 10.1016/j.envexpbot.2008.12.012
Keeping, M. G., Meyer, J. H., and Sewpersad, C. (2013). Soil silicon amendments increase resistance of sugarcane to stalk borer Eldana saccharina walker (Lepidoptera: Pyralidae) under field conditions. Plant Soil 363, 297–318. doi: 10.1007/s11104-012-1325-1
Kocher, T., Swart, R., and Mechtler, K. (2011). Ultra-high-pressure RPLC hyphenated to an LTQ-Orbitrap velos reveals a linear relation between peak capacity and number of identified peptides. Anal. Chem. 83, 2699–2704. doi: 10.1021/ac103243t
Korndorfer, A. P., Cherry, R., and Nagata, R. (2004). Effect of calcium silicate on feeding and development of tropical sod webworms (Lepidoptera: Pyralidae). Flor. Entomol. 87, 393–395. doi: 10.1653/0015-4040(2004)087[0393:EOCSOF]2.0.CO;2
Kvedaras, O. L., An, M., Choi, Y. S., and Gurr, G. M. (2010). Silicon enhances natural enemy attraction and biological control through induced plant defences. Bull. Entomol. Res. 100, 367–371. doi: 10.1017/S0007485309990265
Kvedaras, O. L., Byrne, M. J., Coombes, N. E., and Keeping, M. G. (2009). Influence of plant silicon and sugarcane cultivar on mandibular wear in the stalk borer Eldana saccharina. Agric. For. Entomol. 11, 301–306. doi: 10.1111/j.1461-9563.2009.00430.x
Kvedaras, O. L., Keeping, M. G., Goebel, F. R., and Byrne, M. J. (2007a). Larval performance of the pyralid borere Eldana saccharina walker and stalk damage in sugarcane: influence of plant silicon, cultivar and feeding site. Int. J. Pest Manag. 53, 183–195. doi: 10.1080/09670870601110956
Kvedaras, O. L., Keeping, M. G., Goebel, R., and Byrne, M. (2007b). Water stress augments silicon-mediated resistance of susceptible sugarcane cultivars to the stalk borer, Eldana saccharina (Lepidoptera: Pyralidae). Bull. Entomol. Res. 97, 175–183. doi: 10.1017/S0007485307004853
Lewandowska-Gnatowska, E., Johnston, M. L., Antoine, W., Szczegielniak, J., Muszynska, G., and Miernyk, J. A. (2011). Using multiplex-staining to study changes in the maize leaf phosphoproteome in response to mechanical wounding. Phytochemistry 72, 1285–1292. doi: 10.1016/j.phytochem.2011.01.030
Liang, Y., Chen, Q., Liu, Q., Zhang, W., and Ding, R. (2003). Exogenous silicon (Si) increases antioxidant enzyme activity and reduces lipid peroxidation in roots of salt-stressed barley (Hordeum vulgare L.). J. Plant Physiol. 160, 1157–1164. doi: 10.1078/0176-1617-01065
Liang, Y., Hua, H., Zhu, Y.-G., Zhang, J., Cheng, C., and Roemheld, V. (2006). Importance of plant species and external silicon concentration to active silicon uptake and transport. New Phytol. 172, 63–72. doi: 10.1111/j.1469-8137.2006.01797.x
Liang, Y. C., Ma, T. S., Li, F. J., and Feng, Y. J. (1994). Silicon availability and response of rice and wheat to silicon in calcareous soils. Commun. Soil Sci. Plant Anal. 25, 2285–2297. doi: 10.1080/00103629409369189
Liang, Y. C., Sun, W. C., Si, J., and Römheld, V. (2005). Effects of foliar- and root-applied silicon on the enhancement of induced resistance to powdery mildew in Cucumis sativus. Plant Pathol. 54, 678–685. doi: 10.1111/j.1365-3059.2005.01246.x
Mallick, P., Schirle, M., Chen, S. S., Flory, M. R., Lee, H., Martin, D., et al. (2007). Computational prediction of proteotypic peptides for quantitative proteomics. Nat. Biotechnol. 25, 125–131. doi: 10.1038/nbt1275
Massey, F. P., and Hartley, S. E. (2006). “Experimental demonstration of the antiherbivore effects of silica in grasses: impacts on foliage digestibility and vole growth rates,” in Proceedings of the Royal Society of London Royal Society, London, 2299–2304.
Massey, F. P., and Hartley, S. E. (2009). Physical defences wear you down: progressive and irreversible impacts of silica on insect herbivores. J. Anim. Ecol. 78, 281–291. doi: 10.1111/j.1365-2656.2008.01472.x
Massey, F. P., Massey, K., Ennos, A. R., and Hartley, S. E. (2009). Impacts of silica-based defences in grasses on the feeding preferences of sheep. Basic Appl. Ecol. 10, 622–630. doi: 10.1016/j.baae.2009.04.004
McNaughton, S. J., and Tarrants, J. L. (1983). Grass leaf silicification: natural selection for an inducible defense against herbivores. Proc. Natl. Acad. Sci. U.S.A. 80, 790–791. doi: 10.1073/pnas.80.3.790
Miles, N., Manson, A. D., Rhodes, R., van Antwerpen, R., and Weigel, A. (2014). Extractable silicon in soils of the South African sugar industry and relationships with crop uptake. Commun. Soil Sci. Plant Anal. 45, 2949–2958. doi: 10.1080/00103624.2014.956881
Mitani, N., Chiba, Y., Yamaji, N., and Ma, J. F. (2009a). Identification and characterization of maize and barley Lsi2-Like silicon efflux transporters reveals a distinct silicon uptake system from that in rice. Plant Cell 21, 2133–2142. doi: 10.1105/tpc.109.067884
Mitani, N., Yamaji, N., Ago, Y., Iwasaki, K., and Ma, J. F. (2011). Isolation and functional characterization of an influx silicon transporter in two pumpkin cultivars contrasting in silicon accumulation. Plant J. 66, 231–240. doi: 10.1111/j.1365-313X.2011.04483.x
Mitani-Ueno, N., Yamaji, N., and Ma, J. F. (2011). Silicon efflux transporters isolated from two pumpkin cultivars contrasting in Si uptake. Plant Signal. Behav. 6, 991–994. doi: 10.4161/psb.6.7.15462
Montpetit, J., Vivancos, J., Mitani-Ueno, N., Yamaji, N., Remus-Borel, W., Belzile, F., et al. (2012). Cloning, functional characterization and heterologous expression of TaLsi1, a wheat silicon transporter gene. Plant Mol. Biol. 79, 35–46. doi: 10.1007/s11103-012-9892-3
Moraes, J. C., Goussain, M. M., Basagli, M. A. B., Carvalho, G. A., Ecole, C. C., and Sampaio, M. V. (2004). Silicon influence on the tritrophic interaction: wheat plants, the greenbug Schizaphis graminum (Rondani) (Hemiptera: Aphididae), and its natural enemies, Chrysoperla externa (Hagen) (Neuroptera: Chrysopidae) and Aphidius colemani viereck (Hymenoptera: Aphidiidae). Neotrop. Entomol. 33, 619–624.
Parry, D. W., Hodson, M. J., Sangster, A. G., Jones, W. C., and Neill, C. H. (1984). Some recent advances in studies of silicon in higher plants [and discussion]. Philos. Trans. R. Soc. Lond. B Biol. Sci. 304, 537–549. doi: 10.1098/rstb.1984.0045
Rahman, A., Wallis, C., and Uddin, W. (2015). Silicon induced systemic defense responses in perennial ryegrass against infection by Magnaporthe oryzae. Phytopathology 105, 748–757. doi: 10.1094/PHYTO-12-14-0378-R
Rémus-Borel, W., Menzies, J. G., and Bélanger, R. R. (2005). Silicon induces antifungal compounds in powdery mildew-infected wheat. Physiol. Mol. Plant Pathol. 66, 108–115. doi: 10.1016/j.pmpp.2005.05.006
Reynolds, J. J. H., Lambin, X., Massey, F. P., Reidinger, S., Sherratt, J. A., Smith, M. J., et al. (2012). Delayed induced silica defences in grasses and their potential for destabilising herbivore population dynamics. Oecologia 170, 445–456. doi: 10.1007/s00442-012-2326-8
Richards, A. L., Hebert, A. S., Ulbrich, A., Bailey, D. J., Coughlin, E. E., Westphall, M. S., et al. (2015). One-hour proteome analysis in yeast. Nat. Protoc. 10, 701–714. doi: 10.1038/nprot.2015.040
Righetti, P. G., Castagna, A., Antonioli, P., and Boschetti, E. (2005). Prefractionation techniques in proteome analysis: the mining tools of the third millennium. Electrophoresis 26, 297–319. doi: 10.1002/elps.200406189
Rodrigues, F. A., McNally, D. J., Datnoff, L. E., Jones, J. B., Labbe, C., Benhamou, N., et al. (2004). Silicon enhances the accumulation of diterpenoid phytoalexins in rice: a potential mechanism for blast resistance. Phytopathology 94, 177–183. doi: 10.1094/PHYTO.2004.94.2.177
Samuels, A. L., Glass, A. D. M., Ehret, D. L., and Menzies, J. G. (1991a). Distribution of silicon in cucumber leaves during infection by powdery mildew fungus (Sphaerotheca fuliginea). Can. J. Bot. 69, 140–146. doi: 10.1139/b91-020
Samuels, A. L., Glass, A. D. M., Ehret, D. L., and Menzies, J. G. (1991b). Mobility and deposition of silicon in cucumber plants. Plant Cell Environ. 14, 485–492. doi: 10.1111/j.1365-3040.1991.tb01518.x
Silva, R. V., de Lima Oliveira, R. D. A., da Silva Ferreira, P., Castro, D. B., and Rodrigues, F. A. (2015). Effects of silicon on the penetration and reproduction events of Meloidogyne exigua on coffee roots. Bragantia 74, 196–199. doi: 10.1590/1678-4499.360
Simmons, A. T., and Gurr, G. M. (2004). Trichome-based host plant resistance of Lycopersicon species and the biocontrol agent Mallada signata: are they compatible? Entomol. Exp. Appl. 113, 95–101. doi: 10.1111/j.0013-8703.2004.00210.x
Simmons, A. T., and Gurr, G. M. (2005). Trichomes of Lycopersicon species and their hybrids: effects on pests and natural enemies. Agric. For. Entomol. 7, 265–276. doi: 10.1111/j.1461-9555.2005.00271.x
Slaughter, A., Daniel, X., Flors, V., Luna, E., Hohn, B., and Mauch-Mani, B. (2012). Descendants of primed Arabidopsis plants exhibit resistance to biotic stress. Plant Physiol. 158, 835–843. doi: 10.1104/pp.111.191593
Smith, M. T., Kvedaras, O. L., and Keeping, M. G. (2007). A novel method to determine larval mandibular wear of the African stalk borer, Eldana saccharina walker (Lepidoptera: Pyralidae). Afr. Entomol. 15, 204–208. doi: 10.4001/1021-3589-15.1.204
Stanley, J. N., Baqir, H. A., and McLaren, T. I. (2014). Effect on larval growth of adding finely ground silicon-bearing minerals (wollastonite or olivine) to artificial diets for Helicoverpa spp. (Lepidoptera: Noctuidae). Austral Entomol. 53, 436–443. doi: 10.1111/aen.12086
Struyf, E., Smis, A., Van Damme, S., Garnier, J., Govers, G., Van Wesemael, B., et al. (2010). Historical land use change has lowered terrestrial silica mobilization. Nat. Commun. 1:129. doi: 10.1038/ncomms1128
Thakur, S. S., Geiger, T., Chatterjee, B., Bandilla, P., Frohlich, F., Cox, J., et al. (2011). Deep and highly sensitive proteome coverage by LC-MS/MS without prefractionation. Mol. Cell. Proteom. 10:M110003699. doi: 10.1074/mcp.M110.003699
Thivierge, K., Prado, A., Driscoll, B. T., Bonneil, E., Thibault, P., and Bede, J. C. (2010). Caterpillar- and salivary-specific modification of plant proteins. J. Proteome Res. 9, 5887–5895. doi: 10.1021/pr100643m
Timbo, R. V., Hermes-Lima, M., Silva, L. P., Mehta, A., Moraes, M. C., and Paula, D. P. (2014). Biochemical aspects of the soybean response to herbivory injury by the brown stink bug Euschistus heros (Hemiptera: Pentatomidae). PLoS ONE 9:e109735. doi: 10.1371/journal.pone.0109735
Ton, J., Pieterse, C. M., and Van Loon, L. (2006). “The relationship between basal and induced resistance in Arabidopsis,” in Multigenic and Induced Systemic Resistance in Plants, eds S. Tuzun and E. Bent (New York, NY: Springer), 197–224.
Ullmann-Zeunert, L., Muck, A., Wielsch, N., Hufsky, F., Stanton, M. A., Bartram, S., et al. (2012). Determination of (1)(5)N-incorporation into plant proteins and their absolute quantitation: a new tool to study nitrogen flux dynamics and protein pool sizes elicited by plant-herbivore interactions. J. Proteome Res. 11, 4947–4960. doi: 10.1021/pr300465n
Van Bockhaven, J., De Vleesschauwer, D., and Hofte, M. (2013). Towards establishing broad-spectrum disease resistance in plants: silicon leads the way. J. Exp. Bot. 64, 1281–1293. doi: 10.1093/jxb/ers329
Van Bockhaven, J., Spíchal, L., Novák, O., Strnad, M., Asano, T., Kikuchi, S., et al. (2015). Silicon induces resistance to the brown spot fungus Cochliobolus miyabeanus by preventing the pathogen from hijacking the rice ethylene pathway. New Phytol. 206, 761–773. doi: 10.1111/nph.13270
Vilela, M., Moraes, J. C., Alves, E., Santos-Cividanes, T. M., and Santos, F. A. (2014). Induced resistance to Diatraea saccharalis (Lepidoptera: Crambidae) via silicon application in sugarcane. Rev. Colomb. Entomol. 40, 44–48.
Vivancos, J., Labbe, C., Menzies, J. G., and Belanger, R. R. (2015). Silicon-mediated resistance of Arabidopsis against powdery mildew involves mechanisms other than the salicylic acid (SA)-dependent defence pathway. Mol. Plant Pathol. 16, 572–582. doi: 10.1111/mpp.12213
Wang, H.-S., Wang, C. Y., Pei-Pei, F., Bin-Fu, B., Tao, L., and Zhu-Jun, Z. (2015). Identification of two cucumber putative silicon transporter genes in Cucumis sativus. J. Plant Growth Regul. 34, 332–338. doi: 10.1007/s00344-014-9466-5
Wieczorek, M., Zub, K., Szafranska, P. A., Ksiazek, A., and Konarzewski, M. (2015). Plant-herbivore interactions: silicon concentration in tussock sedges and population dynamics of root voles. Funct. Ecol. 29, 187–194. doi: 10.1111/1365-2435.12327
Worrall, D., Holroyd, G. H., Moore, J. P., Glowacz, M., Croft, P., Taylor, J. E., et al. (2012). Treating seeds with activators of plant defence generates long-lasting priming of resistance to pests and pathogens. New Phytol. 193, 770–778. doi: 10.1111/j.1469-8137.2011.03987.x
Ye, M., Song, Y., Long, J., Wang, R., Baerson, S. R., Pan, Z., et al. (2013). Priming of jasmonate-mediated antiherbivore defense responses in rice by silicon. Proc. Natl. Acad. Sci. U.S.A. 110, E3631–E3639. doi: 10.1073/pnas.1305848110
Yoshida, Y., Sano, R., Wada, T., Takabayashi, J., and Okada, K. (2009). Jasmonic acid control of GLABRA3 links inducible defense and trichome patterning in Arabidopsis. Development 136, 1039–1048. doi: 10.1242/dev.030585
Yoshihara, T., Sogawa, M., Pathak, B. O., Juliano, B. O., and Sakamura, S. (1979). Soluble silicic acid as a sucking inhibitory substance in rice against the rice brown planthopper (Delphacidae: Homoptera). Entomol. Exp. Appl. 26, 314–322. doi: 10.1111/j.1570-7458.1979.tb02932.x
Keywords: herbivore, HIPV, effector proteins, insect–plant interactions, trophic interactions, resistance mechanisms, omics, systems biology
Citation: Reynolds OL, Padula MP, Zeng R and Gurr GM (2016) Silicon: Potential to Promote Direct and Indirect Effects on Plant Defense Against Arthropod Pests in Agriculture. Front. Plant Sci. 7:744. doi: 10.3389/fpls.2016.00744
Received: 29 January 2016; Accepted: 17 May 2016;
Published: 13 June 2016.
Edited by:Scott Nicholas Johnson, University of Western Sydney, Australia
Reviewed by:Jane DeGabriel, University of Western Sydney, Australia
Malcolm G. Keeping, South African Sugarcane Research Institute, South Africa
Copyright © 2016 Reynolds, Padula, Zeng and Gurr. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.