%A Gu,Jianwei %A Chao,Hongbo %A Gan,Lu %A Guo,Liangxing %A Zhang,Kai %A Li,Yonghong %A Wang,Hao %A Raboanatahiry,Nadia %A Li,Maoteng %D 2016 %J Frontiers in Plant Science %C %F %G English %K seed germination,Brassica napus,Proteomic analysis,Storage reserves,differentially expressed proteins (DEPs) %Q %R 10.3389/fpls.2016.01482 %W %L %M %P %7 %8 2016-October-24 %9 Original Research %+ Maoteng Li,Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology,Wuhan, China,limaoteng426@mail.hust.edu.cn %+ Maoteng Li,Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Huanggang Normal University,Huanggang, China,limaoteng426@mail.hust.edu.cn %# %! Proteomic dissection of seed germination and seedling establishment in Brassica napus %* %< %T Proteomic Dissection of Seed Germination and Seedling Establishment in Brassica napus %U https://www.frontiersin.org/articles/10.3389/fpls.2016.01482 %V 7 %0 JOURNAL ARTICLE %@ 1664-462X %X The success of seed germination and establishment of a normal seedling are key determinants of plant species propagation. At present, only a few studies have focused on the genetic control of seed germination by using a proteomic approach in Brassica napus. In the present study, the protein expression pattern of seed germination was investigated using differential fluorescence two-dimensional gel electrophoresis in B. napus. One hundred and thirteen differentially expressed proteins (DEPs) that were mainly involved in storage (23.4%), energy metabolism (18.9%), protein metabolism (16.2%), defense/disease (12.6%), seed maturation (11.7%), carbohydrate metabolism (4.5%), lipid metabolism (4.5%), amino acids metabolism (3.6%), cell growth/division (3.6%), and some unclear functions (2.7%) were observed by proteomic analysis. Seventeen genes corresponding to 11 DEPs were identified within or near the associated linkage disequilibrium regions related to seed germination and vigor quantitative traits reported in B. napus in previous studies. The expression pattern of proteins showed that heterotrophic metabolism could be activated in the process of seed germination and that the onset of defense mechanisms might start during seed germination. These findings will help generate a more in-depth understanding of the mobilization of seed storage reserves and regulation mechanisms of the germination process in B. napus.