@ARTICLE{10.3389/fpls.2016.01545, AUTHOR={Zhang, Zhong-Wei and Wu, Zi-Li and Feng, Ling-Yang and Dong, Li-Hua and Song, An-Jun and Yuan, Ming and Chen, Yang-Er and Zeng, Jian and Chen, Guang-Deng and Yuan, Shu}, TITLE={Mg-Protoporphyrin IX Signals Enhance Plant’s Tolerance to Cold Stress}, JOURNAL={Frontiers in Plant Science}, VOLUME={7}, YEAR={2016}, URL={https://www.frontiersin.org/articles/10.3389/fpls.2016.01545}, DOI={10.3389/fpls.2016.01545}, ISSN={1664-462X}, ABSTRACT={The relationship between Mg-protoporphyrin IX (Mg-Proto IX) signals and plant’s tolerance to cold stress is investigated. Arabidopsis seedlings grown for 3 weeks were pretreated with 2 mM glutamate (Glu) and 2 mM MgCl2 for 48 h at room temperature to induce Mg-Proto IX accumulation. Then cold stress was performed at 4°C for additional 72 h. Glu + MgCl2 pre-treatments alleviated the subsequent cold stress significantly by rising the leaf temperature through inducing Mg-Proto IX signals. The protective role of Glu + MgCl2 treatment was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling, and cyanide-resistant respiration. And the enhancement of cold-responsive gene expression was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling and ABA signaling, but not in the mutant of cyanide-resistant respiration. Cold stress promoted cyanide-resistant respiration and leaf total respiration exponentially, which could be further induced by the Glu + MgCl2 treatment. Mg-Proto IX signals also activate antioxidant enzymes and increase non-enzymatic antioxidants [glutathione but not ascorbic acid (AsA)] to maintain redox equilibrium during the cold stress.} }