%A Zhang,Zhong-Wei
%A Wu,Zi-Li
%A Feng,Ling-Yang
%A Dong,Li-Hua
%A Song,An-Jun
%A Yuan,Ming
%A Chen,Yang-Er
%A Zeng,Jian
%A Chen,Guang-Deng
%A Yuan,Shu
%D 2016
%J Frontiers in Plant Science
%C
%F
%G English
%K antioxidant enzyme,cold stress,Cyanide-resistant respiration,Glutathione,Mg-protoporphyrin IX signals.
%Q
%R 10.3389/fpls.2016.01545
%W
%L
%M
%P
%7
%8 2016-October-18
%9 Original Research
%+ Zhong-Wei Zhang,College of Resources, Sichuan Agricultural University,Chengdu, China,zzwzhang@sicau.edu.cn
%+ Shu Yuan,College of Resources, Sichuan Agricultural University,Chengdu, China,zzwzhang@sicau.edu.cn
%#
%! Mg-proto IX signals in cold stress tolerance
%*
%<
%T Mg-Protoporphyrin IX Signals Enhance Plant’s Tolerance to Cold Stress
%U https://www.frontiersin.org/articles/10.3389/fpls.2016.01545
%V 7
%0 JOURNAL ARTICLE
%@ 1664-462X
%X The relationship between Mg-protoporphyrin IX (Mg-Proto IX) signals and plant’s tolerance to cold stress is investigated. Arabidopsis seedlings grown for 3 weeks were pretreated with 2 mM glutamate (Glu) and 2 mM MgCl2 for 48 h at room temperature to induce Mg-Proto IX accumulation. Then cold stress was performed at 4°C for additional 72 h. Glu + MgCl2 pre-treatments alleviated the subsequent cold stress significantly by rising the leaf temperature through inducing Mg-Proto IX signals. The protective role of Glu + MgCl2 treatment was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling, and cyanide-resistant respiration. And the enhancement of cold-responsive gene expression was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling and ABA signaling, but not in the mutant of cyanide-resistant respiration. Cold stress promoted cyanide-resistant respiration and leaf total respiration exponentially, which could be further induced by the Glu + MgCl2 treatment. Mg-Proto IX signals also activate antioxidant enzymes and increase non-enzymatic antioxidants [glutathione but not ascorbic acid (AsA)] to maintain redox equilibrium during the cold stress.