%A Zhang,Zhong-Wei %A Wu,Zi-Li %A Feng,Ling-Yang %A Dong,Li-Hua %A Song,An-Jun %A Yuan,Ming %A Chen,Yang-Er %A Zeng,Jian %A Chen,Guang-Deng %A Yuan,Shu %D 2016 %J Frontiers in Plant Science %C %F %G English %K antioxidant enzyme,cold stress,Cyanide-resistant respiration,Glutathione,Mg-protoporphyrin IX signals. %Q %R 10.3389/fpls.2016.01545 %W %L %M %P %7 %8 2016-October-18 %9 Original Research %+ Zhong-Wei Zhang,College of Resources, Sichuan Agricultural University,Chengdu, China,zzwzhang@sicau.edu.cn %+ Shu Yuan,College of Resources, Sichuan Agricultural University,Chengdu, China,zzwzhang@sicau.edu.cn %# %! Mg-proto IX signals in cold stress tolerance %* %< %T Mg-Protoporphyrin IX Signals Enhance Plant’s Tolerance to Cold Stress %U https://www.frontiersin.org/articles/10.3389/fpls.2016.01545 %V 7 %0 JOURNAL ARTICLE %@ 1664-462X %X The relationship between Mg-protoporphyrin IX (Mg-Proto IX) signals and plant’s tolerance to cold stress is investigated. Arabidopsis seedlings grown for 3 weeks were pretreated with 2 mM glutamate (Glu) and 2 mM MgCl2 for 48 h at room temperature to induce Mg-Proto IX accumulation. Then cold stress was performed at 4°C for additional 72 h. Glu + MgCl2 pre-treatments alleviated the subsequent cold stress significantly by rising the leaf temperature through inducing Mg-Proto IX signals. The protective role of Glu + MgCl2 treatment was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling, and cyanide-resistant respiration. And the enhancement of cold-responsive gene expression was greatly compromised in the mutants of Mg-Proto IX synthesis, Mg-Proto IX signaling and ABA signaling, but not in the mutant of cyanide-resistant respiration. Cold stress promoted cyanide-resistant respiration and leaf total respiration exponentially, which could be further induced by the Glu + MgCl2 treatment. Mg-Proto IX signals also activate antioxidant enzymes and increase non-enzymatic antioxidants [glutathione but not ascorbic acid (AsA)] to maintain redox equilibrium during the cold stress.