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Original Research ARTICLE

Front. Plant Sci., 27 March 2017 | https://doi.org/10.3389/fpls.2017.00405

A Conserved Potential Development Framework Applies to Shoots of Legume Species with Contrasting Morphogenetic Strategies

  • INRA, UR4, URP3F, BP6, Lusignan, France

A great variety of legume species are used for forage production and grown in multi-species grasslands. Despite their close phylogenetic relationship, they display a broad range of morphologies that markedly affect their competitive abilities and persistence in mixtures. Little is yet known about the component traits that control the deployment of plant architecture in most of these species. During the present study, we compared the patterns of shoot organogenesis and shoot organ growth in contrasting forage species belonging to the four morphogenetic groups previously identified in herbaceous legumes (i.e., stolon-formers, rhizome-formers, crown-formers tolerant to defoliation and crown-formers intolerant to defoliation). To achieve this, three greenhouse experiments were carried out using plant species from each group (namely alfalfa, birdsfoot trefoil, sainfoin, kura clover, red clover, and white clover) which were grown at low density under non-limiting water and soil nutrient availability. The potential morphogenesis of shoots characterized under these conditions showed that all the species shared a number of common morphogenetic features. All complied with a generalized classification of shoot axes into three types (main axis, primary and secondary axes). A common quantitative framework for vegetative growth and development involved: (i) the regular development of all shoot axes in thermal time and a deterministic branching pattern in the absence of stress; (ii) a temporal coordination of organ growth at the phytomer level that was highly conserved irrespective of phytomer position, and (iii) an identical allometry determining the surface area of all the leaves. The species differed in their architecture as a consequence of the values taken by component traits of morphogenesis. Assessing the relationships between the traits studied showed that these species were distinct from each other along two main PCA axes which explained 68% of total variance: the first axis captured a trade-off between maximum leaf size and the ability to produce numerous phytomers, while the second distinguished morphogenetic strategies reliant on either petiole or internode expansion to achieve space colonization. The consequences of this quantitative framework are discussed, along with its possible applications regarding plant phenotyping and modeling.

Introduction

Numerous forage legumes contribute to temperate grasslands and help to supply high-quality protein-rich feed for ruminants, while reducing the need for nitrogen fertilizers (Suter et al., 2015; Vertès et al., 2015), preserving water quality (Owens et al., 1994; Russelle et al., 2001) and mitigating greenhouse gas emissions (Jensen et al., 2012). Most of these legume species are grown in a mixture with perennial grasses in order to take advantage of the ecological and nutritional complementarities of the two functional groups (Nyfeler et al., 2011; Gaba et al., 2015). However, a long-acknowledged problem of multi-species grasslands is the lack of persistence of the legume component over time and a less predictable forage quality when compared with pure grasses or annual forages (Beuselinck et al., 1994; Schwinning and Parsons, 1996). Competition for resources and crop management have been shown to be of considerable importance to regulating the proportion of legumes in grassland communities (Sheaffer, 1989; Beuselinck et al., 1994), but little is known about the mechanisms by which a legume species prevails within a particular community or environment. To date, the search for combinations of traits predictive of legume performance in a mixture has been limited to a few widely-grown mixtures (e.g., white clover-perennial ryegrass or alfalfa-tall fescue: Davies, 2001; Annicchiarico et al., 2015; Maamouri et al., 2015), and only a few studies have sought to explore the role of the diversity of plant forms using multi-trait approaches (e.g., Fort et al., 2015 on root traits; Kraft et al., 2015).

The legume family presents spectacular morphological and life-history trait diversity (Lewis et al., 2005; LPWG, 2013). Considering the temperate herbaceous genus only, a remarkable range of plant structures and pedoclimatic adaptations has been reported (Forde et al., 1989; Scott et al., 1989). The morphogenesis of shoots determines plant architecture and light capture (Valladares and Niinemets, 2007), which are critical to inferring the outcome of competition for light (Caldwell, 1987). It also determines the position of shoot meristems and contributes to how different species tolerate grazing and mowing (Briske, 1996; Smith et al., 2000). Differences in leaf surface area, plant height and other architectural features that affect the spatial distribution of leaves (e.g., phenology, branching patterns, dimensions of spacing organs, etc.) have been shown to be drivers of competitive success in grass-legume mixtures (Louarn et al., 2012; Barillot et al., 2014). However, little is known about the elementary traits that promote leaf area expansion and height increments in most herbaceous species, especially regarding the temporal aspects of morphogenesis. Unlike grasses, for which a regular developmental scheme was identified and mobilized a long time ago to compare species and genotypes (Simon and Lemaire, 1987; Lafarge and Durand, 2013), no obvious pattern has emerged from comparisons of shoots from crown-, stolon- and rhizome-forming legumes used for forage production (Forde et al., 1989; Thomas, 2003; Figure 1).

FIGURE 1
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Figure 1. Diagrammatic representation of the vegetative development of forage legumes from different morphogenetic groups (left) and images of the corresponding species tested (right).

As parts of a modular organism, the common feature of all plant shoots is that they are built from elementary subunits called phytomers (White, 1979). Shoot morphogenesis thus arises from the initiation of new phytomers by shoot meristems, from the expansive growth of the individual organs produced and from the differentiation of support tissues. Like many other dicotyledonous plants (Seleznyova et al., 2002; Lebon et al., 2006), forage legumes are characterized by shoots with complex and highly branched structures (e.g., white clover: Thomas, 1987; Gautier et al., 2000; Thomas et al., 2014; red clover: Taylor and Quesenberry, 1996). Considerable variability of shoot development is usually observed in dense stands (Gosse et al., 1988; Van Minnebruggen et al., 2015). However, architectural and developmental analyses have proved to be powerful tools to classify shoot types and quantify branching and potential shoot development in different species and varieties, based on stochastic (Costes and Guédon, 2002; Louarn et al., 2007) and deterministic (Turc and Lecoeur, 1997) approaches. Although, using different methods, regular organogenetic patterns have been found in alfalfa (Baldissera et al., 2014), red clover (Van Minnebruggen et al., 2014), and Medicago truncatula (Moreau et al., 2006), suggesting that a generalized description could be envisioned for these species.

Similarly, the complexity of plant growth, distributed between individual organs that all vary in size and shape, has been shown to be driven by a number of common determinants. For instance, the temporal sequences of organ growth generally appear to be coordinated at a supra-organ level. In grasses, changes to the phases of leaf growth are triggered by emergence of the previous leaves (Skinner and Nelson, 1994; Fournier et al., 2005; Louarn et al., 2010). In many other species, stable thermal time calendars of development have been reported at the plant and phytomer levels in the absence of stress (Granier et al., 2002; Lecoeur, 2010; Demotes-Mainard et al., 2013). Furthermore, the ultimate sizes attained by organs are highly heritable traits (Annicchiarico et al., 1999), which present relatively conserved ontogenic patterns along the stem under controlled conditions (Ross, 1981). Overall, organogenetic and expansive growth characteristics define and—by their ongoing interactions constrain—the shoot morphogenesis of any particular plant genotype. Trade-offs very often occur (e.g., in grasses between leaf growth and tillering; Nelson, 2000; Barre et al., 2015), which therefore makes it worthwhile analysing these two aspects of morphogenesis together.

In order to facilitate the future characterization of shoot morphogenesis in different legume species and cultivars, this paper was designed to: (i) analyse the elementary traits controlling plant leaf area and height in a selection of six herbaceous species, contrasting in terms of their growth habits and architectures, (ii) challenge the existence of a common framework for vegetative development under non-limiting growing conditions, and (iii) assess the relationships between the traits studied and the concomitant occurrence of trait values which might indicate ontogenic trade-offs in the morphogenesis of shoots.

Materials and Methods

Plant Materials and Growing Conditions

Three experiments were carried out in a greenhouse at INRA Lusignan, France (46°26′ N, 0°07′ E). The two first took place from February to April in 2014 and 2015, whereas the third experiment was carried out from November 2016 to February 2017. Plants of alfalfa (Medicago sativa L. cv. Timbale; hereinafter referred to as A), white clover (Trifolium repens L. cv. Giga; WC), red clover (Trifolium pratense L. cv. Formica; RC), and sainfoin (Onobrychis viciifolia Scop. cv. Canto; SF) were studied during the two first experiments. In addition, plants of birdsfoot trefoil (Lotus corniculatus L. cv. Leo; BT) and kura clover (Trifolium ambiguum cv. Sevanskij; KC) were grown in the second and third years. Overall, the six species selected covered a wide range of shoot growth habits and the four morphogenetic groups previously reported among perennial herbaceous legumes from temperate areas (Forde et al., 1989; Thomas, 2003; Figure 1) and adapted to contrasting ecological niches (Scott et al., 1989).

In each experiment, seeds from each species were germinated for 48 h in Petri dishes at 25°C in the dark. In addition, clones propagated from rhizome cuttings were selected on 2-years-old plants and used for TK in the third experiment. The seedlings were planted 0.3 m × 1 m apart in custom-built 10 L boxes containing sterile potting mix, sand and brown soil (1:1:1, v/v/v). For each species, the plants were grown in isolation (3.3 plant.m−2) until the end of the vegetative stage, according to a randomized block design with four (Exp. 1 and 2) to five (Exp. 3) replicates by species. Irrigation and fertilization were provided throughout the experiments with a drip system 5 cm distant from the plants that delivered 300 mL.d−1 of complete nutrient solution (Gastal and Saugier, 1986). The nitrogen concentration of the solution (8 mM) was non-limiting for growth and prevented the nodulation of roots in all the legume species. The greenhouse was heated and a photoperiod of 16 h was maintained by means of 400-Watt HQI lamps (Supplementary Table S1).

Plant Measurements

To analyse the temporal development of photosynthetic surfaces and their spatial distributions in each species, the organogenesis and expansion of shoot organs were measured for all the plants for a period of about 70 days. The terminology used to refer to the different shoot axes was based on the architectural descriptions of alfalfa and white clover (Moulia et al., 2000; Baldissera et al., 2014) and is summarized in Supplementary Figure S1A.

The numbers of axes and numbers of phytomers on the different axes were counted weekly. A decimal scale was used to account for phytomers with unfolded leaves (Maître et al., 1985). Furthermore, several groups of three consecutive phytomers were selected for daily growth measurements on both primary and secondary axes. Ranks 5, 6, 7 and 11, 12, 13 were followed on the main axis. Ranks 3, 4, 5 were characterized on primary axes and branches. The length of each organ (i.e., leaflet, petiole, internode in all species; stipule in birdsfoot trefoil; Supplementary Figure S1B) was measured every day with a ruler until no further growth was noted over 4 consecutive days.

At the end of each experiment, the final length and width of each mature organ along the primary axes was measured. In experiments 1 and 2, a sub-sample of phytomers was used in each species to determine the leaf area of individual organs. Leaves and stipules of various sizes and positions were scanned (Konica Minolta C352/C300, Konica Minolta Sensing, Osaka, Japan) and their area was measured by image analysis (ImageJ software, http://rsbweb.nih.gov/ij/). The height of plants, the total plant leaf area and the total dry weight of plants were also measured.

Meteorological Measurements and Thermal Time Calculations

Relative humidity was measured using a capacitive hygrometer (HMP35A Vaisala, Oy, Helsinki, Finland) and air temperature with copper-constantan thermocouples placed in a ventilated radiation shield at the center of the greenhouse. Photosynthetic photon flux density (PPFD) was also measured by means of PPFD sensors placed above each experimental bloc within the greenhouse. All data were stored in a datalogger (CR10X, Campbell Scientific Ltd.), with measurements taken every 30 s and an average calculated over 15 min. The data are summarized for each experiment in Supplementary Table S1.

Thermal time (TT) was calculated as the integral of a non-linear beta function of temperature (T) as proposed by Zaka et al. (2017):

f(T)=(T-TminTref-Tmin)q.(Tmax-TTmax-Tref)    (1)
TT=t0tmax[0,(Tref-Tbase).f(T)].dt    (2)

The equation has three parameters: the minimum (Tmin) and maximum (Tmax) temperatures at which development occurs and q, a shape parameter. In addition, Tref accounts for a fixed reference temperature (20°C) and Tbase (5°C) for a common base temperature used to scale time units from equivalent days at the reference temperature into degree days (°Cd). The parameters used for the different species were derived from the literature and are presented in Supplementary Table S2.

Data Analysis

All calculations and statistical tests were performed using R software (version 3.1.2; R Development Core Team, 2014). Rates of leaf appearance were calculated for each axis with three or more unfolded leaves by linear regression between thermal time and the number of visible phytomers. Phyllochrons were calculated as the reciprocal of the leaf appearance rate.

The temporal growth of plant organs was analyzed using a three-parameter logistic function (Equation 3) fitted to the time series of organ growth measurements:

L(t)/Lmax=11+e-s.(t-t50)    (3)

where the s and t50 parameters represent the steepness and time delay at mid-organ expansion and Lmax the final organ length. By convention, all the organs within a phytomer were analyzed with respect to the leaf appearance (i.e., t50 of leaflets = 0) and time was expressed in phyllochrons to aggregate growth series from axes with different developmental rates. The duration of organ expansion between 5 and 95% of its final dimension (d95) was derived for each organ from the s parameter as follow:

d95=-2.ln(0.05/0.95)/s    (4)

For each phytomer, branching probability was calculated at a given date as the ratio between the number of branches with an outburst at this position and the total number of plants in the treatment. The relationship between branching probability and phytomer position was characterized on the main and primary axes using a logistic function similar to Equation 3.

Significant differences between the means of plant traits were tested by performing analyses of variance (“aov” procedure). Analyses of covariance (ANCOVA, lm procedure) were used to test simultaneously for the effects of continuous and categorical variables and to compare the slopes and intercepts of linear relationships. Principal component analysis (PCA) was performed to assess the relationships between shoot morphogenetic parameters using the ade4 package (13 parameters, 50 individuals).

Results

Organogenesis on the Main Axis

New phytomers appeared on the main axis at a constant rate during the vegetative phase, resulting in a linear relationship between the total number of phytomers on an axis and thermal time in all the species studied (R2 > 0.95; Figure 2). The leaf appearance rate differed markedly between species (ANCOVA, p < 0.001) and was conserved between experimental years. Phyllochrons ranged from 32.6°C day in birdsfoot trefoil to 64.2°C day in Kura clover, with a stable pecking order (BT-A<SA<WC<RC<KC).

FIGURE 2
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Figure 2. Timing of leaf (filled symbols) and branch (open symbols) appearance on the main axis of alfalfa (A), white clover (WC), red clover (RC), sainfoin (SF), birdsfoot trefoil (BT), and kura clover (KC). Circles: first experiment (2014); squares: second experiment (2015); diamonds: third experiment (2016). GDD: growing °C days. Vertical bars indicate standard deviation (4 to 5 replicates).

Branching

The timing of budburst of axillary branches on the main axis appeared to be linearly related to thermal time (R2 > 0.79; Figure 2). A short lag period was observed systematically between the appearance of a phytomer and axillary budburst, so that phytomer appearance and the burst of the corresponding axillary bud was always separated by approximately two (A) to 8 (KC) phyllochrons, depending on the species. This delay of sylleptic branching remained constant over the period of observation in all the species, at all the positions (ANCOVA, p > 0.77). Accordingly, branching rates strongly differed between species and followed an inverse phyllochron order (BT-A>SA-WC>RC>KC). Furthermore, sylleptic branching appeared to be systematic on the main axis and primary axes (i.e., the branching probability ultimately reached 1 for all the phytomers after a certain delay; Figure 3), making it a deterministic process in isolated plants subjected to weak competition for light.

FIGURE 3
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Figure 3. Branching probability with respect to phytomer position expressed from the apex for alfalfa (A), white clover (WC), red clover (RC), sainfoin (SF), birdsfoot trefoil (BT) and kura clover (KC). Circles: first experiment (2014); squares: second experiment (2015); diamonds: third experiment (2016). Different symbols indicate different sampling dates. Logistic curves were fitted using Equation 3.

Organogenesis on the Primary and Secondary Axes

The development of primary (emerging from the collar zone) and secondary axes is further presented in Figure 4. As for the main axis, primary and secondary axes produced new phytomers at a constant rate in thermal time. Leaf appearance rates on the primary axes were similar to the main axis in A and BF, but differed in the other species (ANCOVA, p < 0.01). In white and red clovers, the primary axes developed more rapidly than the main axis, whereas the reverse was observed in sainfoin and Kura clover. Comparatively, the secondary axes displayed a much slower rate of development than primary axes (i.e., in A and BT, ANCOVA, p < 0.001). No significant effect of the topological position was found on the rate of development of primary and secondary axes in any of the six species (ANCOVA, p > 0.20).

FIGURE 4
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Figure 4. Leaf appearance on the primary (filled circles) and secondary (open circles) axes for alfalfa (A), white clover (WC), red clover (RC), sainfoin (SA), birdsfoot trefoil (BT), and kura clover (KC). Dashed lines indicate the leaf appearance rate on the main axis (Figure 3). Bold and black lines indicate the linear regressions for the primary and secondary axes, respectively. Only axes with at least 3 unfolded leaves were plotted. GDD: growing °C days.

Coordination of Organ Growth within a Phytomer

Once initiated, the different organs within a phytomer displayed highly conserved kinetics of expansion when expressed according to axis development (Figure 5). A strict scheduling organized the sequence of the onset of organ growth and the subsequent relative expansion of the different organs. For all of them, the time sequence of organ elongation as a function of phyllochronic time was approximated correctly using a sigmoid function (Equation 3). No significant effects of phytomer rank or order were found with respect to growth delay and maximum relative elongation rate in any of the species (d and s parameters, ANOVA, p > 0.51), suggesting a coordination of organ growth was conserved irrespective of the phytomer within each species.

FIGURE 5
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Figure 5. Kinetics of relative growth for leaflets (black filled circles), petioles (gray filled circles), internodes (open circles) and stipules (open triangles) in alfalfa (A), white clover (WC), red clover (RC), sainfoin (SF), birdsfoot trefoil (BT), and kura clover (KC). Red lines represent the fits of a logistic function (Equation 3) for each organ type.

On the other hand, the different species displayed very dissimilar organ growth coordination patterns. The differences were particularly marked concerning the maximum elongation rates and the duration of phytomer expansion (ANOVA, p < 0.001). Some species, such as A and BT, typically presented a slow expansion of organs relative to axis development, and phytomer growth lasting for five phyllochrons. By contrast, white clover displayed rapid expansion and a total duration limited to 2.5 phyllochrons. The species also differed in the timing and relative order of organ growth. In most cases, leaf elements elongated first (leaflets > (stipules) > petiole), followed by internode elongation. However, in white clover, the order was reversed and internodes were first to complete their elongation.

Organ Dimensions at Maturity

For each species, the size ultimately attained by individual organs at maturity depended on the phytomer position. Figure 6 presents the changes in relative organ dimensions along the primary axes. Irrespective of species and organ type, typical vegetative shoot patterns displayed profiles that first increased in size in line with phytomer ranks, and then stabilized at a plateau value, or even decreased. Interestingly, the relative profiles remained unchanged between the experimental years and were characteristic of isolated plants in a given species. Rank by rank comparisons of relative dimensions yielded identical mean values in 50 out of 53 cases for leaves, and 57 out of 58 cases for petioles/internodes (Student test, p > 0.5). For each organ type, the species differed in terms of the position of the phytomer at which maximum organ size was achieved. Concerning individual leaf size for instance, up to 12 phytomers were produced before reaching the peak in SF, but only 7–8 were necessary in WC or A.

FIGURE 6
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Figure 6. Relative leaf area (first row) and relative length of the main spacing organ (second row, G and K: internodes; H,I,J, and L: petioles) for the first 14 phytomers on the primary axes for alfalfa (A), white clover (WC), red clover (RC), sainfoin (SF), birdsfoot trefoil (BT) and kura clover (KC). Vertical bars indicate standard deviation (4 to 5 replicates). Different colors indicate different experimental years. Arrows indicate the position of the rank used for the normalization of relative length and relative area. The main spacing organs were internodes in A, BF and SF and petioles in WC, RC, and KC.

As for the actual size of organs, maximum leaf area and petiole length differed in all the species, but the ranking between species remained unaffected by the experimental year (Table 1). Significant differences were observed between years only for the maximum internode length in some of the species. This trait was slightly smaller during the second experiment in white clover and alfalfa, but not in red clover and sainfoin.

TABLE 1
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Table 1. Maximum organ dimensions observed during experiments 1 and 2 for alfalfa (A), white clover (WC), red clover (RC), sainfoin (SA), birdsfoot trefoil (BT), and kura clover (KC).

Leaf Allometry

Leaf size and shape varied considerably between species and phytomers. Except in sainfoin, the leaves were all trifoliolate from the second leaf on. Sainfoin presents compound leaves, with a number of leaflets that can increase up to 25 (Table 1). Despite the variability in their shapes, leaves from all the species complied with a single allometric relationship (r2 = 0.96; Supplementary Figure S2) linking leaf area (LA) with central leaflet length (L), central leaflet width (l) and the number of leaflets (n):

LA=0.694×L×l×n    (5)

Relationships between Shoot Morphogenetic Traits

The possibility of defining sets of trait values occurring concomitantly was assessed by performing PCA on the dataset defined by the major morphogenetic traits characterized during the two experiments (Table 2). The first component of this PCA (Figure 7A), which explained almost half of total variance (47.8%), was mainly determined by leaf growth traits (MAXpet, MAXlf, duration of leaf growth) and by the organogenesis of the main and primary axes (Phy0, Br1). It expressed an antagonism between the rate of phytomer production and the size and duration of expansion of leaf elements. Component 2, on the other hand, was mainly correlated to traits controlling the kinetics of internode expansion (t50in) and to the development of secondary branches (ram_dist, Phy2). The growth of internodes was the most able in discriminating the species in terms of their growth coordination calendar. Petiole and leaflet expansions were tightly related in all the species, but internodes could expand either before or after leaf elements. In the plane containing component 1 and component 3 (not shown), it was seen that the third component was mainly correlated to the maximum length of internodes (MAXin). Phy2 was also positively correlated with this third component, showing that species with the longest nodes also displayed a more vigorous development of secondary branches.

TABLE 2
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Table 2. Variables used in the Principal Component Analysis presented in Figure 7.

FIGURE 7
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Figure 7. Principal component analysis. (A) Variables (morphogenetic traits) located on the plane defined by components 1 (horizontally) and 2 (vertically), and (B) individual scatterplot for component 1 and 2. See Table 2 for the names of the traits. Different colors represent individuals from different species: alfalfa (a, red), white clover (wc, blue), red clover (rc, black), sainfoin (sf, purple), birdsfoot trefoil (bt, yellow), and kura clover (kc, green). Ellipses of dispersion represent the four morphogenetic groups (stolon-formers, S; rhizome-formers, R; crown-formers tolerant to defoliation, Ct; crown-formers intolerant to defoliation, C).

Discussion

Potential Shoot Morphogenesis Followed a Set of Deterministic Rules Common to the Six Legume Species

The morphogenesis of shoots can lead to highly differentiated plant architectures in perennial herbaceous legumes (Forde et al., 1989; Thomas, 2003). However, as species differ in their branching complexity and in the size, position and shape of their shoot organs, our results highlighted the fact that they also share a number of determinants regarding the organogenesis and growth of phytomers, the building blocks of plant architecture. The striking differences between the species we studied emerged within the framework of a common and quite delimited pattern of vegetative growth and development. This framework involved: (i) a deterministic branching pattern and a regular development of shoot axes, (ii) a coordination of organ growth at the phytomer level, and (iii) a conserved allometry of leaf shapes.

Concerning shoot organogenesis, the existence of a generalized pattern of development was supported by the fact that all the species complied with the proposed classification of shoot axes in three categories. The main axis displayed developmental characteristics distinct from primary axes (that subsequently emerged close to the plant collar) and secondary branches (emerging from axillary buds out of the collar zone), each type presenting identical phyllochrons during the different experiments and for axes at different topological positions. In two of the species (namely A and BT), the main axis and primary axes presented similar characteristics, making the classification even potentially simpler in legumes where the main axis can elongate. Given the important differences existing among dicots in terms of branching behavior and apical dominance (McSteen and Leyser, 2005), such regularity in the organogenetic process was not necessarily to be expected. Sylleptic shoot branching often occurs as a seemingly stochastic process, under the dependence of internal and environmental regulatory signals (Génard et al., 1994; Seleznyova et al., 2002; Rameau et al., 2015). Complex trophic and hormonal interplays can result in branches of the same order expressing very different characteristics depending on their position in the branching system (Lebon et al., 2004; Moreau et al., 2007), or presenting properties that change over time (e.g., increasing phyllochron, Barillot et al., 2012). Overall, the constant phyllochrons and branching delays we reported in the species we studied appeared to constitute a very simple way to characterize potential organogenesis. These characteristics may hold true mainly because we focused our attention on the period of vegetative development, during which little competition from other plant parts occurs. However, these observations were consistent with previous reports regarding herbaceous legumes (e.g., in alfalfa, Baldissera et al., 2014), and will make the characterization of new species and genotypes easier in this group of species.

The temporal coordination of organ growth also appeared to be highly conserved, irrespective of phytomer position, in all the species. Such stable calendars of expansion had previously been reported for leaves and internodes on the primary axis of different dicotyledonous species (Granier et al., 2002; Demotes-Mainard et al., 2013). Interestingly, our results suggest that this could be extended to axes of different types once time is normalized by the rate of leaf appearance on each axis (i.e., phyllochronic time). Such an approach is new for dicots but had been used successfully to account for the flexible growth pattern of grass leaves, constantly adapting to the timing of leaf appearance (Fournier et al., 2005; Zhu et al., 2014). It could probably be applied to account for the relationship between the rate of phytomer production and the timing and duration of organ growth within a phytomer on a broad range of species.

The allometric relationship found between leaf area and the product of leaf length and leaf width is currently applied in numerous species (Schwarz and Kläring, 2001; Antunes et al., 2008; Baldissera et al., 2014). Accounting for the number of leaflets was sufficient to encompass the different species within the same relationship in our study (Equation 5). This indicates that a common shape coefficient could be applied to expanded leaflets from the different legume species (Prévot et al., 1991). However, considering both leaf length and leaf width was a necessity to establish this common relationship, because some dissociation exists between the longitudinal and lateral expansion of leaf laminas (e.g., the former being mediated by brassinosteroids without any effect on the latter; Nakaya et al., 2002), resulting in length to width ratios that vary as a function of phytomer position and species.

On the other hand, no clear pattern governing organ dimension at maturity, and which could encompass all the species, emerged at the axis level. Typical leaf and internode length profiles reached a maximum value at an intermediate position along the axis, a situation that is relatively ubiquitous in vascular plants (Allsopp, 1967; Villani and Demason, 2000). These positions were similar between experiments in a given species, but they changed dramatically depending on organ type and between species. Furthermore, the maximum dimension attained by internodes changed between two of the experiments. As water and nutrients were supplied without restriction, these differences could have resulted from the slightly different light regimes prevailing in the different years, or could have been caused by greater evaporative demand affecting organ expansion in 2015 (Supplementary Table S1, Tardieu et al., 2005).

Differences in Trait Values Determined the Contrasting Morphogenetic Strategies

Despite this common developmental pattern, the species sampled did produce contrasting shoot architectures. Even without considering geometric features (such as leaf angles, shoot bearing, etc.), the values for component traits of shoot morphogenesis and which accounted for phytomer production (phyllochrons, delay of branching) and organ growth (delays of expansion, relative expansion rate, maximum organ dimension) explained the emergence of four morphogenetic groups (Figure 7B) that partially matched those identified by Thomas (2003). Only kura clover, which is in fact a rhizome-forming species, was closely related with two crown-forming species producing a short main axis (RC and SF). As previously shown (Genrich et al., 1998; Black et al., 2006), kura clover was very slow to develop and did not express its rhizomatous growth habit during the period studied. The primary axes characterized from rhizome cuttings in the third experiments did not differ from those emerging from collar in the second experiment. The developmental pattern of KC shoots thus appeared very similar to red clover in our conditions, explaining the close classification of the two species.

The main morphogenetic traits associated with this classification were distinguished along two PCA axes. A first dimension represented the strategies of leaf area production, reliant on either the production of numerous small leaves or the expansion of a fewer large leaves. Such a trade-off between growth and development is common during morphogenesis and has, for instance, been reported in grass tillers (leaf length vs. phyllochron and tillering rate; Gautier et al., 1999; Nelson, 2000), and in roots (root elongation rate vs. branching ability: Pagès, 2014). In forage legumes, this could be a key element in controlling the rate of regrowth in a mixture after defoliation (e.g., in white clover and alfalfa: Davies, 2001; Annicchiarico, 2003; Annicchiarico et al., 2015). A second dimension mainly distinguished species in terms of their growth coordination patterns, promoting either early internode growth or the early growth of leaf elements (including petioles) as a primary option to colonize space and expand vegetative structures. All species but white clover favored the growth of leaf elements, which is consistent with a pre-emptive strategy for light acquisition that could be expected in crown-formers with limited prospecting ability. Under such local competition, rapid plant leaf area development indeed appears to be the most successful trait to capture light (Louarn et al., 2012). On the other hand, white clover develops primary axes by first sensing its environment through internode elongation. This particular trait could be an adaptation to achieve horizontal prospection for light gaps, as white clover tends to be a light foraging species rather than a competitive one (de Kroons and Hutchings, 1995). Similar growth coordination patterns are observed in lianas (e.g., grapevine, Louarn, 2005) and in invasive species sprouting through long cane emission (e.g., rubus sp., Amor, 1974), thus making such an hypothesis plausible.

A degree of redundancy between several of the morphogenetic traits was apparent (e.g., high correlations between petiole and leaflet size, or between the size of these organs and their duration of expansion), which suggests opportunities to simplify the characterization of morphogenetic strategies. Overall, however, the combined values of these traits enabled the discrimination and classification of the species as a function of their known ecological behaviors.

Interests and Limitations of Such a Framework for Plant Phenotyping and Modeling

Identifying a robust framework that enables the description, analysis and prediction of plant morphogenesis is central to developing efficient phenotyping approaches for breeding (Granier et al., 2002; Moreau et al., 2006) and the diagnosis of plant stress (e.g., Pellegrino et al., 2005). The framework for potential morphogenesis we have discussed above represents a first step toward achieving both of these goals with respect to perennial herbaceous legumes, many of which have been little characterized to date. Setting up such a potential framework in absence of competition is particularly important to identify ontogenic patterns of development and to disentangle the contradictory effects of neighbors on plant morphology and resource acquisition (Lemaire and Millard, 1999).

Clearly, our approach is currently limited to the vegetative period of development, and the interactions which may take place during flowering or the development of reproductive organs at later stages are not taken into account. In most forage species, regular harvests of shoot biomass over a season involve several cuts of primary shoot axes, and a succession of vegetative development from crown and the remaining lateral buds, thus making particularly relevant the vegetative framework presented. However, in prostrate species such as white clover, or in those with a terminal flowering (Thomas, 2003), interactions with the reproductive cycle will occur at some point, and integrating a reproductive dimension in the framework would most likely improve its robustness (e.g., Moreau et al., 2007). Similarly, the development of nodal roots has been shown to interact with shoot organogenesis in aging clover plants (Thomas et al., 2014), and a whole plant appraisal of morphogenesis, considering both shoot and root morphogenetic traits (Pagès, 2014) would deserve exploration. In any case, the set of deterministic rules identified in this study could serve as an initial benchmark for the future development of a more comprehensive framework. As a potential morphogenetic model, it could also serve as a baseline to analyse the responses of vegetative development to environmental stresses (Belaygue et al., 1996; Lebon et al., 2006; Baldissera et al., 2014), and to compare on a quantitative basis a broad range of species and genotypes with respect to their morphogenetic strategies (Louarn et al., 2007).

At present, the framework provides a generic approach to break down the component traits of competitive ability aboveground (i.e., the components of leaf area expansion and height acquisition: Barillot et al., 2011; Louarn et al., 2012). This is of particular value because: (i) the genetic component of competitive ability has been shown to be more tightly related to component traits of morphogenesis than to integrated traits (Annicchiarico et al., 1999), (ii) such a framework could help to highlight specific combinations of traits associated with a particular morphogenetic group, even in species for which the amount of background studies is limited, and (iii) the knowledge of component traits associated with competitive ability is expected to increase the efficiency of pure stand selection that targets mixed stand conditions, thus offering opportunities to reduce costs during the selection process for multi-species grasslands (Annicchiarico, 2003). Finally, such a quantitative approach could also readily be integrated as a component in mixed grassland models (Soussana et al., 2012), so as to enable better consideration of the diversity of forms that legume components could accommodate, and to improve the prediction of their persistence in mixtures.

Author Contributions

LF and GL designed the experiments, conducted measurements and performed data analyses. IL and GL rose funding for these research. All of the authors contributed to writing the manuscript.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

This study was supported by the Agence Nationale de la Recherche (PRAISE project, ANR-13-BIOADAPT-0015), the Poitou Charentes Regional Council (PhD fellowship for LF) and INRA's Environment and Agronomy Division (Transfert-N Project, PhD fellowship for LF). The authors would like to thank M. Demon, A. Eprinchard, C. Perrot, E. Roy, E. Rivault, A. Philipponneau, and J. P. Terrasson for their assistance with the experiments.

Supplementary Material

The Supplementary Material for this article can be found online at: https://www.frontiersin.org/article/10.3389/fpls.2017.00405/full#supplementary-material

References

Allsopp, A. (1967). Heteroblastic development in vascular plants. Adv. Morphog. 6, 127–171. doi: 10.1016/b978-1-4831-9953-5.50008-1

PubMed Abstract | CrossRef Full Text | Google Scholar

Amor, R. L. (1974). Ecology and control of blackberry (Rubus fruticosus L. agg.) II. Reproduction. Weed Res. 14, 231–238. doi: 10.1111/j.1365-3180.1974.tb01047.x

CrossRef Full Text | Google Scholar

Annicchiarico, P. (2003). Breeding white clover for increased ability to compete with associated grasses. J. Agric. Sci. 140, 255–266. doi: 10.1017/S0021859603003198

CrossRef Full Text | Google Scholar

Annicchiarico, P., Barrett, B., Brummer, E. C., Julier, B., and Marshall, A. H. (2015). Achievements and challenges in improving temperate perennial forage legumes. CRC. Crit. Rev. Plant Sci. 34, 327–380. doi: 10.1080/07352689.2014.898462

CrossRef Full Text | Google Scholar

Annicchiarico, P., Piano, E., and Rhodes, I. (1999). Heritability of, and genetic correlations among, forage and seed yield traits in ladino white clover. Plant Breed. 118, 341–346. doi: 10.1046/j.1439-0523.1999.00387.x

CrossRef Full Text | Google Scholar

Antunes, W. C., Pompelli, M. F., Carretero, D. M., and DaMatta, F. M. (2008). Allometric models for non-destructive leaf area estimation in coffee (Coffea arabica and Coffea canephora). Ann. Appl. Biol. 153, 33–40. doi: 10.1111/j.1744-7348.2008.00235.x

CrossRef Full Text | Google Scholar

Baldissera, T. C., Frak, E., de Faccio Carvalho, P. C., and Louarn, G. (2014). Plant development controls leaf area expansion in alfalfa plants competing for light. Ann. Bot. 113, 145–157. doi: 10.1093/aob/mct251

PubMed Abstract | CrossRef Full Text | Google Scholar

Barillot, R., Chevalier, V., Combes, D., and Louarn, G. (2012). “Variability of pea morphogenesis. An exploratory study based on six pea genotypes with contrasting architectures,” in Fourth International Symposium on Plant Growth Modelling, Simulation, Visualization and Applications (PMA12), eds M. Kang, Y. Dumont, and Y. Guo (Shanghai: IEEE Press), 43–49.

Google Scholar

Barillot, R., Escobar-Gutiérrez, A. J., Fournier, C., Huynh, P., and Combes, D. (2014). Assessing the effects of architectural variations on light partitioning within virtual wheat–pea mixtures. Ann. Bot. 114, 725–737. doi: 10.1093/aob/mcu099

PubMed Abstract | CrossRef Full Text | Google Scholar

Barillot, R., Louarn, G., Escobar-Gutiérrez, A. J., Huynh, P., and Combes, D. (2011). How good is the turbid medium-based approach for accounting for light partitioning in contrasted grass–legume intercropping systems? Ann. Bot. 108, 1013–1024. doi: 10.1093/aob/mcr199

PubMed Abstract | CrossRef Full Text | Google Scholar

Barre, P., Turner, L. B., and Escobar-Gutiérrez, A. J. (2015). Leaf length variation in perennial forage grasses. Agriculture 5, 682–696. doi: 10.3390/agriculture5030682

CrossRef Full Text | Google Scholar

Belaygue, C., Wery, J., Cowan, A., and Tardieu, F. (1996). Contribution of leaf expansion, rate of leaf appearance, and stolon branching to growth of plant leaf area under water deficit in white clover. Crop Sci. 36, 1240–1246. doi: 10.2135/cropsci1996.0011183x003600050028x

CrossRef Full Text | Google Scholar

Beuselinck, P. R., Bouton, J. H., Lamp, W. O., Matches, A. G., McCaslin, M. H., Nelson, C. J., et al. (1994). Improving legume persistence in forage crop systems. J. Prod. Agric. 7, 311–322. doi: 10.2134/jpa1994.0311

CrossRef Full Text | Google Scholar

Black, A. D., Moot, D. J., and Lucas, R. J. (2006). Development and growth characteristics of Caucasian and white clover seedlings, compared with perennial ryegrass. Grass Forage Sci. 61, 442–453. doi: 10.1111/j.1365-2494.2006.00553.x

CrossRef Full Text | Google Scholar

Briske, D. D. (1996). “Strategies of plant survival in grazed systems: a functional interpretation,” in The Ecology and Management of Grazing Systems, eds J. Hodgson and A. W. Illius (Wallingford: CAB International), 37–67.

Google Scholar

Caldwell, M. M. (1987). “Plant architecture and resource competition,” in Potentials and Limitations of Ecosystem Analysis - Ecological Studies, 61, eds E. D. Schulze and H. Zwölfer (Berlin-Heidelberg: Springer-Verlag), 164–179.

Google Scholar

Costes, E., and Guédon, Y. (2002). Modelling branching patterns on 1-year-old trunks of six apple cultivars. Ann. Bot. 89, 513–524. doi: 10.1093/aob/mcf078

PubMed Abstract | CrossRef Full Text | Google Scholar

Davies, A. (2001). “Competition between grasses and legumes in established pastures,” in Competition and Succession in Pastures, eds P. G. Tow and A. Lazenby (Oxon: CABI Publishing), 63–83.

Google Scholar

de Kroons, H., and Hutchings, M. J. (1995). Morphological plasticity in clonal plants: the foraging concept reconsidered. J. Ecol. 83, 143–152. doi: 10.2307/2261158

CrossRef Full Text | Google Scholar

Demotes-Mainard, S., Bertheloot, J., Boumaza, R., Huché-Thélier, L., Guéritaine, G., Guérin, V., et al. (2013). Rose bush leaf and internode expansion dynamics: analysis and development of a model capturing interplant variability. Front. Plant Sci. 4:418. doi: 10.3389/fpls.2013.00418

PubMed Abstract | CrossRef Full Text | Google Scholar

Forde, M. B., Hay, M. J. M., and Brock, J. L. (1989). “Development and growth characteristics of temperate perennial legumes,” in Persistence of Forage Legume, eds G. C. Marten, A. G. Matches, R. F. Barnes, R. W. Brougham, R. J. Clements, and G. W. Sheath (Madison, WI: American Society of Agronomy), 91–110.

Google Scholar

Fort, F., Cruz, P., Catrice, O., Delbrut, A., Luzarreta, M., Stroia, C., et al. (2015). Root functional trait syndromes and plasticity drive the ability of grassland Fabaceae to tolerate water and phosphorus shortage. Environ. Exp. Bot. 110, 62–72. doi: 10.1016/j.envexpbot.2014.09.007

CrossRef Full Text | Google Scholar

Fournier, C., Durand, J. L., Ljutovac, S., Schäufele, R., Gastal, F., and Andrieu, B. (2005). A functional-structural model of elongation of the grass leaf and its relationships with the phyllochron. New Phytol. 166, 881–894. doi: 10.1111/j.1469-8137.2005.01371.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Gaba, S., Lescourret, F., Boudsocq, S., Enjalbert, J., Hinsinger, P., Journet, E. P., Navas, M. L., et al. (2015). Multiple cropping systems as drivers for providing multiple ecosystem services: from concepts to design. Agron. Sustain. Dev. 35, 607–623. doi: 10.1007/s13593-014-0272-z

CrossRef Full Text | Google Scholar

Gastal, F., and Saugier, B. (1986). Nitrogen nutrition and growth of tall fescue. 2. Nitrogen uptake and partitioning among plant organs. Agronomie 6, 363–370. doi: 10.1051/agro:19860406

CrossRef Full Text | Google Scholar

Gautier, H., Měch, R., Prusinkiewicz, P., and Varlet-Grancher, C. (2000). 3D Architectural modelling of aerial photomorphogenesis in white clover (Trifolium repens L.) using L-systems. Ann. Bot. 85, 359–370. doi: 10.1006/anbo.1999.1069.

CrossRef Full Text | Google Scholar

Gautier, H., Varlet-Grancher, C., and Hazard, L. (1999). Tillering responses to the light environment and to defoliation in populations of perennial ryegrass (Lolium perenne L.) selected for contrasting leaf length. Ann. Bot. 83, 423–429. doi: 10.1006/anbo.1998.0840

CrossRef Full Text | Google Scholar

Génard, M., Pagès, L., and Kervella, J. (1994). Relationship between sylleptic branching and components of parent shoot development in the peach tree. Ann. Bot. 74, 465–470. doi: 10.1006/anbo.1994.1142

CrossRef Full Text | Google Scholar

Genrich, K. C., Sheaffer, C. C., and Ehlke, N. J. (1998). Kura clover growth and development during the seeding year. Crop Sci. 38, 735–741. doi: 10.2135/cropsci1998.0011183x003800030020x

CrossRef Full Text | Google Scholar

Gosse, G., Lemaire, G., Chartier, M., and Balfourier, F. (1988). Structure of a lucerne population (Medicago sativa L.) and dynamics of stem competition for light during regrowth. J. Appl. Ecol. 25, 609–617. doi: 10.2307/2403848

CrossRef Full Text | Google Scholar

Granier, C., Massonnet, C., Turc, O., Muller, B., Chenu, K., and Tardieu, F. (2002). Individual leaf development in Arabidopsis thaliana: a stable thermal-time-based programme. Ann. Bot. 89, 595–604. doi: 10.1093/aob/mcf085

PubMed Abstract | CrossRef Full Text | Google Scholar

Jensen, E. S., Peoples, M. B., Boddey, R. M., Gresshoff, P. M., Hauggaard-Nielsen, H., Alves, B. J. R., et al. (2012). Legumes for mitigation of climate change and the provision of feedstock for biofuels and biorefineries. A review. Agron. Sustain. Dev. 32, 329–364. doi: 10.1007/s13593-011-0056-7

CrossRef Full Text | Google Scholar

Kraft, N. J., Godoy, O., and Levine, J. M. (2015). Plant functional traits and the multidimensional nature of species coexistence. Proc. Natl. Acad. Sci. U.S.A. 112, 797–802. doi: 10.1073/pnas.1413650112

PubMed Abstract | CrossRef Full Text | Google Scholar

Lafarge, M., and Durand, J. L. (2013). Comment L'herbe Pousse: Développement Végétatif, Structures Clonales et Spatiales des Graminées. Versailles: Quae Editions.

Lebon, E., Pellegrino, A., Louarn, G., and Lecoeur, J. (2006). Branch development controls leaf area dynamics in grapevine (Vitis vinifera) growing in drying soil. Ann. Bot. 98, 175–185. doi: 10.1093/aob/mcl085

PubMed Abstract | CrossRef Full Text | Google Scholar

Lebon, E., Pellegrino, A., Tardieu, F., and Lecoeur, J. (2004). Shoot development in grapevine (Vitis vinifera) is affected by the modular branching pattern of the stem and intra-and inter-shoot trophic competition. Ann. Bot. 93, 263–274. doi: 10.1093/aob/mch038

PubMed Abstract | CrossRef Full Text | Google Scholar

Lecoeur, J. (2010). “Vegetative development,” in Physiology of the Pea Crop, eds N. Munier-Jolain, V. Biarnès, I. Chaillet, J. Lecoeur, and M. H. Jeuffroy (Boca Raton, FL: CRC Press), 3–43.

Lemaire, G., and Millard, P. (1999). An ecophysiological approach to modelling resource fluxes in competing plants. J. Exp. Bot. 50, 15–28. doi: 10.1093/jxb/50.330.15

CrossRef Full Text | Google Scholar

Lewis, G., Schrire, B., Mackinder, B., and Lock, M. (2005). Legumes of the World. Kew: Royal Botanic Gardens.

Google Scholar

Louarn, G. (2005). Analyse et Modélisation de L'organogenèse et de L'architecture du Rameau de Vigne (Vitis vinifera L.). PhD. thesis Montpellier: ENSA.

Louarn, G., Andrieu, B., and Giauffret, C. (2010). A size-mediated effect can compensate for transient chilling stress affecting maize (Zea mays) leaf extension. New Phytol. 187, 106–118. doi: 10.1111/j.1469-8137.2010.03260.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Louarn, G., Da Silva, D., Godin, C., and Combes, D. (2012). Simple envelope-based reconstruction methods can infer light partitioning among individual plants in sparse and dense herbaceous canopies. Agric. Forest Meteorol. 166, 98–112. doi: 10.1016/j.agrformet.2012.06.014

CrossRef Full Text | Google Scholar

Louarn, G., Guedon, Y., Lecoeur, J., and Lebon, E. (2007). Quantitative analysis of the phenotypic variability of shoot architecture in two grapevine (Vitis vinifera) cultivars. Ann. Bot. 99, 425–437. doi: 10.1093/aob/mcl276

PubMed Abstract | CrossRef Full Text | Google Scholar

LPWG. (2013). Global legume diversity assessment: concepts, key indicators, and strategies. Taxon 62, 249–266. doi: 10.12705/622.12

CrossRef Full Text

Maamouri, A., Louarn, G., Gastal, F., Béguier, V., and Julier, B. (2015). Effects of lucerne genotype on morphology, biomass production and nitrogen content of lucerne and tall fescue in mixed pastures. Crop Past. Sci. 66, 192–204. doi: 10.1071/CP14164

CrossRef Full Text | Google Scholar

Maître, J. P., Assemat, L., and Jacquard, P. (1985). Croissance du trèfle violet (Trifolium pratense L.) en association avec du ray-grass d'Italie (Lolium multiflorum Lam. ssp. italicum). I. – Description de l'organisation morphologique du trèfle. Agronomie 5, 251–60. doi: 10.1051/agro:19850307

CrossRef Full Text | Google Scholar

McSteen, P., and Leyser, O. (2005). Shoot branching. Annu. Rev. Plant Biol. 56, 353–374. doi: 10.1146/annurev.arplant.56.032604.144122

PubMed Abstract | CrossRef Full Text | Google Scholar

Moreau, D., Salon, C., and Munier-Jolain, N. (2006). Using a standard framework for the phenotypic analysis of Medicago Truncatula: an effective method for characterizing the plant material used for functional genomics approaches. Plant Cell Environ. 29, 1087–1098. doi: 10.1111/j.1365-3040.2005.01483.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Moreau, D., Salon, C., and Munier-Jolain, N. (2007). A Model-based framework for the phenotypic characterization of the flowering of Medicago Truncatula. Plant Cell Environ. 30, 213–224. doi: 10.1111/j.1365-3040.2006.01620.x

PubMed Abstract | CrossRef Full Text | Google Scholar

Moulia, B., Edelin, C., Jeuffroy, M. H., Allirand, J. M., Loup, C., and Chartier, M. (2000). “Preliminary analysis of the architecture of cultivated herbaceous plants,” in Fonctionnement des Peuplements Végétaux Sous Contraintes Environnementales, eds P. Maillard and R. Bonhomme (Paris: INRA Editions), 149–184.

Google Scholar

Nakaya, M., Tsukaya, H., Murakami, N., and Kato, M. (2002). Brassinosteroids control the proliferation of leaf cells of Arabidopsis Thaliana. Plant Cell Physiol. 43, 239–244. doi: 10.1093/pcp/pcf024.

PubMed Abstract | CrossRef Full Text | Google Scholar

Nelson, C. J. (2000). “Shoot morphological plasticity of grasses: leaf growth vs. tillering,” in Grassland Ecophysiology and Grazing Ecology, eds G. Lemaire, J. Hodgson, A. D. Moraes, P. D. F. Carvalho, and C. Nabinger (New York, NY: CAB International Publishing), 101–126.

Google Scholar

Nyfeler, D., Huguenin-Elie, O., Suter, M., Frossard, E., and Lüscher, A. (2011). Grass–legume mixtures can yield more nitrogen than legume pure stands due to mutual stimulation of nitrogen uptake from symbiotic and non-symbiotic sources. Agric. Ecosyst. Environ. 140, 155–163. doi: 10.1016/j.agee.2010.11.022

CrossRef Full Text | Google Scholar

Owens, L. B., Edwards, W. M., and Van Keuren, R. W. (1994). Groundwater nitrate levels under fertilized grass and grass-legume pastures. J. Environ. Qual. 23:752. doi: 10.2134/jeq1994.00472425002300040020x

CrossRef Full Text | Google Scholar

Pagès, L. (2014). Branching patterns of root systems: quantitative analysis of the diversity among dicotyledonous species. Ann. Bot. 114, 591–598. doi: 10.1093/aob/mcu145

PubMed Abstract | CrossRef Full Text | Google Scholar

Pellegrino, A., Lebon, E., Simmoneau, T., and Wery, J. (2005). Towards a simple indicator of water stress in grapevine (Vitis vinifera L.) based on the differential sensitivities of vegetative growth components. Aust. J. Grape Wine Res. 11, 306–315. doi: 10.1111/j.1755-0238.2005.tb00030.x

CrossRef Full Text | Google Scholar

Prévot, L., Ariès, F., and Monestiez, P. (1991). Modélisation de la structure géométrique du maïs. Agronomie 11, 491–503. doi: 10.1051/agro:19910606

CrossRef Full Text | Google Scholar

Rameau, C., Bertheloot, J., Leduc, N., Andrieu, B., Foucher, F., and Sakr, S. (2015). Multiple pathways regulate shoot branching. Front. Plant Sci. 5:741. doi: 10.3389/fpls.2014.00741

PubMed Abstract | CrossRef Full Text | Google Scholar

R Development Core Team (2014). R: A Language and Environment for Statistical Computing. Vienna: R Foundation for Statistical Computing.

Ross, J. K. (1981). The Radiation Regime and Architecture of Plant Stands. Hague: Dr. W. Junk.

Google Scholar

Russelle, M. P., Lamb, J. F. S., Montgomery, B. R., Elsenheimer, D. W., Miller, B. S., and Vance, C. P. (2001). Alfalfa rapidly remediates excess inorganic N at a fertilizer spill site. J. Environ. Qual. 30, 30–36. doi: 10.2134/jeq2001.30130x

PubMed Abstract | CrossRef Full Text | Google Scholar

Schwarz, D., and Kläring, H. P. (2001). Allometry to estimate leaf area of tomato. J. Plant Nutr. 24, 1291–1309. doi: 10.1081/pln-100106982

CrossRef Full Text | Google Scholar

Schwinning, S., and Parsons, A. J. (1996). “Interactions between grasses and legumes: understanding variability in species composition,” in Legumes in Sustainable Farming Systems, Occasional Symposium - British Grassland Society No. 30. ed D. Younie (Reading: British Grassland Society), 153–163.

Google Scholar

Scott, D., Hoglund, J. H., Crush, J. R., and Keoghan, J. M. (1989). “Environmental selection of legumes,” in Persistence of Forage Legume, eds G. C. Marten, A. G. Matches, R. F. Barnes, R. W. Brougham, R. J. Clements, and G. W. Sheath (Madison, WI: American Society of Agronomy), 205–214.

Google Scholar

Seleznyova, A. N., Thorp, T. G., Barnett, A. M., and Costes, E. (2002). Quantitative analysis of shoot development and branching patterns in Actinidia. Ann. Bot. 89, 471–482. doi: 10.1093/aob/mcf069

PubMed Abstract | CrossRef Full Text | Google Scholar

Sheaffer, C. C. (1989). “Effect of competition on legume persistence,” in Persistence of Forage Legume, eds G. C. Marten, A. G. Matches, R. F. Barnes, R. W. Brougham, R. J. Clements, and G. W. Sheath (Madison, WI: American Society of Agronomy), 327–333.

Google Scholar

Simon, J. C., and Lemaire, G. (1987). Tillering and leaf area index in grasses in the vegetative phase. Grass Forage Sci. 42, 373–380. doi: 10.1111/j.1365-2494.1987.tb02127.x

CrossRef Full Text | Google Scholar

Skinner, R. H., and Nelson, C. J. (1994). Epidermal cell division and the coordination of leaf and tiller development. Ann. Bot. 74, 9–16. doi: 10.1006/anbo.1994.1088

PubMed Abstract | CrossRef Full Text | Google Scholar

Smith, S. R. Jr., Bouton, J. H., Singh, A., and McCaughey, W. P. (2000). Development and evaluation of grazing-tolerant alfalfa cultivars: A review. Can. J. Plant Sci. 80, 503–512. doi: 10.4141/p99-048

CrossRef Full Text | Google Scholar

Soussana, J. F., Maire, V., Gross, N., Bachelet, B., Pagès, L., Martin, R., et al. (2012). Gemini: a grassland model simulating the role of plant traits for community dynamics and ecosystem functioning. Parameterization and evaluation. Ecol. Model. 231, 134–145. doi: 10.1016/j.ecolmodel.2012.02.002

CrossRef Full Text | Google Scholar

Suter, M., Connolly, J., Finn, J. A., Loges, R., Kirwan, L., Sebastià, M. T., et al. (2015). Nitrogen yield advantage from grass–legume mixtures is robust over a wide range of legume proportions and environmental conditions. Glob. Chang. Biol. 21, 2424–2438. doi: 10.1111/gcb.12880.

PubMed Abstract | CrossRef Full Text | Google Scholar

Tardieu, F., Reymond, M., Muller, B., Granier, C., Simonneau, T., Sadok, W., et al. (2005). Linking physiological and genetic analyses of the control of leaf growth under changing environmental conditions. Crop Past. Sci. 56, 937–946. doi: 10.1071/ar05156

CrossRef Full Text | Google Scholar

Taylor, N. L., and Quesenberry, K. H. (1996). Red Clover Science, Vol. 28. Dordrecht: Springer Science and Business Media.

Google Scholar

Thomas, R. G. (1987). “The structure of the mature plant,” in White Clover, eds M. J. Baker and W. M. Williams (Wallingford: CAB International), 1–29.

Thomas, R. G. (2003). “Comparative growth forms of dryland forage legumes,” in Legumes for Dryland Pastures, Proceedings of a New Zealand Grassland Association Symposium, ed D. J. Moot (Palmerston North: New Zealand Grassland Association), 18–19.

Google Scholar

Thomas, R. G., Li, F. Y., and Hay, M. J. M. (2014). Differential bud activation by a net positive root signal explains branching phenotype in prostrate clonal herbs: a model. J. Exp. Bot. 65, 673–682. doi: 10.1093/jxb/ert427

PubMed Abstract | CrossRef Full Text | Google Scholar

Turc, O., and Lecoeur, J. (1997). Leaf primordium initiation and expanded leaf production are co-ordinated through similar response to air temperature in Pea (Pisum sativum L.). Ann. Bot. 80, 265–273. doi: 10.1006/anbo.1996.0431

CrossRef Full Text | Google Scholar

Valladares, F., and Niinemets, U. (2007). “The architecture of plant crowns: from design rules to light capture and performance,” in Handbook of Functional Plant Ecology, eds F. I. Pugnaire and F. Valladares (Boca Raton, FL: CRC Press), 101–149.

Van Minnebruggen, A., Cnops, G., Saracutu, O., Goormachtig, S., Van Bockstaele, E., Roldán-Ruiz, I., et al. (2014). Processes underlying branching differences in fodder crops. Euphytica 195, 301–313. doi: 10.1007/s10681-013-0997-9

CrossRef Full Text | Google Scholar

Van Minnebruggen, A., Roldán-Ruiz, I., Van Bockstaele, E., Haesaert, G., and Cnops, G. (2015). The relationship between architectural characteristics and regrowth in Trifolium pratense (red clover). Grass Forage Sci. 70, 507–518. doi: 10.1111/gfs.12138

CrossRef Full Text | Google Scholar

Vertès, F., Jeuffroy, M. H., Louarn, G., Voisin, A. S., and Justes, E. (2015). Légumineuses et prairies temporaires: des fournitures d'azote pour les rotations. Fourrages 223, 221–232. Available online at: http://www.afpf-asso.fr/index/action/page/id/33/title/Les-articles/article/2054

Google Scholar

Villani, P. J., and Demason, D. A. (2000). Roles of the Af and Tl genes in pea leaf morphogenesis: shoot ontogeny and leaf development in the heterozygotes. Ann. Bot. 85, 123–135. doi: 10.1006/anbo.1999.1004.

CrossRef Full Text | Google Scholar

White, J. (1979). The plant as a metapopulation. Annu. Rev. Ecol. Syst. 10, 109–145. doi: 10.1146/annurev.es.10.110179.000545

CrossRef Full Text | Google Scholar

Zaka, S., Ahmed, L. Q., Escobar-Gutiérrez, A. J., Gastal, F., Julier, B., and Louarn, G. (2017). How variable are non-linear developmental responses to temperature in two perennial forage species? Agric. Forest Meteorol. 232, 433–442. doi: 10.1016/j.agrformet.2016.10.004

CrossRef Full Text | Google Scholar

Zhu, J., Andrieu, B., Vos, J., van der Werf, W., Fournier, C., and Evers, J. B. (2014). Towards modelling the flexible timing of shoot development: simulation of maize organogenesis based on coordination within and between phytomers. Ann. Bot. 114, 753–762. doi: 10.1093/aob/mcu051

PubMed Abstract | CrossRef Full Text | Google Scholar

Keywords: forage legumes, morphogenesis, branching, architecture, leaf area, growth habit, competitive ability

Citation: Faverjon L, Escobar-Gutiérrez AJ, Litrico I and Louarn G (2017) A Conserved Potential Development Framework Applies to Shoots of Legume Species with Contrasting Morphogenetic Strategies. Front. Plant Sci. 8:405. doi: 10.3389/fpls.2017.00405

Received: 30 November 2016; Accepted: 09 March 2017;
Published: 27 March 2017.

Edited by:

Maria Carlota Vaz Patto, Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Portugal

Reviewed by:

Hao Peng, Washington State University, USA
Gerda Cnops, Institute for Agricultural and Fisheries Research (ILVO), Belgium

Copyright © 2017 Faverjon, Escobar-Gutiérrez, Litrico and Louarn. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

*Correspondence: Gaëtan Louarn, gaetan.louarn@inra.fr