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Front. Plant Sci. | doi: 10.3389/fpls.2017.02043

Arabidopsis TCP transcription factors interact with the SUMO conjugating machinery in nuclear foci

  • 1Swammerdam Institute for Life Sciences (SILS), University of Amsterdam, Netherlands
  • 2C.S. Bond Life Sciences Center and Interdisciplinary Plant Group, University of Missouri, United States

In Arabidopsis more than 400 proteins have been identified as SUMO targets, both in vivo and in vitro. Among others, transcription factors (TFs) are common targets for SUMO conjugation. Here we aimed to exhaustively screen for TFs that interact with the SUMO machinery using an arrayed yeast two-hybrid library containing more than 1,100 TFs. We identified 76 interactors that foremost interact with the SUMO conjugation enzyme SCE1 and/or the SUMO E3 ligase SIZ1. These interactors belong to various TF families, which control a wide range of processes in plant development and stress signaling. Amongst these interactors, the TCP family was overrepresented with several TCPs interacting with different proteins of the SUMO conjugation cycle. For a subset of these TCPs we confirmed that the catalytic site of SCE1 is essential for this interaction. In agreement, TCP1, TCP3, TCP8, TCP14 and TCP15 were readily SUMO modified in an E. coli sumoylation assay. Strikingly, these TCP-SCE1 interactions were found to redistribute these TCPs into nuclear foci/speckles, suggesting that these TCP foci represent sites for SUMO (conjugation) activity.

Keywords: SUMO, TCP, Transcription Factors, SUMO conjugation, yeast two-hybrid

Received: 18 Sep 2017; Accepted: 15 Nov 2017.

Edited by:

Stéphane Bourque, Université de Bourgogne, France

Reviewed by:

Xianzhong Feng, Northeast Institute of Geography and Agroecology (CAS), China
Mingqi Zhou, University of Florida, United States  

Copyright: © 2017 Mazur, Djajasaputra, van der Gragt, Spears, Vlachakis, Beerens, Gassmann and van den Burg. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Harrold A. van den Burg, DR., University of Amsterdam, Swammerdam Institute for Life Sciences (SILS), Science Park 904, Amsterdam, 1098 XH, Netherlands, h.a.vandenburg@uva.nl