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Front. Plant Sci. | doi: 10.3389/fpls.2018.00207

Genome wide identification and expression profiling of SWEET genes family reveals its role during Plasmodiophora brassicae-induced formation of clubroot in Brassica rapa

Hong Li1, Xiaonan Li1,  Yuanhu Xuan2, Jing Jiang1,  Yangdou Wei3 and  Zhongyun Piao1*
  • 1Department of Horticulture, Shenyang Agricultural University, China
  • 2Department of Plant Protection, Shenyang Agricultural University, China
  • 3University of Saskatchewan, Canada

Plasmodiophora brassicae is a soil borne pathogen and the causal agent of clubroot, a devastating disease of Brassica crops. The pathogen lives inside roots, and hijacks nutrients from the host plants. It is suggested that clubroot galls created an additional nutrient sink in infected roots. However, the molecular mechanism underlying P. brassicae infection and sugar transport is unclear. Here, we analyzed sugar contents in leaves and roots before and after P. brassicae infection using a pair of Chinese cabbage near-isogenic lines (NILs), carrying either a clubroot resistant (CR) or susceptible (CS) allele at the CRb locus. P. brassicae infection caused significant increase of glucose and fructose contents in the root of CS-NIL compared to CR-NIL, suggesting that sugar translocation and P. brassicae growth are closely related. Among 32 B. rapa SWEET homologs, several BrSWEETs belonging to Clade I and III were significantly up-regulated, especially in CS-NIL upon P. brassicae infection. Moreover, Arabidopsis sweet11 mutant exhibited slower gall formation compared to the wild-type plants. Our studies suggest that P. brassicae infection probably triggers active sugar translocation between the sugar producing tissues and the clubbed tissues, and the SWEET family genes are involved in this process.

Keywords: Brassica rapa, Sweets, Sugar translocation, Plasmodiophora brassicae, clubroot

Received: 13 Oct 2017; Accepted: 02 Feb 2018.

Edited by:

Mahmut Tör, University of Worcester, United Kingdom

Reviewed by:

Hossein Borhan, Agriculture and Agri-Food Canada (AAFC), Canada
Volkan Cevik, University of Bath, United Kingdom  

Copyright: © 2018 Li, Li, Xuan, Jiang, Wei and Piao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Zhongyun Piao, Shenyang Agricultural University, Department of Horticulture, Shenyang, China,