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Front. Plant Sci. | doi: 10.3389/fpls.2018.00217

Identification of metabolites and transcripts involved in salt stress and recovery in peanut

 Feng Cui1,  Na Sui2,  Guangyou Duan3, Yiyang Liu1, Yan Han1, Shanshan Liu2, Shubo Wan1* and  Guowei Li1*
  • 1Shandong Academy of Agricultural Sciences, China
  • 2Shandong Normal University, China
  • 3Qilu Normal University, China

Peanut (Arachis hypogaea L.) is considered as a moderately salt-sensitive species and thus soil salinity can be a limiting factor for peanut cultivation. To gain insights into peanut plant physiology in response to salt stress and alleviation, we comprehensively characterized leaf relative electrolyte leakage (REC), photosynthesis, leaf transpiration and metabolism of plants under salt stress and plants that were subjected to salt stress followed by salt alleviation period. As expected, we found that REC levels were higher when plants were subjected to salt stress compared with the untreated plants. However, in contrast to expectations, REC was even higher compared with salt treated plants when plants were transferred from salt stress to standard conditions. To decipher REC variation in response to salt stress, especial during the recovery, metabolite and transcript variations were analyzed by GC/MS and RNA-seq method, respectively. Ninety two metabolites, among total 391 metabolites identified, varied in response to salt and 42 metabolites responded to recovery specially. Transcriptomics data showed 1,742 in shoots and 3,281 in roots transcript varied in response to salt stress and 372 in shoots and 1386 transcripts in roots responded specifically to recovery, but not salt stress. Finally, 95 transcripts and 1 metabolite are indicated as candidates involved in REC, photosynthesis, transpiration and Na+ accumulation variation were revealed by using the principal component analysis (PCA) and correlation analysis. This study provides valuable information on peanut response to salt stress and recovery and may inspire further study to improve salt tolerance in peanut germplasm innovation.

Keywords: Metabolomics, peanut, Recovery, salt stress, Transcriptomics

Received: 19 Sep 2017; Accepted: 05 Feb 2018.

Edited by:

Sheng-Hong Li, Kunming Institute of Botany (CAS), China

Reviewed by:

Atsushi Fukushima, RIKEN, Japan
Jianghua Chen, Key Laboratory of Tropical Plant Resources and Sustainable Use, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, China  

Copyright: © 2018 Cui, Sui, Duan, Liu, Han, Liu, Wan and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Shubo Wan, Shandong Academy of Agricultural Sciences, Jinan, China, wansb@saas.ac.cn
Dr. Guowei Li, Shandong Academy of Agricultural Sciences, Jinan, China, ligw@saas.ac.cn