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Front. Plant Sci. | doi: 10.3389/fpls.2018.00240

Transcriptomic Analysis Reveal the Molecular Mechanisms of Wheat Higher-Temperature Seedling-Plant Resistance to Puccinia striiformis f. sp. tritici

  • 1Northwest A&F University, China
  • 2UniqueGene Bioinformatics Science and Technology Co Wuhan, CN, China
  • 3NIAB East Malling Research (EMR) East Malling, UK, East Malling Research (United Kingdom), United Kingdom
  • 4Washington State University, United States

Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a destructive disease of wheat worldwide. The disease is preferably controlled by growing resistant cultivars. Wheat cultivar Xiaoyan 6 (XY 6) has been resistant to stripe rust since its release. In the previous studies, XY 6 was found to have higher-temperature seedling-plant (HTSP) resistance. However, the molecular mechanisms of HTSP resistance were not clear. To identify differentially expressed genes (DEGs) involved in HTSP resistance, we sequenced 30 cDNA libraries constructed from XY 6 seedlings exposed to several temperature treatments. Compared to the constant normal (15°C) and higher (20°C) temperature treatments, 1395 DEGs were identified in seedlings exposed to 20°C for 24 h (to activate HTSP resistance) and then kept at 15°C. These DEGs were located on all 21 chromosomes, with 29.2% on A, 41.1% on B and 29.7% on D genomes, by mapping to the Chinese Spring wheat genome. The 1395 DEGs were enriched in ribosome, plant-pathogen interaction and glycerolipid metabolism pathways, and some of them were identified as hub proteins (phosphatase 2C10), resistance protein homologues, WRKY transcription factors and protein kinases. The majority of these genes were up-regulated in HTSP resistance. Based on the differential expression, we found that phosphatase 2C10 and LRR receptor-like serine/threonine protein kinases are particularly interesting as they may be important for HTSP resistance through interacting with different resistance proteins, leading to a hypersensitive response.

Keywords: high temperature, Non-race-specific resistance, plant defence, Plant-pathogen interaction, Puccinia striiformis f. sp. tritici, Transcript Profiling, wheat

Received: 11 Oct 2017; Accepted: 09 Feb 2018.

Edited by:

Pierre Fobert, National Research Council Canada (NRC-CNRC), Canada

Reviewed by:

André Laroche, Agriculture and Agri-Food Canada (AAFC), Canada
Parveen Chhuneja, Punjab Agricultural University, India  

Copyright: © 2018 Tao, Wang, Guo, Hu, Xu, Chen, Hu and Yang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Xiaoping Hu, Northwest A&F University, Yangling, China, xphu@nwsuaf.edu.cn
Prof. Jiarong Yang, Northwest A&F University, Yangling, China, yljryang@tom.com