Original Research ARTICLE
Transient Expression of CRISPR/Cas9 Machinery Targeting TcNPR3 Enhances Defense Response in Theobroma cacao
- 1Plant Sciences, Pennsylvania State University, United States
- 2The Huck Institutes of the Life Sciences, Pennsylvania State University, United States
Theobroma cacao, the source of cocoa, suffers significant losses to a variety of pathogens resulting in reduced incomes for millions of farmers in developing countries. Development of disease resistant cacao varieties is an essential strategy to combat this threat, but is limited by sources of genetic resistance and the slow generation time of this tropical tree crop. In this study, we present the first application of genome editing technology in cacao, using Agrobacterium-mediated transient transformation to introduce CRISPR/Cas9 components into cacao leaves and cotyledon cells. As a first proof of concept, we targeted the cacao Non-Expressor of Pathogenesis-Related 3 (TcNPR3) gene, a suppressor of the defense response. After demonstrating activity of designed single-guide RNAs (sgRNA) in vitro, we used Agrobacterium to introduce a CRISPR/Cas9 system into leaf tissue, and identified the presence of deletions in ~30% of TcNPR3 copies in the treated tissues. The edited tissue exhibited an increased resistance to infection with the cacao pathogen Phytophthora tropicalis and elevated expression of downstream defense genes. Analysis of off-target mutagenesis in sequences similar to sgRNA target sites using high-throughput sequencing did not reveal mutations above background sequencing error rates. These results confirm the function of NPR3 as a repressor of the cacao immune system and demonstrate the application of CRISPR/Cas9 as a powerful functional genomics tool for cacao. Several stably transformed and genome edited somatic embryos were obtained via Agrobacterium-mediated transformation, and ongoing work will test the effectiveness of this approach at a whole plant level.
Keywords: NPR3, theobroma cacao, gene editing, CRISPR/Cas9, plant defense response, Transient transformation
Received: 08 Nov 2017;
Accepted: 14 Feb 2018.
Edited by:Marcelo M. Menossi, Universidade Estadual de Campinas, Brazil
Reviewed by:Kaijun Zhao, Institute of Crop Science, Chinese Academy of Agricultural Sciences, China
Kan Wang, Iowa State University, United States
Antonio Figueira, University of São Paulo, Brazil
Copyright: © 2018 Fister, Landherr, Maximova and Guiltinan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: PhD. Mark J. Guiltinan, Pennsylvania State University, Plant Sciences, 418 Life Sciences Building, University Park, 16802, PA, United States, firstname.lastname@example.org