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Front. Plant Sci. | doi: 10.3389/fpls.2018.01279

Reactive oxygen species and gibberellin acid mutual induction to regulate tobacco seed germination

 LI ZHAN1,  Yue Gao1, Yuchan Zhang1, Cheng Lin1,  Dongting Gong1,  Yajing Guan1* and Jin Hu1
  • 1Zhejiang University, China

Seed germination is a complex process controlled by many mechanisms. To examine the potential contribution of reactive oxygen species (ROS) and gibberellin acid (GA) in regulating seed germination, diphenylene iodonium chloride (DPI) and uniconazole (Uni), as hydrogen peroxide (H2O2) and GA synthesis inhibitor respectively, were exogenous applied on tobacco seeds by seed priming. Seed priming with DPI or Uni decreased germination percentages as compared with priming with H2O, especially DPI+Uni combination. H2O2 and GA completely reversed the inhibition induced by DPI or Uni. The germination percentage of H2O2+Uni and GA+DPI combination kept the same level as H2O. Meanwhile, GA or H2O2 increased GA content and deceased ABA content through corresponding genes expression involving in homeostasis and signal transduction. In addition, the activation of storage reserves mobilization, enhancement of soluble sugar content and isocitrate lyase (ICL) activity, was also induced by GA or H2O2. These results strongly suggested that H2O2 and GA were essential for tobacco seed germination and there was a mutual induction between them to regulating seed germination. Meanwhile ICL activity was jointly mediated by ABA/GA balance and ROS concentration.

Keywords: Tobacco seed, Germination, H2O2, GA, ABA, Homeostasis, Signal Transduction

Received: 20 Apr 2018; Accepted: 15 Aug 2018.

Edited by:

Francisco J. Cejudo, Universidad de Sevilla, Spain

Reviewed by:

Sara Maldonado, Universidad de Buenos Aires, Argentina
Francisca Sevilla, Centro de Edafología y Biología Aplicada del Segura (CEBAS), Spain  

Copyright: © 2018 ZHAN, Gao, Zhang, Lin, Gong, Guan and Hu. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Yajing Guan, Zhejiang University, Hangzhou, China, vcguan@zju.edu.cn