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Front. Plant Sci. | doi: 10.3389/fpls.2018.01681

Proteomic analysis of plasmodesmata from Populus cell suspension cultures in relation with callose biosynthesis

  • 1Royal Institute of Technology, Sweden
  • 2Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Germany
  • 3University of South Australia, Australia

Plasmodesmata are channels that link adjacent cells in plant tissues through which molecular exchanges take place. They are involved in multiple processes vital to plant cells, such as responses to hormonal signalling or environmental challenges including osmotic stress, wounding and pathogen attack. Despite the importance of plasmodesmata, their proteome is not well defined. Here we have isolated fractions enriched in plasmodesmata from cell suspension cultures of Populus trichocarpa and identified 201 proteins that are enriched in these fractions, thereby providing further insight on the multiple functions of plasmodesmata. Proteomics analysis revealed an enrichment of proteins specifically involved in responses to stress, transport, metabolism and signal transcription. Consistent with the role of callose deposition and turnover in the closure and aperture of the plasmodesmata and our proteomic analysis, we demonstrate the enrichment of callose synthase activity in the plasmodesmata represented by several gene products. A new form of calcium-independent callose synthase activity was detected, in addition to the typical calcium-dependent enzyme activity, suggesting a role of calcium in the regulation of plasmodesmata through two forms of callose synthase activities. Our report provides the first proteomic investigation of the plasmodesmata from a tree species and the direct biochemical evidence for the occurrence of several forms of active callose synthases in these structures.

Keywords: Populus, Plasmodesmata, callose synthase, callose, Mass Spectrometry, spectral counting

Received: 24 Jul 2018; Accepted: 29 Oct 2018.

Edited by:

Norbert ROLLAND, Centre national de la recherche scientifique (CNRS), France

Reviewed by:

YOSELIN BENITEZ-ALFONSO, University of Leeds, United Kingdom
Benedetta Mattei, University of L'Aquila, Italy
Laigeng Li, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences (CAS), China  

Copyright: © 2018 Leijon, Melzer, Zhou, Srivastava and Bulone. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Dr. Vaibhav Srivastava, Royal Institute of Technology, Stockholm, Sweden,
Prof. Vincent Bulone, Royal Institute of Technology, Stockholm, Sweden,