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Frontiers in Fungal Virus Research

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Front. Plant Sci. | doi: 10.3389/fpls.2018.01699

Identification of a Novel Partitivirus of Trichoderma harzianum NFCF319 and Evidence for the Related Antifungal Activity

  • 1Chonbuk National University, South Korea

We have reported 15 agarose gel band patterns of double-stranded RNA (dsRNA) from Trichoderma spp.. We describe herein that band pattern IX in Trichoderma harzianum NFCF319, which appeared to be a single band but consisted of two dsRNAs of similar size, was identified as a novel mycovirus, designated Trichoderma harzianum partitivirus 1 (ThPV1). The larger segment (dsRNA1) of the ThPV1 genome comprised 2,289 bp and contained a single open reading frame (ORF) encoding a RNA-dependent RNA polymerase (RdRp). The smaller segment (dsRNA2) consisted of 2,245 bp with a single ORF encoding capsid protein (CP). Evaluation of the deduced amino acid sequence and phylogenetic analysis indicated that ThPV1 is a new member of the genus Betapartitivirus in the family Partitiviridae. Curing of virus infection by single-sporing generated 31 virus-free single-spore clones. No significant differences in growth rate, conidia production, or pigmentation were observed between ThPV1-infected and -cured isogenic strains. In addition, comparison of the newly ThPV1-transmitted isolates with their ThPV1-cured parental strain showed no significant difference in colony morphology or pigmentation. However, inhibition of growth in co-cultured Pleurotus ostreatus and Rhizoctonia solani by T. harzianum was increased in ThPV1-containing strains compared with ThPV1-cured isogenic strains. Moreover, -1,3-glucanase activity was significantly increased in the ThPV1-containing strains. However, no difference in chitinase activity was observed, suggesting that ThPV1 regulates the activity of a specific fungal enzyme.

Keywords: Trichoderma harzianum, Mycovirus, Partitivirus, Mycoparasitism, Antifungal activity

Received: 29 Jul 2018; Accepted: 31 Oct 2018.

Edited by:

Massimo Turina, Italian National Research Council, Italy

Reviewed by:

Sotaro Chiba, Asian Satellite Campuses Institute, Nagoya University, Japan
Jarkko Hantula, Natural Resources Institute Finland (Luke), Finland  

Copyright: © 2018 Chun, Yang and Kim. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Dae-Hyuk Kim, Chonbuk National University, Jeonju, South Korea, dhkim@jbnu.ac.kr