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Front. Plant Sci. | doi: 10.3389/fpls.2018.01721

Role for Arabidopsis PLC7 in stomatal movement, seed mucilage attachment, and leaf serration

  • 1Swammerdam Institute for Life Sciences, University of Amsterdam, Netherlands
  • 2Wageningen University & Research, Netherlands
  • 3National University of Mar del Plata, Argentina
  • 4Swammerdam Institute for Life Sciences, section Plant Cell Biology, University of Amsterdam, Netherlands

Phospholipase C (PLC) has been suggested to play important roles in plant stress and development. To increase our understanding of PLC signaling in plants, we have started to analyze knock-out (KO), knock-down (KD) and overexpression mutants of Arabidopsis thaliana, which contains nine PLCs. Earlier, we characterized PLC2, PLC3 and PLC5. Here, the role of PLC7 is functionally addressed. Promoter-GUS analyses revealed that PLC7 is specifically expressed in the phloem of roots, leaves and flowers, and is also present in trichomes and hydathodes. Two T-DNA insertion mutants were obtained, i.e. plc7-3 being a KO- and plc7-4 a KD line. In contrast to earlier characterized phloem-expressed PLC mutants, i.e. plc3 and plc5, no defects in primary- or lateral root development were found for plc7 mutants. Like plc3 mutants, they were less sensitive to ABA during stomatal closure. Double-knockout plc3 plc7 lines were lethal, but plc5 plc7 (plc5/7) double mutants were viable, and revealed several new phenotypes, not observed earlier in the single mutants. These include a defect in seed mucilage, enhanced leaf serration, and an increased tolerance to drought. Overexpression of PLC7 enhanced drought tolerance too, similar to what was earlier found for PLC3-and PLC5 overexpression. In vivo 32Pi-labeling of seedlings and treatment with sorbitol to mimic drought stress, revealed stronger PIP2 responses in both drought-tolerant plc5/7 and PLC7-OE mutants. Together, these results show novel functions for PLC in plant stress and development. Potential molecular mechanisms are discussed.

Keywords: PLC, phospholipase C, Seed mucilage, leaf serration, ABA sensitivity, drought tolerance

Received: 24 Sep 2018; Accepted: 05 Nov 2018.

Edited by:

Yvon Jaillais, École Normale Supérieure de Lyon, France

Reviewed by:

Henri Batoko, Université Catholique de Louvain, Belgium
Catalin Voiniciuc, Heinrich Heine Universität Düsseldorf, Germany
Eric Ruelland, Centre national de la recherche scientifique (CNRS), France  

Copyright: © 2018 Zhang, Wijk, Zarza, Lamers, Reyes-Marquez, Guardia, Scuffi, García-Mata, Ligterink, Haring, Laxalt and Munnik. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Mr. Ringo v. Wijk, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, 1090 GE Amsterdam, Netherlands, R.vanWijk@uva.nl
Prof. Teun Munnik, University of Amsterdam, Swammerdam Institute for Life Sciences, section Plant Cell Biology, Amsterdam, 1098 XH, Netherlands, T.Munnik@uva.nl