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Front. Plant Sci. | doi: 10.3389/fpls.2019.00031

Progress in somatic embryogenesis of Japanese pines

  • 1Forestry and Forest Products Research Institute, Japan

Somatic embryogenesis (SE) in not only one of the most promising techniques for mass propagation of selected trees, but also is a valuable tool for basic research studies in cell biology and genetic engineering, and it allows the long-term ex situ conservation of genetic resources by cryopreservation techniques. This review reports the most recent progress in SE, protoplast culture, and cryopreservation of four important Japanese pines (Pinus thunbergii, P. densiflora, P. armandii var. amamiana, and P. luchuensis). SE initiation, embryogenic culture maintenance/proliferation, production of somatic embryos, germination, and conversion to plants are described focusing on the protocols most commonly reported for plant production in Pinus species through to SE.

Keywords: Clonal propagation, Cryopreservation, Embryogenic cultures, Gellan gum, liquid culture, polyethylene glycol, Protoplasts, somatic embryo desiccation

Received: 23 Jul 2018; Accepted: 09 Jan 2019.

Edited by:

Jorge M. Canhoto, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, Portugal

Reviewed by:

Matthew R. Willmann, Cornell University, United States
Atanas I. Atanassov, Joint Genomic Center, Bulgaria  

Copyright: © 2019 Maruyama and Hosoi. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Tsuyoshi E. Maruyama, Forestry and Forest Products Research Institute, Tsukuba, Japan, tsumaruy@ffpri.affrc.go.jp