Original Research ARTICLE
Development of stable homozygous wheat/Amblyopyrum muticum (Aegilops mutica) introgression lines and their cytogenetic and molecular characterisation
- 1BBSRC Wheat Research Centre, University of Nottingham, United Kingdom
- 2Limagrain (United Kingdom), United Kingdom
Wheat is one of the world’s most important sources of food. However, due to its evolution it has a low genetic base which will severely limit the ability of breeders to develop new superior higher yielding varieties that are adapted to the changing environment. In contrast to wheat, its wild relatives provide a vast reserve of genetic variation for most, if not all, agronomically important traits.
Genetic variation has previously been transferred to wheat from one of its wild relatives, Ambylopyrum muticum (previous known as Aegilops mutica). However, before the genetic variation available in this species can be assessed and exploited in breeding and for research, the transmission of the chromosome segments introgressed into wheat carrying it must first be stabilised. In this paper we describe the generation of 69 stably inherited homozygous wheat/Am. muticum introgression lines using a doubled haploid procedure. The characterisation and stability of each of these lines was determined via genomic in situ hybridisation and SNP analysis. The strategy that we are employing for the distribution and exploitation of the genetic variation from Am. muticum and a range of other species is discussed.
Keywords: Amblyopyrum muticum, Introgression, wheat, Genomic in situ hybridisation, Doubled haploid, KASP markers
Received: 17 Sep 2018;
Accepted: 10 Jan 2019.
Edited by:István Molnár, Centre for Agricultural Research (MTA), Hungary
Reviewed by:Mahmoud Said, Institute of Experimental Botany (ASCR), Czechia
Adoracion Cabrera, Universidad de Córdoba, Spain
Copyright: © 2019 King, Newell, Grewal, Edwards, Yang, Scholefield, Ashling, Stride and King. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
* Correspondence: Prof. Ian P. King, BBSRC Wheat Research Centre, University of Nottingham, Sutton Bonington, United Kingdom, firstname.lastname@example.org