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Front. Plant Sci. | doi: 10.3389/fpls.2019.00217

Heterologous expression of serine hydroxymethyltransferase3 from rice confers tolerance to salinity stress in E coli and Arabidopsis

  • 1National Research Centre on Plant Biotechnology (ICAR), India
  • 2Amity University Jaipur, India
  • 3Meijo University, Japan
  • 4Central Soil Salinity Research Institute (ICAR), India

Among abiotic stresses, salinity stress adversely affects growth and development in rice. Contrasting salt tolerant (CSR27), and salt sensitive (MI48) rice varieties provided information on an array of genes that may contribute for salt tolerance of rice. Earlier studies on transcriptome and proteome profiling led to the identification of salinity stress-induced serine hydroxyl methyltransferase-3 (SHMT3) gene. In the present study, SHMT3 gene was isolated from salt-tolerant (CSR27) rice. The functional characterization of OsSHMT3 from salt-tolerant OsSHMT3 exhibited salinity-stress induced accentuated and differential expression levels in different tissues of rice. OsSHMT3 was overexpressed in E. coli and assayed for enzymatic activity and modelling protein structure. Further, Arabidopsis transgenic plants overexpressing OsSHMT3 exhibited tolerance towards salinity stress. Comparative analyses of OsSHMT3 vis a vis wild type by ionomic, transcriptomic and metabolic profiling, protein expression and analysis of various agronomic traits revealed a pivotal role of OsSHMT3 in conferring tolerance towards salinity stress. The gene can further be used in developing gene based markers for salinity stress to be employed in marker assisted breeding programs.

Keywords: rice,, Salinity, Serine, Glycine, aquaporin

Received: 26 Jun 2018; Accepted: 08 Feb 2019.

Edited by:

Sonia Negrao, University College Dublin, Ireland

Reviewed by:

Ahmad Arzani, Isfahan University of Technology, Iran
Andrew Wood, Southern Illinois University Carbondale, United States
Suriyan Cha-um, National Science and Technology Development Agency (NSTDA), Thailand  

Copyright: © 2019 Mishra, JAIN, Takabe, Tanaka, NEGI, Singh, Jain, MISHRA, Maniraj, S. L., Sreevathsa, Singh and Rai. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Vandna Rai, National Research Centre on Plant Biotechnology (ICAR), New Delhi, Delhi, India, vandnarai2006@gmail.com