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Front. Plant Sci. | doi: 10.3389/fpls.2019.00548

Chromosome Arm Locations of Barley Sucrose Transporter Gene in Transgenic Winter Wheat Lines

  • 1Faculty of Agriculture, Ryukoku University, Japan
  • 2Department of Molecular Genetics, Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Germany
  • 3Department of Breeding Research, Abteilung Molekulare Genetik, Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Germany
  • 4Institute of Plant Science and Resources, Okayama University, Japan

Three transgenic HOSUT lines of winter wheat, HOSUT12, HOSUT20, and HOSUT24, each harbor a single copy of the cDNA for the barley sucrose transporter gene HvSUT1 (SUT), which was fused to the barley endosperm-specific Hordein B1 promotor (HO) (the HOSUT transgene). Previously, flow cytometry combined with PCR analysis demonstrated that the HOSUT transgene had been integrated into different wheat chromosomes: 7A, 5D and 4A in HOSUT12, HOSUT20, and HOSUT24, respectively. In order to confirm the chromosomal location of the HOSUT transgene by a cytological approach using wheat aneuploid stocks, we crossed corresponding nullisomic-tetrasomic lines with the three HOSUT lines, namely nullisomic 7A-tetrasomic 7B with HOSUT12, nullisomic 5D-tetrasomic 5B with HOSUT20, and nullisomic 4A-tetrasomic 4B with HOSUT24. We examined the resulting chromosomal constitutions and the presence of the HOSUT transgene in the F2 progeny by means of chromosome banding and PCR. The chromosome banding patterns of the critical chromosomes in the original HOSUT lines showed no difference from those of the corresponding wild type chromosomes. The presence or absence of the critical chromosomes completely corresponded to the presence or absence of the HOSUT transgene in the F2 plants. Investigating telocentric chromosomes occurred in the F2 progeny, which were derived from the respective critical HOSUT chromosomes, we found that the HOSUT transgene was individually integrated on the long arms of chromosomes 4A, 7A, and 5D in the three HOSUT lines. Thus, in this study we verified the chromosomal locations of the transgene, which had previously been determined by flow cytometry, and moreover revealed the chromosome-arm locations of the HOSUT transgene in the HOSUT lines.

Keywords: barley sucrose transporter, transgene, HOSUT lines, Chromosome Banding, Telocentric chromosome, Nullisomic-tetrasomics

Received: 30 May 2018; Accepted: 10 Apr 2019.

Edited by:

Isabelle Colas, James Hutton Institute, United Kingdom

Reviewed by:

Andreas Houben, Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Germany
Fangpu Han, State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology (CAS), China  

Copyright: © 2019 Takenaka, Weschke, Brückner, Murata and Endo. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Takashi R. Endo, Ryukoku University, Faculty of Agriculture, Otsu, 520-2194, Shiga, Japan, endo.takashi.2e@agr.ryukoku.ac.jp