Impact Factor 3.677
2017 JCR, Clarivate Analytics 2018

The world's most-cited Plant Sciences journal

This article is part of the Research Topic

Proceedings of ISPMF 2018 - Plant Molecular Farming

Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.00735

Production of biopharmaceuticals in Nicotiana benthamiana – Axillary stem growth as a key determinant of total protein yield

 Marie-Claire Goulet1, Linda Gaudreau1, Marielle Gagné1, Anne-Marie Maltais1,  Ann-Catherine Laliberté1, Gilbert Éthier1,  Nicole Bechtold2,  Michèle Martel2,  Marc-André D'Aoust2, André Gosselin1,  Steeve Pepin1* and  Dominique Michaud1*
  • 1Laval University, Canada
  • 2Medicago (Canada), Canada

Data are scarce about the influence of basic cultural conditions on growth patterns and overall performance of plants used as heterologous production hosts for protein pharmaceuticals. Higher plants are complex organisms with young, mature and senescing organs that show distinct metabolic backgrounds and differ in their ability to sustain foreign protein expression and accumulation. Here, we used the transient protein expression host Nicotiana benthamiana as a model to map the accumulation profile of influenza virus hemagglutinin H1, a clinically promising vaccine antigen, at the whole plant scale. Greenhouse-grown plants submitted to different light regimes, submitted to apical bud pruning or treated with the axillary growth-promoting cytokinin 6-benzylaminopurine were vacuum-infiltrated with agrobacteria harboring a DNA sequence for H1 and allowed to express the viral antigen for 7 d in growth chamber under similar environmental conditions. Our data highlight the importance of young leaves on H1 yield per plant, unlike older leaves which account for a significant part of the plant biomass but contribute little to total antigen titer. Our data also highlight the key contribution of axillary stem leaves, which contribute more than 50% of total yield under certain conditions despite representing only one third of the total biomass. These findings underline the relevance of both considering main stem leaves and axillary stem leaves while modelling heterologous protein production in N. benthamiana. They also demonstrate the potential of exogenous applied growth-promoting hormones to modulate host plant architecture for improvement of protein yields.

Keywords: plant molecular farming, Nicotiana benthamiana, Influenza virus hemagglutinin, Supplemental lighting, Apical pruning, 6-Benzyl aminopurine (6-BA)

Received: 20 Feb 2019; Accepted: 16 May 2019.

Edited by:

Kirsi-Marja Oksman-Caldentey, VTT Technical Research Centre of Finland Ltd, Finland

Reviewed by:

Emmanuel A. Margolin, University of Cape Town, South Africa
Teresa Capell, Universitat de Lleida, Spain  

Copyright: © 2019 Goulet, Gaudreau, Gagné, Maltais, Laliberté, Éthier, Bechtold, Martel, D'Aoust, Gosselin, Pepin and Michaud. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Steeve Pepin, Laval University, Quebec, G1V 0A6, Quebec, Canada,
Prof. Dominique Michaud, Laval University, Quebec, G1V 0A6, Quebec, Canada,