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Front. Plant Sci. | doi: 10.3389/fpls.2019.00741

Compatible and incompatible pollen-styles interaction in Pyrus communis L. show different transglutaminase features, polyamine pattern and metabolomics profiles

  • 1Department of Pharmacy and Biotechnology, University of Bologna, Italy
  • 2Department for Life Quality Studies, University of Bologna, Italy
  • 3Department of Biological Sciences, Geological and Environmental, University of Bologna, Italy
  • 4Department of Life Sciences, University of Siena, Italy
  • 5University of Bologna, Italy

Pollen-stigma interaction is a highly selective process, which leads to compatible or incompatible pollination, in the latter case, affecting quantitative and qualitative aspects of productivity in species of agronomic interest. While the genes and the corresponding protein partners involved in this highly specific pollen-stigma recognition have been studied, providing important insights into pollen-stigma recognition in self-incompatible (SI), many other factors involved in the SI response are not understood yet. This work concerns the study of transglutaminase (TGase), polyamine pattern and metabolomic profiles following the pollination of Pyrus communis L. pistils with compatible and SI pollen in order to deepen their possible involvement in the reproduction of plants. Immunolocalization, abundance and activity of TGase as well as and the content of free, soluble-conjugated and insoluble-bound polyamines have been investigated. 1H NMR-profiling coupled with multivariate data treatment (PCA and PLS-DA) allowed to compare, for the first time, the metabolic patterns of non-pollinated and pollinated styles. Results clearly indicate that during the SI response TGase activity increases, resulting in the accumulation of polyamines conjugated to hydroxycinnamic acids and other small molecules. Metabolomic analysis showed a remarkable differences between pollinated and non-pollinated styles, where, except for glucose, all the other metabolites where less concentrated. Moreover, styles pollinated with compatible pollen showed the highest amount of sucrose than SI pollinated ones, which, in turn, contained highest amount all the other metabolites, including aromatic compounds, such as flavonoids and a cynnamoil derivative.

Keywords: self-incompatibility, Pyrus communis (L), Transglutaminase, 1H NMR-Metabolomics, Polyamines

Received: 15 Mar 2019; Accepted: 17 May 2019.

Edited by:

Antonio F. Tiburcio, University of Barcelona, Spain

Reviewed by:

Penglin Sun, University of California, Riverside, United States
Milagros B. Gonzalez, Universidad de Jaén, Spain  

Copyright: © 2019 Mandrone, ANTOGNONI, Aloisi, Potente, Poli, Cai, Faleri, Parrotta and Del Duca. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Stefano Del Duca, University of Bologna, Bologna, Italy, stefano.delduca@unibo.it