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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01031

Dynamic changes in genome-wide histone3 lysine27 trimethylation and gene expression of soybean roots in response to salt stress

 Lei Sun1, 2,  Guangshu Song3, Weijun Guo4,  Weixuan Wang4,  Hongkun Zhao2, Tingting Gao3, Qingxue Lv3, Xue Yang3, Fan Xu4, Yingshan Dong1, 2 and  LI PU4*
  • 1College of Agriculture, Northeast Agricultural University, China
  • 2Soybean Research Institute, Jilin Academy of Agricultural Sciences, China
  • 3Maize Research Institute, Jilin Academy of Agricultural Sciences, China
  • 4Biotechnology Research Institute (CAAS), China

Soybean is an important economic crop for human diet, animal feeds and biodiesel due to high protein and oil content. Its productivity is significantly hampered by salt stress, which impairs plant growth and development by affecting gene expression, in part, through epigenetic modification of chromatin status. However, little is known about epigenetic regulation of stress response in soybean roots. Here, we used RNA-seq and ChIP-Seq technologies to study the dynamics of genome-wide transcription and histone methylation patterns in soybean roots under salt stress. 8798 soybean genes changed their expression under salt stress treatment. Whole-genome ChIP-seq study of an epigenetic repressive mark, histone H3 lysine 27 trimethylation (H3K27me3), revealed the changes in H3K27me3 deposition during the response to salt stress. Unexpectedly, we found that most of the inactivation of genes under salt stress is strongly correlated with the de novo establishment of H3K27me3 in various parts of the promoter or coding regions where there is no H3K27me3 in control plants. In addition, the soybean histone modifiers were identified which may contribute to de novo histone methylation and gene silencing under salt stress. Thus, dynamic chromatin regulation, switch between active and inactive modes, occur at target loci in order to respond to salt stress in soybean. Our analysis demonstrates histone methylation modifications are correlated with the activation or inactivation of salt-inducible genes in soybean roots.

Keywords: salt stress, RNA-Seq, ChIP-seq, Histone Methylation, Histone modifiers

Received: 21 Mar 2019; Accepted: 23 Jul 2019.

Copyright: © 2019 Sun, Song, Guo, Wang, Zhao, Gao, Lv, Yang, Xu, Dong and PU. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. LI PU, Biotechnology Research Institute (CAAS), Beijing, Beijing Municipality, China, puli@caas.cn