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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01081

Mutation at different sites of metal transporter gene OsNramp5 affects Cd accumulation and related agronomic traits in rice (Oryza sativa L.)

 Tiankang Wang1, 2, Yixing Li2, Yuefeng Fu3, Hongjun Xie4, Shufeng Song2, Mudan Qiu2, Jiong Wen3, Muwen Chen3, Ge Chen3, Yan Tian2, Chengxia Li2, Dingyang Yuan2, Jianlong Wang1* and  Li Li2*
  • 1College of Agriculture, Hunan Agricultural University, China
  • 2State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, China
  • 3Other, China
  • 4Hunan Hybrid Rice Research, China

OsNramp5 is a key gene involved in the control of the uptake of Cd, Mn, and other metal ions by rice root cells. The functional deficiency of this gene can significantly reduce the accumulation of Cd in rice grains, but the effects of its mutation on agronomic traits such as yield and quality have not been investigated comprehensively yet. In the present study, three Huanghuazhan-based OsNramp5 mutants[LCH1(Low Cadmium Huanghuazhan 1), LCH2(Low Cadmium Huanghuazhan 2), and LCH3(Low Cadmium Huanghuazhan 3) ]were obtained using CRISPR/Cas9(Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated Proteins9) technology. The mutation type analysis showed that LCH1, LCH2, and LCH3 encoded defective OsNramp5 protein sequences containing only 76aa, 176aa, or 266aa, respectively. The determination of metal content and the statistics of related agronomic traits revealed that the functional deficiency OsNramp5 not only significantly reduced the accumulation of Cd in the grains of the mutants, but also affected rice yield and quality. However, with the decrease of OsNramp5 mutation degree, its effects on chlorenchyma Mn accumulation, yield and quality were also diminished. Additionally, we also found that the increase in the concentration of Mn in the soil restored the phenotype of the declined yield and quality due to the functional deficiency of OsNramp5. Our findings provide novel insights into and new materials for breeding rice varieties with low Cd accumulation and excellent agronomic traits under severe Cd pollution environment.

Keywords: quality, yield, Cd, rice, OsNramp5

Received: 22 May 2019; Accepted: 08 Aug 2019.

Copyright: © 2019 Wang, Li, Fu, Xie, Song, Qiu, Wen, Chen, Chen, Tian, Li, Yuan, Wang and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Prof. Jianlong Wang, College of Agriculture, Hunan Agricultural University, Changsha, China, 34482548@qq.com
Prof. Li Li, State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, 410125, Hunan Province, China, lili@hhrrc.ac.cn