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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01088

Overexpression of OsARD1 improves submergence, drought and salt tolerance of seedling through the enhancement of ethylene synthesis in rice

 Shanshan Liang1, Wei Xiong1,  Cuicui Yin2, Xiaodong Xie3, Ya-jun Jin4, Siju Zhang4, Bo Yang4,  Guoyou Ye5, Shouyi Chen2 and  Weijiang Luan1*
  • 1College of Life Sciences, Tianjin Normal University, China
  • 2State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology (CAS), China
  • 3Tianjin Agricultural University, China
  • 4Tianjin Normal University, China
  • 5International Rice Research Institute, Philippines

Acireductone dioxygenase (ARD) is a metal binding metalloenzyme and involved in the methionine salvage pathway. In rice, OsARD1 binds Fe2+ and catalyzes the formation of 2-Keto-4-methylthiobutyrate (KMTB) to produce methionine, which is an initial substrate in ethylene synthesis pathway. Here, we report that overexpression of OsARD1 elevates the endogenous ethylene release rate, enhances the tolerance to submergence stress and reduces the sensitivity to drought, salt and osmotic stress in rice. OsARD1 is strongly induced by submergence, drought, salinity, PEG6000, and mechanical damage stresses, and exhibits high expression in senescent leaves. Transgenic plants overexpressing OsARD1 (OsARD1-OE) display fast elongation growth to escape the submergence stress. The ethylene content is significantly maximized in OsARD1-OE plants compared with the wide type. OsARD1-OE plants display increased shoot elongation and inhibition of root elongation under the submergence stress and grow in dark due to increase of ethylene. The elongation of coleoptile under anaerobic germination is also significantly promoted in OsARD1-OE lines due to increase of ethylene. The sensitivity to drought and salt is reduced in OsARD1-OE transgenic lines. Water holding capacity is enhanced and the stomata and trichomes on leaves increase in OsARD1-OE lines to contribute the reduction of the sensitivity to abiotic stresses. Drought and salt tolerance and ethylene synthesis related genes are upregulated in OsARD1-OE plants. Subcellular localization shows that OsARD1 displays strong localization signal in cell nucleus, suggesting OsARD1 may interact with the transcription factors. Taken together, the results provide the understanding of the function of OsARD1 in ethylene synthesis and abiotic stress response in rice.

Keywords: OsARD1, ethylene, rice, submergence tolerance, drought tolerance, Salt tolerace

Received: 31 Jan 2019; Accepted: 09 Aug 2019.

Copyright: © 2019 Liang, Xiong, Yin, Xie, Jin, Zhang, Yang, Ye, Chen and Luan. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Weijiang Luan, College of Life Sciences, Tianjin Normal University, Tianjin, 300387, China,