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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01098

Identification of candidate adaxial–abaxial related genes regulating petal expansion during flower opening in Rosa chinensis ‘Old Blush’

 Yu Han1, Xue Yong1, Jiayao Yu1,  Tangren Cheng1, Jia Wang1, Weiru Yang1, Huitang Pan1 and  Qixiang Zhang1*
  • 1Beijing Forestry University, China

Petal expansion is the main process by which flower opening occurs in roses (Rosa chinensis). While the regulation of leaf expansion has been extensively studied, little is known about the mechanisms controlling petal expansion. The regulation of leaf dorsoventral (adaxial–abaxial) polarity is important for blade expansion and morphogenesis, but the mechanisms involved adaxial-abaxial regulation in petals are unknown. We found that auxin, a key hormonal regulator of leaf adaxial–abaxial patterning, is unevenly distributed in rose petals. The transcriptomes of the adaxial and abaxial petal tissues were sequenced at three developmental stages during flower opening. Genes that were differentially expressed between the two tissues were filtered for those known to be involved in petal expansion and phytohormone biosynthesis, transport, and signaling, revealing potential roles in petal expansion, especially auxin pathway genes. Using a weighted gene coexpression network analysis (WGCNA), we identified two gene modules that may involve in adaxial–abaxial regulation, 21 and five hub genes have been found respectively. The qRT-PCR validation results were consistent with the RNA-seq data. Based on these findings, we propose a simple network of adaxial–abaxial related genes that regulates petal expansion in R. chinensis ‘Old Blush’. For the first time, we report the adaxial–abaxial transcriptional changes that occur during petal expansion, providing a reference for the study of the regulation of polarity in plant development.

Keywords: Rose petal expansion, auxin, transcription level, RcREV, adaxial–abaxial

Received: 07 Feb 2019; Accepted: 09 Aug 2019.

Copyright: © 2019 Han, Yong, Yu, Cheng, Wang, Yang, Pan and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Qixiang Zhang, Beijing Forestry University, Beijing, China, zqxbjfu@126.com