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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01102

Factors affecting targeted sequencing of 353 nuclear genes from herbarium specimens spanning the diversity of angiosperms

 Grace E. Brewer1*,  James J. Clarkson1*,  Olivier Maurin1*,  Alexandre R. Zuntini1*, Vanessa Barber1,  Sidonie Bellot1, Nicola Biggs1,  Robyn S. Cowan1, Nina M. Davies1,  Steven Dodsworth2, Sara L. Edwards1,  Wolf L. Eiserhardt1, 3, Niroshini Epitawalage1, Sue Frisby1, Aurelie Grall1, Paul J. Kersey1,  Lisa Pokorny1, 4,  Ilia J. Leitch1, Felix Forest1 and  William J. Baker1
  • 1Royal Botanic Gardens, Kew, United Kingdom
  • 2University of Bedfordshire, United Kingdom
  • 3Department of Bioscience, Faculty of Science and Technology, Aarhus University, Denmark
  • 4Center for Plant Biotechnology and Genomics, National Institute of Agricultural and Food Research and Technology, Spain

The world’s herbaria collectively house millions of diverse plant specimens, including endangered or extinct species and type specimens. Unlocking genetic data from the typically highly degraded DNA obtained from herbarium specimens was difficult until the arrival of high-throughput sequencing approaches, which can be applied to low quantities of severely fragmented DNA. Target enrichment involves using short molecular probes that hybridise and capture genomic regions of interest for high-throughput sequencing. In this study on herbariomics, we used this targeted sequencing approach and the Angiosperms353 universal probe set to recover up to 351 nuclear genes from 435 herbarium specimens that are up to 204 years old and span the breadth of angiosperm diversity. We show that on average 207 genes were successfully retrieved from herbarium specimens, although the mean number of genes retrieved and target enrichment efficiency is significantly higher for silica gel-dried specimens. Forty-seven target nuclear genes were recovered from a herbarium specimen of the critically endangered St Helena boxwood, Mellissia begoniifolia, collected in 1815. Herbarium specimens yield significantly less high molecular weight DNA than silica gel-dried specimens, and genomic DNA quality declines with sample age which is negatively correlated with target enrichment efficiency. Climate, taxon-specific traits, and collection strategies additionally impact target sequence recovery. We also detected taxonomic bias in targeted sequencing outcomes for the 10 most numerous angiosperm families that were investigated in depth. We recommend that 1) for species distributed in wet tropical climates, silica gel-dried specimens should be used preferentially, 2) for species distributed in seasonally dry tropical climates, herbarium and silica gel-dried specimens yield similar results, and either collection can be used, 3) taxon specific traits should be explored and established for effective optimisation of taxon-specific studies using herbarium specimens, 4) all herbarium sheets should, in future, be annotated with details of the preservation method used, 5) long-term storage of herbarium specimens should be in stable low humidity and low temperature environments, and 6) targeted sequencing with universal probes, such as Angiosperms353 should be investigated closely as a new approach for DNA barcoding that will ensure better exploitation of herbarium specimens than traditional Sanger sequencing approaches.

Keywords: Herbarium specimens, Degraded DNA, Genomics, High-throughput sequencing (HTS), Target enrichment, Angiosperms, Sample age, herbariomics, DNA bar coding

Received: 07 Jun 2019; Accepted: 12 Aug 2019.

Copyright: © 2019 Brewer, Clarkson, Maurin, Zuntini, Barber, Bellot, Biggs, Cowan, Davies, Dodsworth, Edwards, Eiserhardt, Epitawalage, Frisby, Grall, Kersey, Pokorny, Leitch, Forest and Baker. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence:
Ms. Grace E. Brewer, Royal Botanic Gardens, Kew, Richmond, United Kingdom, g.brewer@kew.org
Dr. James J. Clarkson, Royal Botanic Gardens, Kew, Richmond, United Kingdom, j.clarkson@kew.org
Dr. Olivier Maurin, Royal Botanic Gardens, Kew, Richmond, United Kingdom, o.maurin@kew.org
Dr. Alexandre R. Zuntini, Royal Botanic Gardens, Kew, Richmond, United Kingdom, a.zuntini@kew.org