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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01124

The intracellular transporter AtNRAMP6 is involved in Fe homeostasis in Arabidopsis

 Jiyu Li1, 2, Yuerong Wang1,  Lu Zheng1, Yun Li1, Xueli Zhou1, Jingjun Li1, Dongfang Gu1, Ending Xu1,  Yaping Lu1,  Xi Chen1* and Wei Zhang1
  • 1College of Life Sciences, Nanjing Agricultural University, China
  • 2Anhui Academy of Agricultural Sciences (CAAS), China

Natural resistance-associated macrophage proteins (NRAMPs) have been shown to transport a wide range of divalent metal ions, such as Manganese (Mn), Cadmium (Cd), and Iron (Fe). Iron is an essential micronutrient for plants and Fe deficiency can lead to chlorosis or decreased biomass. AtNRAMP6 has demonstrated the capability to transport Cd, but its physiological function is currently unclear. This study demonstrates that AtNRAMP6 is localized to the Golgi/trans-Golgi network and plays an important role in intracellular Fe homeostasis in the flowering plant genus Arabidopsis. GUS tissue-specific expression revealed that AtNRAMP6 is highly expressed in the lateral roots and young leaves (three to four top leaves) of Arabidopsis. Moreover, knocking out AtNRAMP6 was shown to impair lateral root growth without having a differential effect on the main root under Fe-deficient conditions. Lastly, the expression of AtNRAMP6 was found to exacerbate the sensitivity of the yeast mutant Δccc1 to an excessive amount of Fe. These findings indicate that AtNRAMP6 plays an important role in the growth of Arabidopsis in Fe-deficient conditions.

Keywords: Arabidopsis thaliana, AtNRAMP6, Golgi, trans-Golgi Network, Lateral root growth, Fe homeostasis

Received: 24 May 2019; Accepted: 15 Aug 2019.

Copyright: © 2019 Li, Wang, Zheng, Li, Zhou, Li, Gu, Xu, Lu, Chen and Zhang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Xi Chen, College of Life Sciences, Nanjing Agricultural University, Nanjing, China, xi.chen@njau.edu.cn