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Front. Plant Sci. | doi: 10.3389/fpls.2019.01141

Monitoring of rice transcriptional responses to contrasted colonizing patterns of phytobeneficial Burkholderia s.l. reveals a temporal shift in JA systemic response

 Eoghan King1,  Adrian Wallner1, Isabelle Rimbault1, Celia Barrachina2,  Agnieszka Klonowska1,  Lionel Moulin1 and  Pierre Czernic1*
  • 1IRD UMR186 Interactions Plantes-Microorganismes-Environnement (IPME), France
  • 2Montpellier GenomiX, France

In the context of plant-pathogen and plant-mutualist interactions, the underlying molecular bases associated with host colonization have been extensively studied. However, it is not the case for non-mutualistic beneficial interactions or associative symbiosis with plants. Particularly, little is known about the transcriptional regulations associated with the immune tolerance of plants towards beneficial microbes. In this context, the study of the Burkholderia-rice model is very promising to describe the molecular mechanisms involved in associative symbiosis. Indeed, several species of the Burkholderia sensu lato (s.l.) genus can colonize rice tissues and have beneficial effects; particularly two species have been thoroughly studied: Burkholderia vietnamiensis and Paraburkholderia kururiensis. This study aims at comparing the interaction of these species with rice and especially to identify common or specific plant responses. Therefore, we analyzed root colonization of the rice cultivar Nipponbare using DsRed tagged bacterial strains and produced the transcriptomes of both roots and leaves seven days after root inoculation. This led us to the identification of a co-expression jasmonic acid (JA) related network exhibiting opposite regulation in response to the two strains in the leaves of inoculated plants. We then monitored by qPCR the expression of JA-related genes during time course colonization by each strain. Our results reveal a temporal shift in this JA systemic response, which can be related to different colonization strategies of both strains.

Keywords: RNAseq, endophyte, Symbiosis, Burkholderia, rice, Jasmonic acid

Received: 21 Jun 2019; Accepted: 21 Aug 2019.

Copyright: © 2019 King, Wallner, Rimbault, Barrachina, Klonowska, Moulin and Czernic. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Pierre Czernic, IRD UMR186 Interactions Plantes-Microorganismes-Environnement (IPME), Montpellier, 34394, Languedoc-Roussillon, France,