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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01205

Evolution of the cell wall gene families of grasses

Bryan W. Penning1,  Maureen C. McCann2 and  Nicholas C. Carpita3*
  • 1Corn, Soybean and Wheat Quality Research (USDA-ARS), United States
  • 2Department of Biological Sciences, College of Science, Purdue University, United States
  • 3Botany & Plant Pathology, Purdue University, United States

Grasses and related commelinid monocot species synthesize cell walls distinct in composition from other angiosperm species. With few exceptions, the genomes of all angiosperms contain the genes that encode the enzymes of substrate generation for their polysaccharide, phenylpropanoid and protein constituents. RNAseq analysis of transcripts expressed during development of the upper and lower internodes of maize (Zea mays) stem captured the expression of cell wall-related genes associated with primary or secondary wall formation. High levels of transcript abundances were not confined to genes associated with the distinct walls of grasses, but also of those associated with xyloglucan and pectin synthesis. Combined with proteomics data to confirm that expressed genes are translated, we propose that the distinctive cell wall composition of grasses results from sorting downstream from their sites of synthesis in the Golgi apparatus and hydrolysis of the uncharacteristic polysaccharides, and not from differential expression of synthases of grass-specific polysaccharides.

Keywords: Maize (Zea mays), Cell-wall structure, cell-wall synthesis, type II cell walls, gene annotation, Gene Expression

Received: 06 May 2019; Accepted: 02 Sep 2019.

Copyright: © 2019 Penning, McCann and Carpita. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Nicholas C. Carpita, Purdue University, Botany & Plant Pathology, West Lafayette, 47907, Indiana, United States, carpita@purdue.edu