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Front. Plant Sci. | doi: 10.3389/fpls.2019.01214

A method for evaluating three-dimensional morphological features: a case study using Marchantia polymorpha

 Hirokazu Tsukaya1*,  Tomoyuki Furuya2 and Yoshitaka Kimori3
  • 1Graduate School of Science, The University of Tokyo, Japan
  • 2Graduate School of Science, University of Tokyo, Japan
  • 3National Institute for Basic Biology, Japan

The description and evaluation of morphological features are essential to many biological studies. Bioimaging and quantification methods have been developed to analyze the morphological features of plants. However, efficient three-dimensional (3D) imaging and its quantification are still under development, particularly for studies of plant morphology, due to complex organ structure with great flexibility among individuals with the same genotype. In this study, we propose a new approach that combines a 3D imaging technique using micro-computed tomography and a mathematical image-processing method to describe 3D morphological features. As an example, we applied this method to Marchantia polymorpha, a new model plant used for the evolutional study of land plants, and evaluated a mutant individual with an abnormal 3D shape. Using this new method, we quantitatively described the thallus morphology of M. polymorpha and distinguished the wild type from a mutant with different morphological features. Our newly established method can be applied to various tissues or bodies with irregular 3D morphology.
or bodies with irregular 3D morphology.

Keywords: Liverworts, Marchantia polymorpha, three-dimensional (3D) imaging, Micro-computed tomography (3D-μCT), Mathematical image processing

Received: 12 May 2019; Accepted: 03 Sep 2019.

Copyright: © 2019 Tsukaya, Furuya and Kimori. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Prof. Hirokazu Tsukaya, The University of Tokyo, Graduate School of Science, Bunkyō, 113-0033, Tokyo, Japan, tsukaya@bs.s.u-tokyo.ac.jp