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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01263

Identification and validation of candidate genes involved in fatty acid content in oil palm by genome-wide association analysis

Wei Xia1, Tingting Luo2, Wei Zhang2,  Annaliese Mason3,  Dongyi Huang1,  Xiaolong Huang1, Wengqi Tang1,  Yajing Dou1,  Chunyu Zhang2 and  Yong Xiao4*
  • 1Institute of Tropical Agriculture and Forestry, Hainan University, China
  • 2College of Plant Science and Technology, Huazhong Agricultural University, China
  • 3IFZ Research Centre for Biosystems, Land Use and Nutrition, Justus Liebig University, Germany
  • 4Chinese Academy of Tropical Agricultural Sciences, China

Oil palm (Elaeis guineensis) is the highest yielding oil crop per unit area worldwide, but its oil is considered unhealthy for human consumption due to its high palmitic acid content (C16:0). In order to facilitate breeding for fatty acid content in oil palm, genome-wide association analysis was used to identify and validate SNP markers and underlying candidate genes associated with fatty acid content in a diversity panel of 200 oil palm individuals. A total of 1 261 501 SNP markers had been previously developed according to SLAF-seq (specific locus amplified fragment sequencing). Based on GWAS analysis, 62 SNP markers were significantly associated with fatty acid composition, and in the flanking regions of these SNPs, A total of 223 candidate genes were identified. we found one gene (acyl-ACP thioesterase B genes) that was involved in fatty acid biosynthesis. The gene was associated with high palmic acid contents in the mesocarp. Overexpression the genes caused a significant increase in palmitic acid content.

Keywords: Elaeis guineensis, FATB, fatty acid, genome-wide association analysis, Palmitic Acid

Received: 14 Feb 2019; Accepted: 11 Sep 2019.

Copyright: © 2019 Xia, Luo, Zhang, Mason, Huang, Huang, Tang, Dou, Zhang and Xiao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Yong Xiao, Chinese Academy of Tropical Agricultural Sciences, Haikou, China, xiaoyong1980@catas.cn