%A Ueda,Minoru %A Matsui,Akihiro %A Watanabe,Shunsuke %A Kobayashi,Makoto %A Saito,Kazuki %A Tanaka,Maho %A Ishida,Junko %A Kusano,Miyako %A Seo,Mitsunori %A Seki,Motoaki %D 2019 %J Frontiers in Plant Science %C %F %G English %K epigenetics,histone acetylation,Histone deacetylases (HDAC),stress response,high salinity stress %Q %R 10.3389/fpls.2019.01323 %W %L %M %P %7 %8 2019-October-18 %9 Original Research %+ Prof Motoaki Seki,Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science,Japan,motoaki.seki@riken.jp %+ Prof Motoaki Seki,Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST),Japan,motoaki.seki@riken.jp %+ Prof Motoaki Seki,Plant Epigenome Regulation Laboratory, RIKEN Cluster for Pioneering Research,Japan,motoaki.seki@riken.jp %+ Prof Motoaki Seki,Kihara Institute for Biological Research, Yokohama City University,Japan,motoaki.seki@riken.jp %# %! HDACs antagonism in salt response %* %< %T Transcriptome Analysis of the Hierarchical Response of Histone Deacetylase Proteins That Respond in an Antagonistic Manner to Salinity Stress %U https://www.frontiersin.org/articles/10.3389/fpls.2019.01323 %V 10 %0 JOURNAL ARTICLE %@ 1664-462X %X Acetylation in histone and non-histone proteins is balanced by histone acetyltransferase and histone deacetylase (HDAC) enzymatic activity, an essential aspect of fine-tuning plant response to environmental stresses. HDACs in Arabidopsis are composed of three families (RPD3-like, SIRT, and HD-tuins). A previous study indicated that class I (HDA19) and class II (HDA5/14/15/18) RPD3-like family HDACs control positive and negative responses to salinity stress, respectively. Furthermore, quintuple hda5/14/15/18/19 mutants (quint) exhibit salinity stress tolerance, suggesting that hda19 suppresses the sensitivity to salinity stress present in quadruple hda5/14/15/18 mutants (quad). In the present study, transcriptome analysis of the quint mutant was conducted to elucidate the hierarchical control of salinity stress response operated by RPD3-like family HDACs (HDA5/14/15/18/19). The analysis identified 4,832 salt-responsive genes in wild-type (Col-0), hda19-3, quad, and quint plants and revealed that 56.7% of the salt-responsive genes exhibited a similar expression pattern in both the hda19-3 and quint plants. These results indicate that deficiency in HDA19 has a bigger impact on salinity stress response than in class II HDACs. Furthermore, the expression pattern of genes encoding enzymes that metabolize phytohormones raises the possibility that a drastic change in the homeostasis of phytohormones, such as abscisic acid, brassinosteroid, and gibberellin, may contribute to increasing stress tolerance in hda19-3 and quint plants. Among these phytohormones, abscisic acid accumulation actually increased in hda19-3 and quint plants, and decreased in quad, compared with wild-type plants. Importantly, 7.8% of the salt-responsive genes in quint plants exhibited a similar expression pattern in quad plants, suggesting that some gene sets are regulated in an HDA5/14/15/18-dependent manner. The transcriptome analysis conducted in the present study revealed the hierarchical and independent regulation of salt stress response that is mediated through HDA19 and class II HDACs.