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Original Research ARTICLE Provisionally accepted The full-text will be published soon. Notify me

Front. Plant Sci. | doi: 10.3389/fpls.2019.01341

Two loci, RiAF3 and RiAF4, contribute to the Annual-Fruiting trait in Rubus

  • 1New Cultivar Innovation, The New Zealand Institute for Plant & Food Research Ltd, New Zealand
  • 2Blue berry genetics and genomics, North Carolina State University, United States
  • 3Department of Horticultural Science, North Carolina State University, United States
  • 4Institute of Agrifood Research and Technology (IRTA), Spain
  • 5Centre for Research in Agricultural Genomics (CRAG), Spain
  • 6New Cultivar Innovation, Brockmann Consult (Germany), Germany
  • 7New Cultivar Innovation, The New Zealand Institute for Plant & Food Research Ltd, New Zealand
  • 8Plant and Food, The New Zealand Institute for Plant & Food Research Ltd, New Zealand

Most Rubus species have a biennial cycle of flowering and fruiting with an intervening period of winter dormancy, in common with many perennial fruit crops. Annual-Fruiting varieties of raspberry (R. idaeus and R. occidentalis L.) and blackberry (R. subgenus Rubus) are able to flower and fruit in one growing season, without the intervening dormant period normally required in Biennial-Fruiting (BF) varieties. We used a red raspberry (R. idaeus) population segregating for AF obtained from a cross between NC493 and ‘Chilliwack’ to identify genetic factors controlling AF. Genotyping by sequencing was used to generate saturated linkage maps in both parents. Trait mapping in this population indicated that AF is controlled by two newly identified loci (RiAF3 and RiAF4) located on Rubus linkage groups (LGs) 3 and 4. The location of these loci was analyzed using single nucleotide polymorphism (SNPs) markers on independent red raspberry and blackberry populations segregating for the AF trait. This confirmed that AF in Rubus is regulated by loci on LG 3 and 4, in addition to a previously reported locus on LG 7. Comparative RNAseq analysis at the time of floral bud differentiation in an AF and a BF variety revealed candidate genes potentially regulating the trait.

Keywords: marker assisted selection (MAS), Primocane fruiting, Floricane-fruiting, comparative mapping, rna sequence, annual-fruiting, Biennial-fruiting

Received: 11 Jun 2019; Accepted: 26 Sep 2019.

Copyright: © 2019 Jibran, Spencer, Fernandez, Monfort, Dzierzon, Tahir, Davies, Chagné and Foster. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

* Correspondence: Dr. Toshi M. Foster, The New Zealand Institute for Plant & Food Research Ltd, Plant and Food, Auckland, 4474, New Zealand,