Original Research ARTICLE
High-density Genetic Map Construction and QTL Maping of Phenotype Related Traits in Ziziphus jujuba Mill
- 1Northwest A&F University, China
- 2Shandong Institute of Pomology, China
The Chinese jujube (Ziziphus jujuba Mill., 2n = 2 x = 24), one of the most popular fruit trees in Asia, is widely cultivated and utilized in China, where it is traditionally consumed as both fresh and dried food sources. A high-density genetic map can provide the necessary framework for quantitative trait loci (QTL) analyses, map-based gene cloning, and molecular breeding. In this study, we constructed a new high-density genetic linkage map via a genotyping-by-sequencing (GBS) approach. For the consensus linkage map, a total of 3792 markers spanning 2167.5 cM were mapped onto 12 linkage groups, with an average marker distance of 0.358cM. The genetic map anchored 301 Mb (85.7%) of scaffolds from the sequenced Z. jujuba ‘Junzao’ genome. Based on this genetic map, 30 potential QTLs were detected, including 27 for leaf traits and 3 for needling length. This high-density genetic map and the identified QTLs for relevant agronomic traits lay the groundwork for functional genetic mapping, map-based cloning and marker-assisted selection (MAS) in the jujube.
Keywords: Jujube (Ziziphus jujuba Mill),, GBS (genotyping-by-sequencing), Geneticmap, phenotype traits, QTL
Received: 09 Nov 2018;
Accepted: 14 Oct 2019.
Copyright: © 2019 Wang, Zhang, Tang, Zhang, Zhou and Li. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Mr. Guangfang Zhou, Shandong Institute of Pomology, Taian, China, firstname.lastname@example.org
Prof. Xingang Li, Northwest A&F University, Yangling, 712100, Shaanxi Province, China, email@example.com